WB (Western Blot)
(Anti-TRIF antibody, AAA45389, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Mouse TICAM1,37.5KD(162aa tag+ L551-E732)Lane 1: Recombinant Mouse TICAM1 Protein 10ngLane 2: Recombinant Mouse TICAM1 Protein 5ngLane 3: Recombinant Mouse TICAM1 Protein 2.5ngLane 4: Recombinant Mouse TICAM1 Protein 1.25ng)
Rabbit anti-Mouse TRIF Polyclonal Antibody | anti-TICAM1 antibody
Anti-TRIF antibody
Gene Names
Ticam1; TRIF; TICAM-1; AW046014; AW547018
Reactivity
Mouse
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
TRIF, Antibody; Anti-TRIF antibody; TIR domain-containing adapter molecule 1; toll-like receptor adaptor molecule 1; MGC35334 antibody; Proline rich vinculin and TIR domain containing protein B antibody; Proline-rich antibody; PRVTIRB antibody; Putative NF kappa B activating protein 502H antibody; Putative NF-kappa-B-activating protein 502H antibody; Putative NFkB activating protein antibody; TCAM1_HUMAN antibody; TICAM 1 antibody; TICAM-1 antibody; TICAM1 antibody; TIR domain containing adapter molecule 1 antibody; TIR domain containing adapter protein inducing IFN beta antibody; TIR domain containing adaptor inducing interferon beta antibody; TIR domain-containing adapter molecule 1 antibody; TIR domain-containing adapter protein inducing IFN-beta antibody; Toll interleukin 1 receptor domain containing adapter protein inducing interferon beta antibody; Toll like receptor adaptor molecule 1 antibody; Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta antibody; TRIF antibody; TRIF protein antibody; vinculin and TIR domain-containing protein B antibody; anti-TICAM1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
732
Applicable Applications for anti-TICAM1 antibody
WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse TICAM1 (693-708aa NNHMWGHTGAQSSDDK).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-TRIF antibody, AAA45389, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Mouse TICAM1,37.5KD(162aa tag+ L551-E732)Lane 1: Recombinant Mouse TICAM1 Protein 10ngLane 2: Recombinant Mouse TICAM1 Protein 5ngLane 3: Recombinant Mouse TICAM1 Protein 2.5ngLane 4: Recombinant Mouse TICAM1 Protein 1.25ng)
WB (Western Blot)
(Anti-TRIF antibody, AAA45389, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Mouse TICAM1,37.5KD(162aa tag+ L551-E732)Lane 1: Recombinant Mouse TICAM1 Protein 10ngLane 2: Recombinant Mouse TICAM1 Protein 5ngLane 3: Recombinant Mouse TICAM1 Protein 2.5ngLane 4: Recombinant Mouse TICAM1 Protein 1.25ng)
Related Product Information for anti-TICAM1 antibody
Description: Rabbit IgG polyclonal antibody for TIR domain-containing adapter molecule 1(TICAM1) detection. Tested with WB in Mouse.
Background: TICAM1(TIR DOMAIN-CONTAINING ADAPTOR MOLECULE 1), also known as TRIF, is an adapter in responding to activation of toll-like receptors(TLRs). It mediates the rather delayed cascade of two TLR-associated signaling cascades, where the other one is dependent upon a MyD88 adapter. By genomic sequence analysis, Oshiumi et al.(2003) mapped the TICAM1 gene to chromosome 19p13.3. By coimmunoprecipitation analysis, Oshiumi et al.(2003) showed that TICAM1 interacts specifically with TLR3, but not with other TLRs. Functional analysis showed that the association of TLR3 and TICAM1 mediates dsRNA activation of IFNB, through either NFKB, AP1, or IRF3. TICAM1 activation of NFKB was found to occur predominantly through IRAK1 rather than IRAK2. Small interfering(si)RNA blockage of TICAM1, just upstream of the TIR domain, reduced IFNB production in response to dsRNA.
Background: TICAM1(TIR DOMAIN-CONTAINING ADAPTOR MOLECULE 1), also known as TRIF, is an adapter in responding to activation of toll-like receptors(TLRs). It mediates the rather delayed cascade of two TLR-associated signaling cascades, where the other one is dependent upon a MyD88 adapter. By genomic sequence analysis, Oshiumi et al.(2003) mapped the TICAM1 gene to chromosome 19p13.3. By coimmunoprecipitation analysis, Oshiumi et al.(2003) showed that TICAM1 interacts specifically with TLR3, but not with other TLRs. Functional analysis showed that the association of TLR3 and TICAM1 mediates dsRNA activation of IFNB, through either NFKB, AP1, or IRF3. TICAM1 activation of NFKB was found to occur predominantly through IRAK1 rather than IRAK2. Small interfering(si)RNA blockage of TICAM1, just upstream of the TIR domain, reduced IFNB production in response to dsRNA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
TIR domain-containing adapter molecule 1
NCBI Official Synonym Full Names
toll-like receptor adaptor molecule 1
NCBI Official Symbol
Ticam1
NCBI Official Synonym Symbols
TRIF; TICAM-1; AW046014; AW547018
NCBI Protein Information
TIR domain-containing adapter molecule 1; TIR domain-containing adaptor inducing interferon-beta; TIR domain-containing adapter protein inducing IFN-beta; toll-interleukin 1 receptor domain-containing adapter protein inducing interferon-beta; toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta
UniProt Protein Name
TIR domain-containing adapter molecule 1
UniProt Gene Name
Ticam1
UniProt Synonym Gene Names
Trif; TICAM-1; TIR domain-containing adapter protein inducing IFN-beta
UniProt Entry Name
TCAM1_MOUSE
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Product Notes
The TICAM1 ticam1 (Catalog #AAA45389) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TRIF antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TRIF can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the TICAM1 ticam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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