IHC (Immunohistochemisry)
(Immunofluorescent analysis of Hela cells using AAA232855 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Rabbit anti-Human TRIM25 Polyclonal Antibody | anti-TRIM25 antibody
TRIM25 Antibody
Gene Names
TRIM25; EFP; Z147; RNF147; ZNF147
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
TRIM25, Antibody; TRIM25 Antibody; E3 ubiquitin/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; RING-type E3 ubiquitin transferase (EC: 2.3.2.271; TRIM25; EFP; RNF147; ZNF147; anti-TRIM25 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
630
Applicable Applications for anti-TRIM25 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant Human E3 ubiquitin/ISG15 ligase TRIM25 protein (81-272AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-22832 / sc-30890
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IHC (Immunohistochemisry)
(Immunofluorescent analysis of Hela cells using AAA232855 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemisry)
(Immunofluorescent analysis of Hela cells using AAA232855 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human colon cancer using AAA232855 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human colon cancer using AAA232855 at dilution of 1:100)
WB (Western Blot)
(Western BlotPositive WB detected in:MCF-7 whole cell lysate,Jurkat whole cell lysate,Hela whole cell lysate,K562 whole cell lysateAll lanes: TRIM25 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 71 kDaObserved band size: 71,80 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in:MCF-7 whole cell lysate,Jurkat whole cell lysate,Hela whole cell lysate,K562 whole cell lysateAll lanes: TRIM25 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 71 kDaObserved band size: 71,80 kDa)
Related Product Information for anti-TRIM25 antibody
Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed: 22452784).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
70,973 Da
NCBI Official Full Name
E3 ubiquitin/ISG15 ligase TRIM25
NCBI Official Synonym Full Names
tripartite motif containing 25
NCBI Official Symbol
TRIM25
NCBI Official Synonym Symbols
EFP; Z147; RNF147; ZNF147
NCBI Protein Information
E3 ubiquitin/ISG15 ligase TRIM25
UniProt Protein Name
E3 ubiquitin/ISG15 ligase TRIM25
UniProt Gene Name
TRIM25
UniProt Synonym Gene Names
EFP; RNF147; ZNF147
UniProt Entry Name
TRI25_HUMAN
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Product Notes
The TRIM25 trim25 (Catalog #AAA232855) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIM25 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM25 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the TRIM25 trim25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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