WB (Western Blot)
(Detection of human TRIM25 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, MCF-7, LNCaP, A-549, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIM25 antibody AAA211657 (lot AAA211657-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human TRIM25 Polyclonal Antibody | anti-TRIM25 antibody
Rabbit anti-TRIM25 Antibody, Affinity Purified
Gene Names
TRIM25; EFP; Z147; RNF147; ZNF147
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
TRIM25, Antibody; Rabbit anti-TRIM25 Antibody, Affinity Purified; ubiquitin/ISG15-conjugating enzyme TRIM25; RNF147; ZNF147; zinc finger protein-147; zinc finger protein 147 (estrogen-responsive finger protein); Zinc finger protein 147; Z147; E3 ubiquitin/ISG15 ligase TRIM25; tripartite motif-containing protein 25; RING-type E3 ubiquitin transferase TRIM25; RING-type E3 ubiquitin transferase; RING finger protein 147; estrogen-responsive finger protein; EFP; tripartite motif protein TRIM25; tripartite motif containing 25; anti-TRIM25 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-TRIM25 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 350 and 400
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human TRIM25 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, MCF-7, LNCaP, A-549, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIM25 antibody AAA211657 (lot AAA211657-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human TRIM25 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, MCF-7, LNCaP, A-549, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIM25 antibody AAA211657 (lot AAA211657-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human TRIM25 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIM25 antibody AAA211657 (lot AAA211657-3) used for IP at 6 ug per reaction. TRIM25 was also immunoprecipitated by rabbit anti-TRIM25 antibody For blotting immunoprecipitated TRIM25, AAA211657 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human TRIM25 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIM25 antibody AAA211657 (lot AAA211657-3) used for IP at 6 ug per reaction. TRIM25 was also immunoprecipitated by rabbit anti-TRIM25 antibody For blotting immunoprecipitated TRIM25, AAA211657 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-TRIM25 antibody
TRIM (tripartite motif) proteins, also known as RBCC (RING, B-box, coiled-coil) proteins, are defined by the presence of a tripartite structural motif composed of a RING domain, one or two B-box motifs, and a coiled-coil region. TRIM proteins have been found to be involved in a variety of cellular processes such as apoptosis, proliferation, differentiation, and innate immunity. Of these processes, the most intriguing is their involvement in the innate immune response. TRIM25 has been demonstrated to mediate ubiquitination of the cytosolic receptor retinoic acid-inducible gene-I (RIG-I) in response to viral infection.
Product Categories/Family for anti-TRIM25 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
70,973 Da
NCBI Official Full Name
E3 ubiquitin/ISG15 ligase TRIM25
NCBI Official Synonym Full Names
tripartite motif containing 25
NCBI Official Symbol
TRIM25
NCBI Official Synonym Symbols
EFP; Z147; RNF147; ZNF147
NCBI Protein Information
E3 ubiquitin/ISG15 ligase TRIM25; RING finger protein 147; zinc finger protein-147; tripartite motif-containing 25; tripartite motif protein TRIM25; estrogen-responsive finger protein; tripartite motif-containing protein 25; ubiquitin/ISG15-conjugating en
UniProt Protein Name
E3 ubiquitin/ISG15 ligase TRIM25
UniProt Gene Name
TRIM25
UniProt Synonym Gene Names
EFP; RNF147; ZNF147
UniProt Entry Name
TRI25_HUMAN
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Product Notes
The TRIM25 trim25 (Catalog #AAA211657) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-TRIM25 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM25 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the TRIM25 trim25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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