WB (Western Blot)
(TRIM33 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the TRIM33 antibody detected the TRIM33 protein (arrow).)
Rabbit anti-Human TRIM33 Polyclonal Antibody | anti-TRIM33 antibody
TRIM33 Antibody (C-term)
Gene Names
TRIM33; ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TRIM33, Antibody; TRIM33 Antibody (C-term); E3 ubiquitin-protein ligase TRIM33; 632-; Ectodermin homolog; RET-fused gene 7 protein; Protein Rfg7; Transcription intermediary factor 1-gamma; TIF1-gamma; Tripartite motif-containing protein 33; TRIM33; KIAA1113; RFG7; TIF1G; anti-TRIM33 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TRIM33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 820-849 amino acids from the C-terminal region of human TRIM33.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
820-849
Sequence Length
1,110
Applicable Applications for anti-TRIM33 antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Cellular Location
Nucleus. Note=In discrete nuclear dots resembling nuclear bodies.
Tissue Location
Expressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(TRIM33 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the TRIM33 antibody detected the TRIM33 protein (arrow).)
WB (Western Blot)
(TRIM33 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the TRIM33 antibody detected the TRIM33 protein (arrow).)
Related Product Information for anti-TRIM33 antibody
The protein encoded by this gene is thought to be a
transcriptional corepressor. However, molecules that interact with
this protein have not yet been identified. The protein is a member
of the tripartite motif family. This motif includes three
zinc-binding domains, a RING, a B-box type 1 and a B-box type 2,
and a coiled-coil region. Three alternatively spliced transcript
variants for this gene have been described, however, the
full-length nature of one variant has not been determined.
Function: Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF- beta/BMP signaling cascade).
transcriptional corepressor. However, molecules that interact with
this protein have not yet been identified. The protein is a member
of the tripartite motif family. This motif includes three
zinc-binding domains, a RING, a B-box type 1 and a B-box type 2,
and a coiled-coil region. Three alternatively spliced transcript
variants for this gene have been described, however, the
full-length nature of one variant has not been determined.
Function: Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF- beta/BMP signaling cascade).
Product Categories/Family for anti-TRIM33 antibody
References
Bai, X., et al. Cell 142(1):133-143(2010)
Howard, P.W., et al. Biochem. Biophys. Res. Commun. 396(3):674-678(2010)
Vincent, D.F., et al. PLoS Genet. 5 (7), E1000575 (2009) :
Dupont, S., et al. Cell 136(1):123-135(2009)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Howard, P.W., et al. Biochem. Biophys. Res. Commun. 396(3):674-678(2010)
Vincent, D.F., et al. PLoS Genet. 5 (7), E1000575 (2009) :
Dupont, S., et al. Cell 136(1):123-135(2009)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
122533
NCBI Official Full Name
E3 ubiquitin-protein ligase TRIM33 isoform alpha
NCBI Official Synonym Full Names
tripartite motif containing 33
NCBI Official Symbol
TRIM33
NCBI Official Synonym Symbols
ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA
NCBI Protein Information
E3 ubiquitin-protein ligase TRIM33
UniProt Protein Name
E3 ubiquitin-protein ligase TRIM33
UniProt Gene Name
TRIM33
UniProt Synonym Gene Names
KIAA1113; RFG7; TIF1G; Protein Rfg7; TIF1-gamma
UniProt Entry Name
TRI33_HUMAN
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Product Notes
The TRIM33 trim33 (Catalog #AAA289402) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 820-849. The TRIM33 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM33 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TRIM33 trim33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM33, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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