WB (Western Blot)
(Detection of human TRIP4/ASC-1 by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, Jurkat, A-549, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP4-ASC-1 antibody (AAA211175 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human, Mouse TRIP4/ASC-1 Polyclonal Antibody | anti-TRIP4/ASC-1 antibody
Rabbit anti-TRIP4/ASC-1 Antibody, Affinity Purified
Gene Names
TRIP4; ASC1; ASC-1; HsT17391
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
TRIP4/ASC-1, Antibody; Rabbit anti-TRIP4/ASC-1 Antibody, Affinity Purified; SMABF1; zinc finger, C2HC5-type; ZC2HC5; TRIP-4; thyroid receptor-interacting protein 4; MDCDC; HsT17391; ASC-1; ASC1; activating signal cointegrator 1; TR-interacting protein 4; Activating signal cointegrator 1; thyroid hormone receptor interactor 4; anti-TRIP4/ASC-1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-TRIP4/ASC-1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 225 and 275
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human TRIP4/ASC-1 by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, Jurkat, A-549, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP4-ASC-1 antibody (AAA211175 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human TRIP4/ASC-1 by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, Jurkat, A-549, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP4-ASC-1 antibody (AAA211175 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of mouse TRIP4/ASC-1 by western blot. Samples: Whole cell lysate (25 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP4/ASC-1 antibody (AAA211175 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse TRIP4/ASC-1 by western blot. Samples: Whole cell lysate (25 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP4/ASC-1 antibody (AAA211175 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human TRIP4/ASC-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIP4/ASC-1 antibody (AAA211175 lot 4) used for IP at 6 ug per reaction. TRIP4/ASC-1 was also immunoprecipitated by a previous lot of this antibody (AAA211175 lot 3) and a second antibody against a different epitope of TRIP4/ASC-1 . For blotting immunoprecipitated TRIP4/ASC-1, AAA211175 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human TRIP4/ASC-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIP4/ASC-1 antibody (AAA211175 lot 4) used for IP at 6 ug per reaction. TRIP4/ASC-1 was also immunoprecipitated by a previous lot of this antibody (AAA211175 lot 3) and a second antibody against a different epitope of TRIP4/ASC-1 . For blotting immunoprecipitated TRIP4/ASC-1, AAA211175 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-TRIP4/ASC-1 antibody
Thyroid receptor interacting protein 4 (TRIP4) is also known as activating signal cointegrator 1 (ASC-1). TRIP4/ASC-1 was identified as a protein that interacts with the thyroid receptor. It has been shown to act as a transcriptional co-activator of the thyroid receptor (TR), 9-cis retinoic acid (RXR), androgen receptor (AR) and estrogen.
Product Categories/Family for anti-TRIP4/ASC-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,146 Da
NCBI Official Full Name
activating signal cointegrator 1
NCBI Official Synonym Full Names
thyroid hormone receptor interactor 4
NCBI Official Symbol
TRIP4
NCBI Official Synonym Symbols
ASC1; ASC-1; HsT17391
NCBI Protein Information
activating signal cointegrator 1; TRIP-4; TR-interacting protein 4; thyroid receptor interacting protein 4; thyroid receptor-interacting protein 4
UniProt Protein Name
Activating signal cointegrator 1
UniProt Gene Name
TRIP4
UniProt Synonym Gene Names
ASC-1; TR-interacting protein 4; TRIP-4
UniProt Entry Name
TRIP4_HUMAN
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Product Notes
The TRIP4/ASC-1 trip4 (Catalog #AAA211175) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-TRIP4/ASC-1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TRIP4/ASC-1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the TRIP4/ASC-1 trip4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIP4/ASC-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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