IHC (Immunohiostchemistry)
(TRPM4 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- TRPM4 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
anti-Human, Mouse TRPM4 Polyclonal Antibody | anti-TRPM4 antibody
Anti-TRPM4 Antibody
Gene Names
TRPM4; LTrpC4; PFHB1B; TRPM4B; hTRPM4
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
TRPM4, Antibody; Anti-TRPM4 Antibody; Transient receptor potential cation channel subfamily M member 4; 1110030C19Rik; AW047689; Calcium-activated non-selective cation channel 1; FLJ20041; hTRPM4; Long transient receptor potential channel 4; LTrpC-4; LTrpC4; Melastatin 4; Melastatin like 2 protein; Melastatin-4; Melastatin-like 2; Mls2s; PFHB1B; Transient receptor potential cation channel, subfamily M, member 4; Trpm4; TRPM4_HUMAN; TRPM4B; transient receptor potential cation channel subfamily M member 4; anti-TRPM4 antibody
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
1069
Applicable Applications for anti-TRPM4 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human TRPM4 recombinant protein (Position: F1079-D1214). Human TRPM4 shares 76.8% and 77.8% amino acid (aa) sequence identity with mouse and rat TRPM4, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(TRPM4 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- TRPM4 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohiostchemistry)
(TRPM4 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- TRPM4 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
WB (Western Blot)
(Western blot analysis of TRPM4 expression in mouse spleen extract (lane 1) and SW620 whole cell lysates (lane 2). TRPM4 at 134KD was detected using rabbit anti- TRPM4 Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.)
WB (Western Blot)
(Western blot analysis of TRPM4 expression in mouse spleen extract (lane 1) and SW620 whole cell lysates (lane 2). TRPM4 at 134KD was detected using rabbit anti- TRPM4 Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.)
Related Product Information for anti-TRPM4 antibody
Description: Rabbit IgG polyclonal antibody for Transient receptor potential cation channel subfamily M member 4(TRPM4) detection. Tested with WB, IHC-P in Human;Mouse.
Background: Transient receptor potential cation channel subfamily M member 4 (hTRPM4), also known as melastatin-4, is a protein that in humans is encoded by the TRPM4 gene. It is mapped to 19q13.33. The protein encoded by this gene is a calcium-activated nonselective ion channel that mediates transport of monovalent cations across membranes, thereby depolarizing the membrane. The activity of the encoded protein increases with increasing intracellular calcium concentration, but this channel does not transport calcium. Two transcript variants encoding different isoforms have been found for this gene.
Background: Transient receptor potential cation channel subfamily M member 4 (hTRPM4), also known as melastatin-4, is a protein that in humans is encoded by the TRPM4 gene. It is mapped to 19q13.33. The protein encoded by this gene is a calcium-activated nonselective ion channel that mediates transport of monovalent cations across membranes, thereby depolarizing the membrane. The activity of the encoded protein increases with increasing intracellular calcium concentration, but this channel does not transport calcium. Two transcript variants encoding different isoforms have been found for this gene.
References
1. Clapham DE, Julius D, Montell C, Schultz G (December 2005). "International Union of Pharmacology. XLIX. Nomenclature and structure-function relationships of transient receptor potential channels". Pharmacol. Rev.57 (4): 427-50. 2. Xu XZ, Moebius F, Gill DL, Montell C (September 2001). "Regulation of melastatin, a TRP-related protein, through interaction with a cytoplasmic isoform". Proc. Natl. Acad. Sci. U.S.A. 98 (19): 10692-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
118,630 Da
NCBI Official Full Name
transient receptor potential cation channel subfamily M member 4 isoform 2
NCBI Official Synonym Full Names
transient receptor potential cation channel subfamily M member 4
NCBI Official Symbol
TRPM4
NCBI Official Synonym Symbols
LTrpC4; PFHB1B; TRPM4B; hTRPM4
NCBI Protein Information
transient receptor potential cation channel subfamily M member 4
UniProt Protein Name
Transient receptor potential cation channel subfamily M member 4
UniProt Gene Name
TRPM4
UniProt Synonym Gene Names
LTRPC4; hTRPM4; LTrpC-4; LTrpC4
UniProt Entry Name
TRPM4_HUMAN
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Product Notes
The TRPM4 trpm4 (Catalog #AAA46345) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-TRPM4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TRPM4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TRPM4 trpm4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRPM4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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