WB (Western Blot)
(Western blot analysis of TRPV4 using HeLa whole cell lysates)
Rabbit TRPV4 Polyclonal Antibody | anti-TRPV4 antibody
TRPV4 Antibody
Gene Names
TRPV4; SMAL; VRL2; BCYM3; CMT2C; SPSMA; TRP12; VROAC; HMSN2C; OTRPC4; SSQTL1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TRPV4, Antibody; TRPV4 Antibody; BCYM3; CMT2C; HMSN2C; osm 9 like TRP channel 4; Osm-9-like TRP channel 4; OSM9 like transient receptor potential channel 4; Osmosensitive transient receptor potential channel 4; OTRPC 4; OTRPC4; SMAL; SPSMA; SSQTL1; Transient receptor potential cation channel subfamily V member 4; Transient receptor potential protein 12; TRP 12; TRP12; TRPV 4; TrpV4; TRPV4_HUMAN; Vanilloid receptor like channel 2; Vanilloid receptor like protein 2; Vanilloid receptor related osmotically activated channel; Vanilloid receptor-like channel 2; Vanilloid receptor-like protein 2; Vanilloid receptor-related osmotically-activated channel; VR 4; VR OAC; VR-OAC; VR4; VRL 2; VRL-2; VRL2; VROAC; anti-TRPV4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
TRPV4 antibody detects endogenous levels of total TRPV4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
824
Applicable Applications for anti-TRPV4 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human TRPV4
Subcellular Location
Cell Membrane and Cell Membrane. Cell Junction > Adherens Junction. Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum.
Tissue Specificity
Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Sheep, Rabbit
Similarity
Pig (100%), Zebrafish (82%), Bovine (100%), Sheep (100%), Rabbit (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of TRPV4 using HeLa whole cell lysates)
WB (Western Blot)
(Western blot analysis of TRPV4 using HeLa whole cell lysates)
WB (Western Blot)
(Western blot analysis of extracts from rat brain, using TRPV4 Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from rat brain, using TRPV4 Antibody.)
Related Product Information for anti-TRPV4 antibody
Function: Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification (PubMed:18826956, PubMed:18695040). Also activated by heat, low pH, citrate and phorbol esters (PubMed:16293632, PubMed:18826956, PubMed:18695040, PubMed:25256292, PubMed:20037586, PubMed:21964574). Increase of intracellular Ca2+ potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism (PubMed:12724311, PubMed:18826956). Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (By similarity). Acts as a regulator of intracellular Ca2+ in synoviocytes (PubMed:19759329). Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12, 13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (PubMed:19759329). Together with PKD2, forms mechano-and thermosensitive channels in cilium (PubMed:18695040). Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (By similarity). Regulates expression of chemokines and cytokines related to proinflammatory pathway in adipocytes (By similarity). Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (By similarity). Required for normal development and maintenance of bone and cartilage (PubMed:26249260).
Subunit Structure: Homotetramer (Probable). Self-associates in a isoform-specific manner (PubMed:16293632). Isoform 1 and isoform 5 can oligomerize, but isoform 2, isoform 4 and isoform 6 cannnot oligomerize (PubMed:16293632). Interacts with calmodulin (PubMed:12724311). Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES (By similarity). Interacts with CTNNB1 (By similarity). The TRPV4 and CTNNB1 complex can interact with CDH1 (By similarity). Interacts with PACSIN1, PACSIN2 and PACSIN3 (via SH3 domain) (By similarity). Part of a complex containing MLC1, AQP4, HEPACAM and ATP1B1 (PubMed:22328087). Interacts with ITPR3 (PubMed:18826956). Interacts with AQP5; the interaction is probably indirect and regulates TRPV4 activation by hypotonicity (By similarity). Interacts with ANO1 (By similarity). Interacts (via C-terminus) with PKD2 (via C-terminus) (PubMed:18695040).
Post-translational Modifications: N-glycosylated.
Similarity: The ANK repeat region mediates interaction with Ca2+-calmodulin and ATP binding (By similarity). The ANK repeat region mediates interaction with phosphatidylinositol-4, 5-bisphosphate and related phosphatidylinositides (PubMed:25256292). Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily.
Subunit Structure: Homotetramer (Probable). Self-associates in a isoform-specific manner (PubMed:16293632). Isoform 1 and isoform 5 can oligomerize, but isoform 2, isoform 4 and isoform 6 cannnot oligomerize (PubMed:16293632). Interacts with calmodulin (PubMed:12724311). Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES (By similarity). Interacts with CTNNB1 (By similarity). The TRPV4 and CTNNB1 complex can interact with CDH1 (By similarity). Interacts with PACSIN1, PACSIN2 and PACSIN3 (via SH3 domain) (By similarity). Part of a complex containing MLC1, AQP4, HEPACAM and ATP1B1 (PubMed:22328087). Interacts with ITPR3 (PubMed:18826956). Interacts with AQP5; the interaction is probably indirect and regulates TRPV4 activation by hypotonicity (By similarity). Interacts with ANO1 (By similarity). Interacts (via C-terminus) with PKD2 (via C-terminus) (PubMed:18695040).
Post-translational Modifications: N-glycosylated.
Similarity: The ANK repeat region mediates interaction with Ca2+-calmodulin and ATP binding (By similarity). The ANK repeat region mediates interaction with phosphatidylinositol-4, 5-bisphosphate and related phosphatidylinositides (PubMed:25256292). Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 98 kDa
Predicted: 99 kDa
Predicted: 99 kDa
NCBI Official Full Name
transient receptor potential cation channel subfamily V member 4 isoform e
NCBI Official Synonym Full Names
transient receptor potential cation channel subfamily V member 4
NCBI Official Symbol
TRPV4
NCBI Official Synonym Symbols
SMAL; VRL2; BCYM3; CMT2C; SPSMA; TRP12; VROAC; HMSN2C; OTRPC4; SSQTL1
NCBI Protein Information
transient receptor potential cation channel subfamily V member 4
UniProt Protein Name
Transient receptor potential cation channel subfamily V member 4
UniProt Gene Name
TRPV4
UniProt Synonym Gene Names
VRL2; VROAC; TRP12; VRL-2; VR-OAC1 PublicationManual assertion based on opinion iniRef.1
Similar Products
Product Notes
The TRPV4 trpv4 (Catalog #AAA327554) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRPV4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TRPV4 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the TRPV4 trpv4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRPV4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.