WB (Western Blot)
(WB Suggested Anti-TRPV4 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:12500Positive Control: ACHN cell lysate)
Rabbit TRPV4 Polyclonal Antibody | anti-TRPV4 antibody
TRPV4 antibody - middle region
Gene Names
TRPV4; SMAL; VRL2; BCYM3; CMT2C; SPSMA; TRP12; VROAC; HMSN2C; OTRPC4; SSQTL1
Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Synonyms
TRPV4, Antibody; TRPV4 antibody - middle region; anti-TRPV4 antibody
Host
Rabbit
Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: RVDEVNWSHWNQNLGIINEDPGKNETYQYYGFSHTVGRLRRDRWSSVVPR
Sequence Length
811
Applicable Applications for anti-TRPV4 antibody
WB (Western Blot), IHC (Immunohistochemistry)
Homology
Cow: 93%; Dog: 86%; Guinea Pig: 86%; Human: 100%; Mouse: 86%; Pig: 93%; Rabbit: 100%; Rat: 93%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human TRPV4
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(WB Suggested Anti-TRPV4 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:12500Positive Control: ACHN cell lysate)
WB (Western Blot)
(WB Suggested Anti-TRPV4 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:12500Positive Control: ACHN cell lysate)
IHC (Immunohistochemistry)
(TRPV4 Rat HippocampusWe tested the antibodies in Immunohistochemistry studies. The dilutions for all the above primary antibodies were 1:500 and the same for the secondary antibody, Alexa Fluor 568-conjugated goat anti-rabbit antibodies (Invitrogen).)
IHC (Immunohistochemistry)
(TRPV4 Rat HippocampusWe tested the antibodies in Immunohistochemistry studies. The dilutions for all the above primary antibodies were 1:500 and the same for the secondary antibody, Alexa Fluor 568-conjugated goat anti-rabbit antibodies (Invitrogen).)
Related Product Information for anti-TRPV4 antibody
This is a rabbit polyclonal antibody against TRPV4. It was validated on Western Blot using a cell lysate as a positive control.
Target Description: TRPV4 is a non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation of TRPV4 by exposure to hypotonicity within the physiological range exhibits an outward rectification. TRPV4 also activated by low pH, citrate and phorbol esters and increase of intracellular Ca2+ potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism.Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3) This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Two transcript variants encoding different isoforms have been found for this gene.
Target Description: TRPV4 is a non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation of TRPV4 by exposure to hypotonicity within the physiological range exhibits an outward rectification. TRPV4 also activated by low pH, citrate and phorbol esters and increase of intracellular Ca2+ potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism.Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3) This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Two transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-TRPV4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
91kDa
NCBI Official Full Name
transient receptor potential cation channel subfamily V member 4 isoform b
NCBI Official Synonym Full Names
transient receptor potential cation channel subfamily V member 4
NCBI Official Symbol
TRPV4
NCBI Official Synonym Symbols
SMAL; VRL2; BCYM3; CMT2C; SPSMA; TRP12; VROAC; HMSN2C; OTRPC4; SSQTL1
NCBI Protein Information
transient receptor potential cation channel subfamily V member 4
UniProt Protein Name
Transient receptor potential cation channel subfamily V member 4
UniProt Gene Name
TRPV4
UniProt Synonym Gene Names
VRL2; VROAC; TrpV4; OTRPC4; TRP12; VRL-2; VR-OAC
UniProt Entry Name
TRPV4_HUMAN
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Product Notes
The TRPV4 trpv4 (Catalog #AAA197958) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRPV4 antibody - middle region reacts with Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TRPV4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the TRPV4 trpv4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: RVDEVNWSHW NQNLGIINED PGKNETYQYY GFSHTVGRLR RDRWSSVVPR. It is sometimes possible for the material contained within the vial of "TRPV4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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