WB (Western Blot)
(Western blot analysis of extracts from PC-3 cells, using TRPV6 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit TRPV6 Polyclonal Antibody | anti-TRPV6 antibody
TRPV6 Antibody
Reactivity
Human, Mouse, RatPredicted: Horse, Rabbit, Dog
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
TRPV6, Antibody; TRPV6 Antibody; anti-TRPV6 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Horse, Rabbit, Dog
Predicted: Horse, Rabbit, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
TRPV6 Antibody detects endogenous levels of TRPV6.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
725
Applicable Applications for anti-TRPV6 antibody
WB (Western Blot)
Immunogen
A synthesized peptide derived from human TRPV6.
Tissue Specificity
Expressed at high levels in the gastrointestinal tract, including esophagus, stomach, duodenum, jejunum, ileum and colon, and in pancreas, placenta, prostate and salivary gland. Expressed at moderate levels in liver, kidney and testis. Expressed in trophoblasts of placenta villus trees (at protein level)(PubMed:23612980). Expressed in locally advanced prostate cancer, metastatic and androgen-insensitive prostatic lesions but not detected in healthy prostate tissue and benign prostatic hyperplasia.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Plasma Membrane
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from PC-3 cells, using TRPV6 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from PC-3 cells, using TRPV6 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Rat brain, using TRPV6 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 70 kDa.)
WB (Western Blot)
(Western blot analysis of extracts from Rat brain, using TRPV6 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 70 kDa.)
Related Product Information for anti-TRPV6 antibody
Description: Calcium selective cation channel that mediates Ca2+ uptake in various tissues, including the intestine (PubMed:11097838, PubMed:11278579, PubMed:11248124 PubMed:15184369, PubMed:23612980, PubMed:29258289). Important for normal Ca2+ ion homeostasis in the body, including bone and skin (By similarity). The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification (PubMed:15184369). Inactivation includes both a rapid Ca2+-dependent and a slower Ca2+-calmodulin-dependent mechanism; the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg2+ in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating.
Post Translational Modifications: Glycosylated. Phosphorylation at Tyr-201 by SRC leads to an increased calcium influx through the channel. Probably dephosphorylated at this site by PTPN1 (By similarity). Phosphorylation by PRKCA at the calmodulin binding site delays channel inactivation (PubMed:11248124).
Subunit Structure: Homotetramer (PubMed:29258289). Probably forms also heterotetramers with TRPV5. Interacts with TRPV5. Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane. Interacts with BSPRY (By similarity). Interacts with TCAF1 and TCAF2 isoform 2 (PubMed:25559186). Interacts with calmodulin (PubMed:11248124).
Similarity: Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV6 sub-subfamily.
Post Translational Modifications: Glycosylated. Phosphorylation at Tyr-201 by SRC leads to an increased calcium influx through the channel. Probably dephosphorylated at this site by PTPN1 (By similarity). Phosphorylation by PRKCA at the calmodulin binding site delays channel inactivation (PubMed:11248124).
Subunit Structure: Homotetramer (PubMed:29258289). Probably forms also heterotetramers with TRPV5. Interacts with TRPV5. Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane. Interacts with BSPRY (By similarity). Interacts with TCAF1 and TCAF2 isoform 2 (PubMed:25559186). Interacts with calmodulin (PubMed:11248124).
Similarity: Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV6 sub-subfamily.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
Observed: 83 kDa
Predicted: 88 kDa
Predicted: 88 kDa
NCBI Official Full Name
Transient receptor potential cation channel subfamily V member 6
UniProt Protein Name
Transient receptor potential cation channel subfamily V member 6
UniProt Gene Name
TRPV6
UniProt Synonym Gene Names
ECAC2; TrpV6; CaT-L; CaT1; ECaC2
UniProt Entry Name
TRPV6_HUMAN
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Product Notes
The TRPV6 trpv6 (Catalog #AAA328751) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRPV6 Antibody reacts with Human, Mouse, Rat Predicted: Horse, Rabbit, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's TRPV6 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the TRPV6 trpv6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRPV6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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