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FCM (Flow Cytometry)
(Flow cytometric analysis of HUVEC cells with UBE1C antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green).)
Rabbit UBE1C Polyclonal Antibody | anti-UBE1C antibody
UBE1C Antibody
Gene Names
UBA3; NAE2; UBE1C; hUBA3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
UBE1C; Polyclonal Antibody; UBE1C Antibody; DKFZp566J164 antibody; EC 6.3.2. antibody; hUba3 antibody; MGC22384 antibody; NEDD8 activating enzyme E1 catalytic subunit antibody; NEDD8 activating enzyme E1C antibody; Nedd8 activating enzyme hUba3 antibody; NEDD8-activating enzyme E1 catalytic subunit antibody; NEDD8-activating enzyme E1C antibody; uba3 antibody; UBA3 ubiquitin activating enzyme E1 homolog antibody; UBA3_HUMAN antibody; UBE1C antibody; Ubiquitin activating enzyme 3 antibody; Ubiquitin activating enzyme E1C antibody; Ubiquitin-activating enzyme 3 antibody; Ubiquitin-activating enzyme E1C antibody; Ubiquitin-like modifier-activating enzyme 3 antibody; anti-UBE1C antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-UBE1C antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of HUVEC cells with UBE1C antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green).)
FCM (Flow Cytometry)
(Flow cytometric analysis of HUVEC cells with UBE1C antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green).)
ICC (Immunocytochemistry)
(ICC staining UBE1C in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining UBE1C in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining UBE1C in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining UBE1C in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining UBE1C in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining UBE1C in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UBE1C antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UBE1C antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-UBE1C antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-UBE1C antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-UBE1C antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-UBE1C antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of UBE1C on human liver tissue lysate using anti-UBE1C antibody at 1/200 dilution.)
WB (Western Blot)
(Western blot analysis of UBE1C on human liver tissue lysate using anti-UBE1C antibody at 1/200 dilution.)
Related Product Information for anti-UBE1C antibody
Ubiquitin is an abundant, highly conserved protein found in all eukaryotic cells either free or covalently attached to cellular proteins. The primary function of ubiquitin in mammalian systems is to clear abnormal, foreign and improperly folded proteins by targeting them for proteosome degradation. UBA3 (ubiquitin-like modifier activating enzyme 3), also known as NEDD8-activating enzyme E1 catalytic subunit or UBE1C (ubiquitin-activating enzyme E1C), is a 463 amino acid protein belonging to the ubiquitin-activating E1 family and UBA3 subfamily. Ubiquitously expressed, UBA3 acts as an activator to NEDD8, a ubiquitin-like protein, thus regulating cell division, signaling and embryogenesis. UBA3 exists as two isoforms due to alternative splicing events.
Product Categories/Family for anti-UBE1C antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
463
NCBI Official Full Name
NEDD8-activating enzyme E1 catalytic subunit
NCBI Official Synonym Full Names
ubiquitin-like modifier activating enzyme 3
NCBI Official Symbol
UBA3
NCBI Official Synonym Symbols
NAE2; UBE1C; hUBA3
NCBI Protein Information
NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1C; Nedd8-activating enzyme hUba3; ubiquitin-activating enzyme 3; NEDD8-activating enzyme E1 subunit 2; ubiquitin-like modifier-activating enzyme 3; UBA3, ubiquitin-activating enzyme E
UniProt Protein Name
NEDD8-activating enzyme E1 catalytic subunit
UniProt Gene Name
UBA3
UniProt Synonym Gene Names
UBE1C; Ubiquitin-activating enzyme 3
UniProt Entry Name
UBA3_HUMAN
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Product Notes
The UBE1C uba3 (Catalog #AAA29884) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBE1C Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's UBE1C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the UBE1C uba3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE1C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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