WB (Western Blot)
(Western blot analysis of HEK293 cell lysates using rabbit pAb to ubiquitin, AAA76744, dilution 1:5,000 in green and mouse mAb to beta-Tubulin in red, used as a loading control. [1] protein standard (red), [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10uM for 16 hours. The smear detected above the 200kDa standard represents accumulations of ubiquitinated proteins in the Lc treated cells.)
Rabbit Ubiquitin Polyclonal Antibody | anti-UBB antibody
Ubiquitin
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry
Synonyms
Ubiquitin, Antibody; Ubiquitin; anti-UBB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Form/Format
Liquid. Supplied as an aliquot of serum plus 5mM NaN3
Applicable Applications for anti-UBB antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry)
Immunogen
Purified ubiquitin conjugated with glutaraldehyde to KLH
Preparation and Storage
Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for sixmonths without detectable loss of activity. The antibody can be stored at 2 degree - 8 degree C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of HEK293 cell lysates using rabbit pAb to ubiquitin, AAA76744, dilution 1:5,000 in green and mouse mAb to beta-Tubulin in red, used as a loading control. [1] protein standard (red), [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10uM for 16 hours. The smear detected above the 200kDa standard represents accumulations of ubiquitinated proteins in the Lc treated cells.)
WB (Western Blot)
(Western blot analysis of HEK293 cell lysates using rabbit pAb to ubiquitin, AAA76744, dilution 1:5,000 in green and mouse mAb to beta-Tubulin in red, used as a loading control. [1] protein standard (red), [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10uM for 16 hours. The smear detected above the 200kDa standard represents accumulations of ubiquitinated proteins in the Lc treated cells.)
IF (Immunofluorescence)
(Immunofluorescent analysis of HeLa cells stained with rabbit pAb to ubiquitin, AAA76744, dilution 1:1,000 in red, and costained with chicken pAb to vimentin in green. The blue is DAPI staining of nuclear DNA. [A] Control HeLa cells maintained in normal medium, [B] HeLa cells treated with 10uM of the proteasome inhibitor lactacystin (Lc) for 24 hours. Proteasomal inhibition leads to formation of strongly ubiquitin positive cytoplasmic inclusions.)
IF (Immunofluorescence)
(Immunofluorescent analysis of HeLa cells stained with rabbit pAb to ubiquitin, AAA76744, dilution 1:1,000 in red, and costained with chicken pAb to vimentin in green. The blue is DAPI staining of nuclear DNA. [A] Control HeLa cells maintained in normal medium, [B] HeLa cells treated with 10uM of the proteasome inhibitor lactacystin (Lc) for 24 hours. Proteasomal inhibition leads to formation of strongly ubiquitin positive cytoplasmic inclusions.)
Related Product Information for anti-UBB antibody
Ubiquitin is a globular 76 amino acid protein of about 8.5kDa molecular weight which was discovered by biochemical isolation from bovine thymus tissues. The protein was found to be highly conserved in amino acid sequence and was detectable in apparently every cell and tissue type, and, being apparently ubiquitously expressed, became known as ubiquitin. Subsequent work showed that ubiquitin has an important role in the targeting of proteins for proteolytic degradation, but has other important functions.
It was made against purified bovine blood derived ubiquitin coupled to keyhole limpet hemocyanin with glutaraldehyde. The antibody is relatively insensitive to formalin fixation and so can be used on paraffin embedded fixed histological sections of human brain for studies of Alzheimer's and other neurodegenerative diseases.
It was made against purified bovine blood derived ubiquitin coupled to keyhole limpet hemocyanin with glutaraldehyde. The antibody is relatively insensitive to formalin fixation and so can be used on paraffin embedded fixed histological sections of human brain for studies of Alzheimer's and other neurodegenerative diseases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
229
NCBI Official Full Name
Polyubiquitin-B
NCBI Official Synonym Full Names
ubiquitin B
NCBI Official Symbol
UBB
NCBI Protein Information
polyubiquitin-B; polyubiquitin B
UniProt Protein Name
Polyubiquitin-B
UniProt Gene Name
UBB
UniProt Entry Name
UBB_HUMAN
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Product Notes
The UBB ubb (Catalog #AAA76744) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ubiquitin reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ubiquitin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the UBB ubb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ubiquitin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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