FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of H-PBMC cells using anti-uPA Receptor antibody.Overlay histogram showing H-PBMC cells stained with A00993-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-uPA Receptor antibody.Overlay histogram showing H-PBMC cells stained with A00993-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-uPA Receptor Antibody for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit uPA Receptor/PLAUR Polyclonal Antibody | anti-PLAUR antibody
Anti-uPA Receptor/PLAUR Antibody
Gene Names
PLAUR; CD87; UPAR; URKR; U-PAR
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
uPA Receptor/PLAUR, Antibody; Anti-uPA Receptor/PLAUR Antibody; Urokinase plasminogen activator surface receptor; U-PAR; Monocyte activation antigen Mo3; uPAR; CD87; Plaur; MO3; UPAR; Plasminogen activator, urokinase receptor; anti-PLAUR antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
281
Applicable Applications for anti-PLAUR antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence of human uPA Receptor/PLAUR (TKSGCNHPDLDVQYRS).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-F and ICC.
Preparation and Storage
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.
Avoid repeated freezing and thawing.
Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of H-PBMC cells using anti-uPA Receptor antibody.Overlay histogram showing H-PBMC cells stained with A00993-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-uPA Receptor antibody.Overlay histogram showing H-PBMC cells stained with A00993-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-uPA Receptor Antibody for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of H-PBMC cells using anti-uPA Receptor antibody.Overlay histogram showing H-PBMC cells stained with A00993-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-uPA Receptor antibody.Overlay histogram showing H-PBMC cells stained with A00993-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-uPA Receptor Antibody for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of uPA Receptor using anti-uPA Receptor antibody.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysate,Lane 2: human U20S whole cell lysate,Lane 3: human A431 whole cell lysate,Lane 4: human Hela whole cell lysate,Lane 5: human A549 whole cell lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-uPA Receptor antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for uPA Receptor at approximately 39KD. The expected band size for uPA Receptor is at 37KD.)
WB (Western Blot)
(Figure 1. Western blot analysis of uPA Receptor using anti-uPA Receptor antibody.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysate,Lane 2: human U20S whole cell lysate,Lane 3: human A431 whole cell lysate,Lane 4: human Hela whole cell lysate,Lane 5: human A549 whole cell lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-uPA Receptor antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for uPA Receptor at approximately 39KD. The expected band size for uPA Receptor is at 37KD.)
Related Product Information for anti-PLAUR antibody
PLAUR(PLASMINOGEN ACTIVATOR RECEPTOR, UROKINASE-TYPE), also known as UPAR or CD87, is multidomain glycoprotein tethered to the cell membrane with a glycosylphosphotidylinositol (GPI) anchor. PLAUR consists of three different domains of the Ly-6/uPAR/alpha-neurotoxin family. PLAUR is originally identified as a saturable binding site for urokinase on the cell surface. And the gene plays an importan trole in many normal as well as pathologic processes. The PLAUR gene is localized to 19q13.31. PLAUR is a part of the plasminogen activation system, which in the healthy body is involved in tissue reorganization events such as mammary gland involution and wound healing. PLAUR binds urokinase and thus restricts plasminogen activation to the immediate vicinity of the cell membrane. Thus it seems to be an important player in the regulation of this process. In human coronary artery vascular smooth muscle cells, UPA stimulates cell migration via a UPAR signaling complex containing TYK2 and phosphatidylinositol 3-kinase.
References
1. Borglum, A., Anette, B., Roldan, A. L., Blasi, F., Bolund, L., Kruse, T. A. Assignment of the gene for urokinase-type plasminogen activator receptor to chromosome 19q13. 2. Borglum, A. D., Byskov, A., Ragno, P., Roldan, A. L., Tripputi, P., Cassani, G., Dano, K., Blasi, F., Bolund, L., Kruse, T. A. Assignment of the urokinase-type plasminogen activator receptor gene (PLAUR) to chromosome 19q13.1-q13.2. Am. J. Hum. Genet. 50: 492-497, 1992. 3. Llinas P, Le Du MH, Gårdsvoll H, et al. (May 2005). "Crystal structure of the human urokinase plasminogen activator receptor bound to an antagonist peptide".
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
urokinase plasminogen activator surface receptor isoform 2
NCBI Official Synonym Full Names
plasminogen activator, urokinase receptor
NCBI Official Symbol
PLAUR
NCBI Official Synonym Symbols
CD87; UPAR; URKR; U-PAR
NCBI Protein Information
urokinase plasminogen activator surface receptor
UniProt Protein Name
Urokinase plasminogen activator surface receptor
UniProt Gene Name
PLAUR
UniProt Synonym Gene Names
MO3; UPAR; U-PAR; uPAR
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Product Notes
The PLAUR plaur (Catalog #AAA124914) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-uPA Receptor/PLAUR Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's uPA Receptor/PLAUR can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PLAUR plaur for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "uPA Receptor/PLAUR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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