WB (Western Blot)
(Detection of human USP36/DUB1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP36/DUB1 antibody AAA211238 (lot AAA211238-2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human USP36/DUB1 Polyclonal Antibody | anti-USP36/DUB1 antibody
Rabbit anti-USP36/DUB1 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
USP36/DUB1, Antibody; Rabbit anti-USP36/DUB1 Antibody, Affinity Purified; ubiquitin specific protease 36; ubiquitin-specific-processing protease 36; ubiquitin thioesterase 36; ubiquitin carboxyl-terminal hydrolase 36; deubiquitinating enzyme 36; DUB1; Ubiquitin carboxyl-terminal hydrolase 36; ubiquitin specific peptidase 36; anti-USP36/DUB1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-USP36/DUB1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1073 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human USP36/DUB1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP36/DUB1 antibody AAA211238 (lot AAA211238-2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human USP36/DUB1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP36/DUB1 antibody AAA211238 (lot AAA211238-2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human USP36/DUB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP36/DUB1 antibody AAA211238 (lot AAA211238-2) used for IP at 6 ug per reaction. USP36/DUB1 was also immunoprecipitated by rabbit anti-USP36/DUB1 antibody BL4146. For blotting immunoprecipitated USP36/DUB1, AAA211238 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human USP36/DUB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP36/DUB1 antibody AAA211238 (lot AAA211238-2) used for IP at 6 ug per reaction. USP36/DUB1 was also immunoprecipitated by rabbit anti-USP36/DUB1 antibody BL4146. For blotting immunoprecipitated USP36/DUB1, AAA211238 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human USP36/DUB1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-USP36/DUB1(Cat. No. AAA211238 Lot 2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human USP36/DUB1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-USP36/DUB1(Cat. No. AAA211238 Lot 2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-USP36/DUB1 antibody
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 36 (USP36) is a member of this family.
Product Categories/Family for anti-USP36/DUB1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The USP36/DUB1 usp36 (Catalog #AAA211238) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-USP36/DUB1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP36/DUB1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the USP36/DUB1 usp36 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP36/DUB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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