IF (Immunofluorescence)
(Immunofluorescent analysis of Vasopressin V2 Receptor staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Rabbit Vasopressin V2 Receptor Polyclonal Antibody | anti-AVPR2 antibody
Anti-Vasopressin V2 Receptor Antibody
Gene Names
AVPR2; DI1; DIR; NDI; V2R; ADHR; DIR3
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
Vasopressin V2 Receptor, Antibody; Anti-Vasopressin V2 Receptor Antibody; ADHR; DIR; DIR3; V2R; Vasopressin V2 receptor; AVPR V2; Antidiuretic hormone receptor; Renal-type arginine vasopressin receptor; anti-AVPR2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Vasopressin V2 Receptor protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Sequence Length
371
Applicable Applications for anti-AVPR2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC).
Application Notes
WB: 1/500-1/1000
IF/ICC: 1/100-1/500
IF/ICC: 1/100-1/500
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Vasopressin V2 Receptor. The exact sequence is proprietary.
Preparation and Storage
Upon receipt aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of Vasopressin V2 Receptor staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IF (Immunofluorescence)
(Immunofluorescent analysis of Vasopressin V2 Receptor staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
WB (Western Blot)
(Western blot analysis of Vasopressin V2 Receptor expression in DLD (A), mouse muscle (B), rat kidney (C) whole cell lysates.(Predicted band size: 40 kD; Observed band size: 45 kD))
WB (Western Blot)
(Western blot analysis of Vasopressin V2 Receptor expression in DLD (A), mouse muscle (B), rat kidney (C) whole cell lysates.(Predicted band size: 40 kD; Observed band size: 45 kD))
Related Product Information for anti-AVPR2 antibody
Rabbit polyclonal antibody to Vasopressin V2 Receptor
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Vasopressin V2 receptor
NCBI Official Synonym Full Names
arginine vasopressin receptor 2
NCBI Official Symbol
AVPR2
NCBI Official Synonym Symbols
DI1; DIR; NDI; V2R; ADHR; DIR3
NCBI Protein Information
vasopressin V2 receptor
UniProt Protein Name
Vasopressin V2 receptor
UniProt Gene Name
AVPR2
UniProt Synonym Gene Names
ADHR; DIR; DIR3; V2R; V2R
UniProt Entry Name
V2R_HUMAN
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Product Notes
The AVPR2 avpr2 (Catalog #AAA27813) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Vasopressin V2 Receptor Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Vasopressin V2 Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500-1/1000 IF/ICC: 1/100-1/500. Researchers should empirically determine the suitability of the AVPR2 avpr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vasopressin V2 Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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