FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 293 cells using VE Cadherin Antibody (CDH5) (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human VE Cadherin Polyclonal Antibody | anti-CDH5 antibody
VE Cadherin Antibody (CDH5) (N-term)
Gene Names
CDH5; 7B4; CD144
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
VE Cadherin, Antibody; VE Cadherin Antibody (CDH5) (N-term); Cadherin-5; 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CD144; CDH5; anti-CDH5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This VE Cadherin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-134 amino acids from the N-terminal region of human VE Cadherin.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.43 (varies by lot)
Sequence Positions
106-134
Sequence Length
784
Applicable Applications for anti-CDH5 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 293 cells using VE Cadherin Antibody (CDH5) (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 293 cells using VE Cadherin Antibody (CDH5) (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of anti-CDH5 Pab in 293 cell line lysates (35ug/lane). CDH5(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-CDH5 Pab in 293 cell line lysates (35ug/lane). CDH5(arrow) was detected using the purified Pab.)
Related Product Information for anti-CDH5 antibody
CDH5 is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions.
Product Categories/Family for anti-CDH5 antibody
References
Breviario F.,Arterioscler. Thromb. Vasc. Biol. 15:1229-1239(1995)
Ali J.,Microcirculation 4:267-277(1997)
Suzuki S.,Cell Regul. 2:261-270(1991)
Ali J.,Microcirculation 4:267-277(1997)
Suzuki S.,Cell Regul. 2:261-270(1991)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
87528
NCBI Official Full Name
cadherin-5 preproprotein
NCBI Official Synonym Full Names
cadherin 5, type 2 (vascular endothelium)
NCBI Official Symbol
CDH5
NCBI Official Synonym Symbols
7B4; CD144
NCBI Protein Information
cadherin-5
UniProt Protein Name
Cadherin-5
UniProt Gene Name
CDH5
UniProt Synonym Gene Names
VE-cadherin
UniProt Entry Name
CADH5_HUMAN
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Product Notes
The CDH5 cdh5 (Catalog #AAA284584) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 106-134. The VE Cadherin Antibody (CDH5) (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VE Cadherin can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CDH5 cdh5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VE Cadherin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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