IHC (Immunohistochemisry)
(Immunohistochemical staining of paraffin-embedded RT2 xenograft using Wilms Tumor 1 antibody at a dilution of 1:500)
Rabbit Wilms Tumor 1 Polyclonal Antibody | anti-WT1 antibody
Wilms Tumor 1 antibody
Gene Names
WT1; GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Wilms Tumor 1 antibody was purified by antigen-affinity chromatography
Synonyms
Wilms Tumor 1, Antibody; Wilms Tumor 1 antibody; Polyclonal Wilms Tumor 1; Anti-Wilms Tumor 1; Wilms tumor 1; Wilms Tumor 1; WAGR; GUD; WT33; AWT1; Wilms Tumor -1; NPHS4; WIT-2; amino-terminal domain of EWS|last three Zinc fingers of the DNA-binding domain of WT1; Wilms tumor protein; WT1; anti-WT1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Wilms Tumor 1 antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.21 mg/ml (varies by lot)
Sequence Length
30
Applicable Applications for anti-WT1 antibody
WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence)
Biological Significance
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms.
Cross-Reactivity
Human, Rat
Immunogen
Wilms Tumor 1 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 209 and 449 of Wilms Tumor 1
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IHC (Immunohistochemisry)
(Immunohistochemical staining of paraffin-embedded RT2 xenograft using Wilms Tumor 1 antibody at a dilution of 1:500)
IHC (Immunohistochemisry)
(Immunohistochemical staining of paraffin-embedded RT2 xenograft using Wilms Tumor 1 antibody at a dilution of 1:500)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: Molt-4; B: Raji) using a 10 % SDS PAGE gel and Wilms Tumor 1 antibody at a dilution of 1:1000)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: Molt-4; B: Raji) using a 10 % SDS PAGE gel and Wilms Tumor 1 antibody at a dilution of 1:1000)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using WT1 antibody at 1:200 dilution.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using WT1 antibody at 1:200 dilution.)
Related Product Information for anti-WT1 antibody
Affinity purified Rabbit polyclonal Wilms Tumor 1 antibody
Product Categories/Family for anti-WT1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
56 kDa
NCBI Official Full Name
Wilms tumor 1, partial
NCBI Official Synonym Full Names
Wilms tumor 1
NCBI Official Symbol
WT1
NCBI Official Synonym Symbols
GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
NCBI Protein Information
Wilms tumor protein
UniProt Protein Name
Wilms tumor protein
UniProt Gene Name
WT1
UniProt Entry Name
WT1_HUMAN
Similar Products
Product Notes
The WT1 wt1 (Catalog #AAA224209) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Wilms Tumor 1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the WT1 wt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wilms Tumor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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