Rabbit Wilms Tumor 1 (WT1) Monoclonal Antibody | anti-WT1 antibody

Wilms Tumor 1 (WT1) (Wilms Tumor & Mesothelial Marker)

Cat. Num. AAA214560

• Gene Names: WT1; GUD; AWT1; WAGR; WT33; NPHS4; WIT-2
• Reactivity: Human. Others-not known
• Applications: Immunohistochemistry
• Synonyms: Wilms Tumor 1 (WT1), Antibody; Wilms Tumor 1 (WT1) (Wilms Tumor & Mesothelial Marker); WT1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1; anti-WT1 antibody

• Host: Rabbit
• Reactivity: Human. Others-not known
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: WT1/1434R
• Specificity: Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm’s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm’s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm’s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the mesotheliomas.
• Form/Format: 200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 497

• Applicable Applications for anti-WT1 antibody: IHC (Immunohistochemistry)
• Immunogen: Recombinant human full-length WT1 protein
• Cellular Localization: Nuclear and Cytoplasmic
• Positive Control: K562 cells. Wilm’s Tumor, mesothelioma or fetal kidney.
• Supplied As: 200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Preparation and Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis of PurifiedWilm’s Tumor Rabbit Recombinant Monoclonal Antibody (WT1/1434R).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded Rat Testis stained with Wilm’s Tumor Rabbit Recombinant Monoclonal Antibody (WT1/1434R).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Human Mesothelioma stained with Wilm’s Tumor Rabbit Recombinant Monoclonal Antibody (WT1/1434R).)

References

Rauscher FJ. The WT1 Wilms tumor gene product: A developmentally regulated transcription factor in the kidney that functions as a tumor suppressor. FASEB J 1993; 7:896

NCBI and Uniprot Product Information

• NCBI GI #: 65507714
• NCBI GeneID: 7490
• NCBI Accession #: NP_000369.3
• NCBI GenBank Nucleotide #: NM_000378.4
• UniProt Accession #: P19544; Q15881; Q16256; Q16575; Q4VXV4; Q4VXV5; Q4VXV6; Q8IYZ5; A8K6S1; B3KSA5
• Molecular Weight: 47-55kDa
• NCBI Official Full Name: Wilms tumor protein isoform A
• NCBI Official Synonym Full Names: Wilms tumor 1
• NCBI Official Symbol: WT1
• NCBI Official Synonym Symbols: GUD; AWT1; WAGR; WT33; NPHS4; WIT-2
• NCBI Protein Information: Wilms tumor protein
• UniProt Protein Name: Wilms tumor protein
• UniProt Gene Name: WT1