Rabbit WT1 Polyclonal Antibody | anti-WT1 antibody

WT1 Antibody

Cat. Num. AAA306230

• Gene Names: WT1; GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: WT1, Antibody; WT1 Antibody; GUD; AWT1; WAGR; WT33; NPHS4; anti-WT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total WT1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 497

• Applicable Applications for anti-WT1 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human WT1.
• SDS-PAGE MW: 49kD
• Target Name: WT1
• Preparation and Storage: Store at -20°C

IF (Immunofluorescence)

(Immunofluorescence analysis of HeLa cell using WT1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human liver damage using WT1 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded rat lung using WT1 antibody at dilution of 1:500 (400x lens).)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded human kidney cancer using WT1 antibody at dilution of 1:500 (400x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines using WT1 at 1:1000 dilution.)

Related Product Information for anti-WT1 antibody

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

Product Categories/Family for anti-WT1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 65507714
• NCBI GeneID: 7490
• NCBI Accession #: NP_000369.3
• NCBI GenBank Nucleotide #: NM_000378.4
• UniProt Accession #: P19544; Q15881; Q16256; Q16575; Q4VXV4; Q4VXV5; Q4VXV6; Q8IYZ5; A8K6S1; B3KSA5
• NCBI Official Full Name: Wilms tumor protein isoform A
• NCBI Official Synonym Full Names: Wilms tumor 1
• NCBI Official Symbol: WT1
• NCBI Official Synonym Symbols: GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
• NCBI Protein Information: Wilms tumor protein
• UniProt Protein Name: Wilms tumor protein
• UniProt Gene Name: WT1
• UniProt Entry Name: WT1_HUMAN