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WB (Western Blot)
(Anti-WT1 Antibody (Center E361) at 1:1000 dilution + Mouse testis lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit WT1 Polyclonal Antibody | anti-WT1 antibody
WT1 Antibody (Center E361)
Gene Names
WT1; GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
Reactivity
Human, MousePredicted Reactivity: Xenopus, Pig, Rat
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
WT1, Antibody; WT1 Antibody (Center E361); Wilms tumor protein; WT33; WT1; anti-WT1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted Reactivity: Xenopus, Pig, Rat
Predicted Reactivity: Xenopus, Pig, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This WT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the Central region of human WT1.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
346-375
Sequence Length
375
Applicable Applications for anti-WT1 antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA, IF (Immunofluorescence)
Function
Transcription factor that plays an important role in cellular development and cell survival(PubMed: ). Recognizes and binds to the DNA sequence5'-GCG(T/G)GGGCG-3' (PubMed: , PubMed:17716689, PubMed: ). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenicrole in tumor formation. Function may be isoform-specific: isoforms lacking the KTSmotif may act as transcription factors (PubMed: ). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing(PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA(PubMed: ).
Cellular Location
Nucleus. Nucleus, nucleolus. Cytoplasm. Note=Isoforms lacking the KTS motif have a diffuse nuclear location (PubMed:15520190) Shuttles between nucleus and cytoplasm. {ECO:0000250, ECO:0000269|PubMed:15520190} Isoform 4: Nucleus, nucleoplasm
Tissue Location
Expressed in the kidney and a subset of hematopoietic cells
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Anti-WT1 Antibody (Center E361) at 1:1000 dilution + Mouse testis lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-WT1 Antibody (Center E361) at 1:1000 dilution + Mouse testis lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of WT1 Antibody (Center E361) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of WT1 Antibody (Center E361) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohistochemisry)
(WT1 Antibody (Center E361) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WT1 Antibody (Center E361) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(WT1 Antibody (Center E361) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WT1 Antibody (Center E361) for immunohistochemistry. Clinical relevance has not been evaluated.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA284950 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA284950, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA284950 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA284950, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
WB (Western Blot)
(Anti-WT1 Antibody (Center E361) at 1:1000 dilution + Mouse testis lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 49 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-WT1 Antibody (Center E361) at 1:1000 dilution + Mouse testis lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 49 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-WT1 antibody
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq].
Product Categories/Family for anti-WT1 antibody
References
Sitaram, R.T., et al. Br. J. Cancer 103(8):1255-1262(2010)
Dohi, S., et al. Anticancer Res. 30(8):3187-3192(2010)
Rocquain, J., et al. BMC Cancer 10, 401 (2010) :
Wagner, K.D., et al. J. Cell. Sci. 116 (PT 9), 1653-1658 (2003) :
Mitsuya, K., et al. Hum. Mol. Genet. 6(13):2243-2246(1997)
Dohi, S., et al. Anticancer Res. 30(8):3187-3192(2010)
Rocquain, J., et al. BMC Cancer 10, 401 (2010) :
Wagner, K.D., et al. J. Cell. Sci. 116 (PT 9), 1653-1658 (2003) :
Mitsuya, K., et al. Hum. Mol. Genet. 6(13):2243-2246(1997)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Wilms tumor protein isoform A
NCBI Official Synonym Full Names
Wilms tumor 1
NCBI Official Symbol
WT1
NCBI Official Synonym Symbols
GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
NCBI Protein Information
Wilms tumor protein
UniProt Protein Name
Wilms tumor protein
UniProt Gene Name
WT1
UniProt Entry Name
WT1_HUMAN
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Product Notes
The WT1 wt1 (Catalog #AAA284950) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 346-375. The WT1 Antibody (Center E361) reacts with Human, Mouse Predicted Reactivity: Xenopus, Pig, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's WT1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the WT1 wt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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