WB (Western Blot)
(Mouse Xaf1 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Xaf1 antibody detected the Xaf1 protein (arrow).)
Rabbit anti-Mouse Xaf1 Polyclonal Antibody | anti-Xaf1 antibody
Mouse Xaf1 Antibody (Center)
Gene Names
Xaf1; Fbox39
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
Xaf1, Antibody; Mouse Xaf1 Antibody (Center); XIAP-associated factor 1; BIRC4-binding protein; Xaf1; Birc4bp; Xiapaf1; anti-Xaf1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This Mouse Xaf1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-194 amino acids from the Central region of mouse Xaf1.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
166-194
Sequence Length
273
Applicable Applications for anti-Xaf1 antibody
ELISA, WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Mouse Xaf1 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Xaf1 antibody detected the Xaf1 protein (arrow).)
WB (Western Blot)
(Mouse Xaf1 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Xaf1 antibody detected the Xaf1 protein (arrow).)
Related Product Information for anti-Xaf1 antibody
Xaf1 seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells (By similarity).
Product Categories/Family for anti-Xaf1 antibody
References
Bai, Y., et al. J. Biol. Chem. 283(11):6832-6842(2008)
Qiao, L., et al. Tumour Biol. 29(2):122-129(2008)
Wang, X., et al. Neurobiol. Dis. 16(1):179-189(2004)
Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003)
Qiao, L., et al. Tumour Biol. 29(2):122-129(2008)
Wang, X., et al. Neurobiol. Dis. 16(1):179-189(2004)
Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31117
NCBI Official Full Name
XIAP-associated factor 1 isoform 1
NCBI Official Synonym Full Names
XIAP associated factor 1
NCBI Official Symbol
Xaf1
NCBI Official Synonym Symbols
Fbox39
NCBI Protein Information
XIAP-associated factor 1
UniProt Protein Name
XIAP-associated factor 1
UniProt Gene Name
Xaf1
UniProt Synonym Gene Names
Birc4bp; Xiapaf1
UniProt Entry Name
XAF1_MOUSE
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Product Notes
The Xaf1 xaf1 (Catalog #AAA289240) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 166-194. The Mouse Xaf1 Antibody (Center) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Xaf1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Xaf1 xaf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Xaf1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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