IP (Immunoprecipitation)
(Detection of human YAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-YAP1 antibody AAA211840 used for IP at 10 ug/mg lysate. YAP1 was also immunoprecipitated by rabbit anti-YAP1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated YAP1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human YAP1 Polyclonal Antibody | anti-YAP1 antibody
Rabbit anti-YAP1 Antibody, Affinity Purified
Gene Names
YAP1; YAP; YKI; COB1; YAP2; YAP65
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
YAP1, Antibody; Rabbit anti-YAP1 Antibody, Affinity Purified; YAP; yorkie homolog; YKI; yes-associated protein YAP65 homolog; yes-associated protein 2; yes-associated protein 1; YAP2; transcriptional coactivator YAP1; protein yorkie homolog; COB1; 65 kDa Yes-associated protein; YAP65; Transcriptional coactivator YAP1; Yes associated protein 1; anti-YAP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-YAP1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 425 and 475
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human YAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-YAP1 antibody AAA211840 used for IP at 10 ug/mg lysate. YAP1 was also immunoprecipitated by rabbit anti-YAP1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated YAP1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human YAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-YAP1 antibody AAA211840 used for IP at 10 ug/mg lysate. YAP1 was also immunoprecipitated by rabbit anti-YAP1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated YAP1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human YAP1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-YAP1 (Cat. No. AAA211840 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human YAP1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-YAP1 (Cat. No. AAA211840 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-YAP1 antibody
Yes-associated protein 1 (YAP1) is the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction [taken from NCBI GeneID:10413].
Product Categories/Family for anti-YAP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,942 Da
NCBI Official Full Name
yorkie homolog isoform 2
NCBI Official Synonym Full Names
Yes-associated protein 1
NCBI Official Symbol
YAP1
NCBI Official Synonym Symbols
YAP; YKI; COB1; YAP2; YAP65
NCBI Protein Information
transcriptional coactivator YAP1; yorkie homolog; protein yorkie homolog; yes-associated protein 2; 65 kDa Yes-associated protein; yes-associated protein YAP65 homolog
UniProt Protein Name
Yorkie homolog
UniProt Gene Name
YAP1
UniProt Synonym Gene Names
YAP65; YAP65
UniProt Entry Name
YAP1_HUMAN
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Product Notes
The YAP1 yap1 (Catalog #AAA211840) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-YAP1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's YAP1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the YAP1 yap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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