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IP (Immunoprecipitation)
(Immunoprecipitation of YTHDC2 in 200 ug extracts from 293T cells using 0.5 ug [KD Validated] YTHDC2 Rabbit pAb (AAA283183). Western blot analysis was performed using [KD Validated] YTHDC2 Rabbit pAb (AAA283183) at 1:1000 dilution.)
Rabbit anti-Human YTHDC2 Polyclonal Antibody | anti-YTHDC2 antibody
[KD Validated] YTHDC2 Rabbit pAb
Reactivity
Human
Applications
ELISA, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Affinity purification
Synonyms
YTHDC2, Antibody; [KD Validated] YTHDC2 Rabbit pAb; CAHL; hYTHDC2; YTHDC2; anti-YTHDC2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Sequence
MPVRYFIMKSSNLRNLEISQQKGIWSTTPSNERKLNRAFWESSIVYLVFSVQGSGHFQGFSRMSSEIGREKSQDWGSAGLGGVFKVEWIRKESLPFQFAHHLLNPWNDNKKVQISRDGQELEPLVGEQLLQLWERLPLGEKNTTD
Applicable Applications for anti-YTHDC2 antibody
ELISA, IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Cross Reactivity
Human, Mouse, Rat
Immunogen
Recombinant Protein corresponding to a sequence within amino acids 1286-1430 of human YTHDC2(NP_073739.3).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
IP (Immunoprecipitation)
(Immunoprecipitation of YTHDC2 in 200 ug extracts from 293T cells using 0.5 ug [KD Validated] YTHDC2 Rabbit pAb (AAA283183). Western blot analysis was performed using [KD Validated] YTHDC2 Rabbit pAb (AAA283183) at 1:1000 dilution.)
IP (Immunoprecipitation)
(Immunoprecipitation of YTHDC2 in 200 ug extracts from 293T cells using 0.5 ug [KD Validated] YTHDC2 Rabbit pAb (AAA283183). Western blot analysis was performed using [KD Validated] YTHDC2 Rabbit pAb (AAA283183) at 1:1000 dilution.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraffin-embedded Rat testis using [KD Validated] YTHDC2 Rabbit pAb(AAA283183) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraffin-embedded Rat testis using [KD Validated] YTHDC2 Rabbit pAb(AAA283183) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraffin-embedded Mouse testis using [KD Validated] YTHDC2 Rabbit pAb(AAA283183) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraffin-embedded Mouse testis using [KD Validated] YTHDC2 Rabbit pAb(AAA283183) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of lysates from wild type (WT) and YTHDC2 knockdown (KD) HeLa cells using [KD Validated] YTHDC2 Rabbit pAb (AAA283183) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of lysates from wild type (WT) and YTHDC2 knockdown (KD) HeLa cells using [KD Validated] YTHDC2 Rabbit pAb (AAA283183) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of various lysates using [KD Validated] YTHDC2 Rabbit pAb (AAA283183) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates / proteins: 25 ug per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of various lysates using [KD Validated] YTHDC2 Rabbit pAb (AAA283183) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates / proteins: 25 ug per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
Related Product Information for anti-YTHDC2 antibody
This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein binds to N6-methyladenosine, a common modified RNA nucleotide that is enriched in the stop codons and 3' UTRs of eukaryotic messenger RNAs. Binding of proteins to this modified nucleotide may regulate mRNA translation and stability. This gene may be associated with susceptibility to pancreatic cancer in human patients, and knockdown of this gene resulted in reduced proliferation in a human liver cancer cell line.
Product Categories/Family for anti-YTHDC2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 160kDa
Observed MW: 170kDa
Observed MW: 170kDa
UniProt Protein Name
Probable ATP-dependent RNA helicase YTHDC2
UniProt Gene Name
YTHDC2
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Product Notes
The YTHDC2 ythdc2 (Catalog #AAA283183) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The [KD Validated] YTHDC2 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's YTHDC2 can be used in a range of immunoassay formats including, but not limited to, ELISA, IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the YTHDC2 ythdc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MPVRYFIMKS SNLRNLEISQ QKGIWSTTPS NERKLNRAFW ESSIVYLVFS VQGSGHFQGF SRMSSEIGRE KSQDWGSAGL GGVFKVEWIR KESLPFQFAH HLLNPWNDNK KVQISRDGQE LEPLVGEQLL QLWERLPLGE KNTTD. It is sometimes possible for the material contained within the vial of "YTHDC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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