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product-image-AAA211015_WB13.jpg WB (Western Blot) (Detection of human ZBTB7/FBI-1 by western blot and immunoprecipitation. Samples: A) Whole cell lysate from HeLa (5, 15 and 50 ug), HEK293T (T; 50 ug) and NIH 3T3 (M; 50 ug) cells. B) Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody AAA211015 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP (B). ZBTB7/FBI-1 was also immunoprecipitated using rabbit antibodies and BL2686, which are to downstream epitopes on ZBTB7/FBI-1. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)

Rabbit anti-Human ZBTB7/FBI-1 Polyclonal Antibody | anti-ZBTB7/FBI-1 antibody

Rabbit anti-ZBTB7/FBI-1 Antibody, Affinity Purified

Gene Names
ZBTB7A; LRF; FBI1; FBI-1; ZBTB7; ZNF857A; pokemon
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ZBTB7/FBI-1, Antibody; Rabbit anti-ZBTB7/FBI-1 Antibody, Affinity Purified; zinc finger protein 857A; Pokemon 1; POZ and Krueppel erythroid myeloid ontogenic factor; TIP21; TTF-I-interacting peptide 21; pokemon; factor binding IST protein 1; ZBTB7; ZNF857A; zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcripts binding protein; FBI1; POK erythroid myeloid ontogenic factor; zinc finger and BTB domain-containing protein 7A; factor that binds to inducer of short transcripts protein 1; FBI-1; HIV-1 1st-binding protein 1; HIV-1 inducer of short transcripts binding protein; leukemia/lymphoma-related factor; LRF; lymphoma related factor; Zinc finger and BTB domain-containing protein 7A; zinc finger and BTB domain containing 7A; anti-ZBTB7/FBI-1 antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-ZBTB7/FBI-1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 534 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human ZBTB7/FBI-1 by western blot and immunoprecipitation. Samples: A) Whole cell lysate from HeLa (5, 15 and 50 ug), HEK293T (T; 50 ug) and NIH 3T3 (M; 50 ug) cells. B) Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody AAA211015 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP (B). ZBTB7/FBI-1 was also immunoprecipitated using rabbit antibodies and BL2686, which are to downstream epitopes on ZBTB7/FBI-1. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)

product-image-AAA211015_WB13.jpg WB (Western Blot) (Detection of human ZBTB7/FBI-1 by western blot and immunoprecipitation. Samples: A) Whole cell lysate from HeLa (5, 15 and 50 ug), HEK293T (T; 50 ug) and NIH 3T3 (M; 50 ug) cells. B) Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody AAA211015 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP (B). ZBTB7/FBI-1 was also immunoprecipitated using rabbit antibodies and BL2686, which are to downstream epitopes on ZBTB7/FBI-1. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)

IHC (Immunohistochemistry)

(Detection of human ZBTB7/FBI-1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ZBTB7/FBI-1(Cat. No. AAA211015 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)

product-image-AAA211015_IHC15.jpg IHC (Immunohistochemistry) (Detection of human ZBTB7/FBI-1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ZBTB7/FBI-1(Cat. No. AAA211015 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-ZBTB7/FBI-1 antibody
Zinc-finger and BTB domain-containing protein 7A (ZBTB7A) binds IST (inducer of short transcript) DNA elements in the HIV promoter to facilitate short transcript synthesis. ZBTB7A identified as LRF also interacts with the LAZ-3/BCL-6 oncoprotein. Because ZBTB7A also contains a POZ domain it may serve a function in chromatin remodeling.
Product Categories/Family for anti-ZBTB7/FBI-1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61,439 Da
NCBI Official Full Name
zinc finger and BTB domain-containing protein 7A
NCBI Official Synonym Full Names
zinc finger and BTB domain containing 7A
NCBI Official Symbol
ZBTB7A
NCBI Official Synonym Symbols
LRF; FBI1; FBI-1; ZBTB7; ZNF857A; pokemon
NCBI Protein Information
zinc finger and BTB domain-containing protein 7A; TIP21; lymphoma related factor; zinc finger protein 857A; HIV-1 1st-binding protein 1; TTF-I-interacting peptide 21; factor binding IST protein 1; leukemia/lymphoma-related factor; POK erythroid myeloid on
UniProt Protein Name
Zinc finger and BTB domain-containing protein 7A
UniProt Gene Name
ZBTB7A
UniProt Synonym Gene Names
FBI1; LRF; ZBTB7; ZNF857A; FBI-1; POK erythroid myeloid ontogenic factor; Pokemon; TIP21
UniProt Entry Name
ZBT7A_HUMAN

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Product Notes

The ZBTB7/FBI-1 zbtb7a (Catalog #AAA211015) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ZBTB7/FBI-1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZBTB7/FBI-1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ZBTB7/FBI-1 zbtb7a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZBTB7/FBI-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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