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WB (Western Blot)
(WB Suggested Anti-ZEB1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 293T cell lysate)
Rabbit ZEB1 Polyclonal Antibody | anti-ZEB1 antibody
ZEB1 antibody - N-terminal region
Gene Names
ZEB1; BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
Reactivity
Tested: HumanPredicted: Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Zebrafish.
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Synonyms
ZEB1, Antibody; ZEB1 antibody - N-terminal region; anti-ZEB1 antibody
Host
Rabbit
Reactivity
Tested: Human
Predicted: Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Zebrafish.
Predicted: Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Zebrafish.
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
0.5 mg/ml (varies by lot)
Sequence
Synthetic peptide located within the following region: NPRRNNVTNYNTVVETNSDSDDEDKLHIVEEESVTDAADCEGVPEDDLPT
Sequence Length
1124
Applicable Applications for anti-ZEB1 antibody
WB (Western Blot), IHC (Immunohistochemistry)
Homology
Cow: 100%; Dog: 100%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 100%; Rat: 93%; Zebrafish: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human ZEB1
Protein Size (# AA)
1124 amino acids
Protein Interactions
SUMO1; CDK6; GTF2A1; UBR4; SUMO2; SIRT1; SOX2; CTBP2; CTBP1; SMAD7; SMAD6; SMARCA4; SERPINH1; SMAD3; EP300; DRAP1; KAT5; SMAD2; SMAD1; CDH1;
Blocking Peptide
For anti-ZEB1 (MBS3200324) antibody is Catalog #
Preparation and Storage
For short term use, store at 2°C to 8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(WB Suggested Anti-ZEB1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 293T cell lysate)
WB (Western Blot)
(WB Suggested Anti-ZEB1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 293T cell lysate)
WB (Western Blot)
(Lanes:1. 45ug capan1 cell lysate2. 45 ug HPAF cell lysatePrimary Antibody Dilution:1:1000Secondary Antibody:Anti-Rabbit HRPSecondary Antibody Dilution:1:5000Gene Name:ZEB1Submitted by:Dr. Pankaj Singh, UNMC, Omaha, NE)
WB (Western Blot)
(Lanes:1. 45ug capan1 cell lysate2. 45 ug HPAF cell lysatePrimary Antibody Dilution:1:1000Secondary Antibody:Anti-Rabbit HRPSecondary Antibody Dilution:1:5000Gene Name:ZEB1Submitted by:Dr. Pankaj Singh, UNMC, Omaha, NE)
WB (Western Blot)
(Host: RabbitTarget Name: ZEB1Sample Tissue: Human 786-0 Whole CellAntibody Dilution: 1ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: ZEB1Sample Tissue: Human 786-0 Whole CellAntibody Dilution: 1ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: ZEB1Sample Tissue: Human 786-0 Whole CellAntibody Dilution: 1ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: ZEB1Sample Tissue: Human 786-0 Whole CellAntibody Dilution: 1ug/ml)
IHC (Immunohistochemistry)
(Sample Type :Human Capan1 cells (Pancreatic cancer cell line)Primary Antibody Dilution :1:300Secondary Antibody :Anti-rabbit-AlexaFluor-488Secondary Antibody Dilution :1:200Color/Signal Descriptions :ZEB: Green DAPI: BlueGene Name :ZEB1Submitted by :Dr. Pankaj Singh, UNMC, Omaha, NE)
IHC (Immunohistochemistry)
(Sample Type :Human Capan1 cells (Pancreatic cancer cell line)Primary Antibody Dilution :1:300Secondary Antibody :Anti-rabbit-AlexaFluor-488Secondary Antibody Dilution :1:200Color/Signal Descriptions :ZEB: Green DAPI: BlueGene Name :ZEB1Submitted by :Dr. Pankaj Singh, UNMC, Omaha, NE)
Related Product Information for anti-ZEB1 antibody
Target Description: ZEB1 is a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site.
Product Categories/Family for anti-ZEB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
124kDa
NCBI Official Full Name
zinc finger E-box-binding homeobox 1 isoform b
NCBI Official Synonym Full Names
zinc finger E-box binding homeobox 1
NCBI Official Symbol
ZEB1
NCBI Official Synonym Symbols
BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
NCBI Protein Information
zinc finger E-box-binding homeobox 1
UniProt Protein Name
Zinc finger E-box-binding homeobox 1
UniProt Gene Name
ZEB1
UniProt Synonym Gene Names
AREB6; TCF8; TCF-8
UniProt Entry Name
ZEB1_HUMAN
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Product Notes
The ZEB1 zeb1 (Catalog #AAA197126) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZEB1 antibody - N-terminal region reacts with Tested: Human Predicted: Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Zebrafish. and may cross-react with other species as described in the data sheet. AAA Biotech's ZEB1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the ZEB1 zeb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: NPRRNNVTNY NTVVETNSDS DDEDKLHIVE EESVTDAADC EGVPEDDLPT. It is sometimes possible for the material contained within the vial of "ZEB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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