WB (Western Blot)
(ZEB1 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (40 ?g) probed with ZEB1 antibody (1:1,000).)
Rabbit anti-Human ZEB1 Polyclonal Antibody | anti-ZEB1 antibody
ZEB1 antibody (pAb)
Gene Names
ZEB1; BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Affinity Purified
Synonyms
ZEB1, Antibody; ZEB1 antibody (pAb); BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1; anti-ZEB1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-ZEB1 antibody
WB (Western Blot), IP (Immunoprecipitation)
Immunogen
This ZEB1 antibody was raised against a peptide derived from the N-terminal region of human ZEB1.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(ZEB1 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (40 ?g) probed with ZEB1 antibody (1:1,000).)
WB (Western Blot)
(ZEB1 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (40 ?g) probed with ZEB1 antibody (1:1,000).)
WB (Western Blot)
(ZEB1 antibody (pAb) tested by Immunoprecipitation. 10 ul of ZEB1 antibody was used to immunoprecipitate ZEB1 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the ZEB1 antibody at a dilution of 1:1,000.)
WB (Western Blot)
(ZEB1 antibody (pAb) tested by Immunoprecipitation. 10 ul of ZEB1 antibody was used to immunoprecipitate ZEB1 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the ZEB1 antibody at a dilution of 1:1,000.)
Related Product Information for anti-ZEB1 antibody
Short Description: Our ZEB1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: ZEB1 (Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein Negative regulator of IL2, NIL-2-A, Transcription factor 8, TCF-8) is a POU domain DNA-binding transcriptional regulator that inhibits the expression of E-cadherin and other epithelial genes central to adhesion and epithelial polarity. ZEB1 induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. ZEB1 positively regulates neuronal differentiation and it represses RCOR1 transcription activation during neurogenesis. Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3).
Background: ZEB1 (Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein Negative regulator of IL2, NIL-2-A, Transcription factor 8, TCF-8) is a POU domain DNA-binding transcriptional regulator that inhibits the expression of E-cadherin and other epithelial genes central to adhesion and epithelial polarity. ZEB1 induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. ZEB1 positively regulates neuronal differentiation and it represses RCOR1 transcription activation during neurogenesis. Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3).
Product Categories/Family for anti-ZEB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Predicted: 124 kDa
Observed: 130 kDa
Observed: 130 kDa
NCBI Official Synonym Full Names
zinc finger E-box binding homeobox 1
NCBI Official Symbol
ZEB1
NCBI Official Synonym Symbols
BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
NCBI Protein Information
zinc finger E-box-binding homeobox 1
UniProt Protein Name
Zinc finger E-box-binding homeobox 1
UniProt Gene Name
ZEB1
UniProt Synonym Gene Names
AREB6; TCF8; TCF-8
UniProt Entry Name
ZEB1_HUMAN
Similar Products
Product Notes
The ZEB1 zeb1 (Catalog #AAA59981) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZEB1 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZEB1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the ZEB1 zeb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZEB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.