Rabbit anti-Human ZIC3 Polyclonal Antibody | anti-ZIC3 antibody

Anti-ZIC3 Antibody, Rabbit Polyclonal

Cat. Num. AAA254319

• Gene Names: ZIC3; HTX; HTX1; ZNF203; VACTERLX
• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: ZIC3, Antibody; Anti-ZIC3 Antibody, Rabbit Polyclonal; ZIC3 Antibody, Rabbit PAb, Antigen Affinity Purified; Zic family member 3; Anti-HTX Antibody; Anti-HTX1 Antibody; Anti-VACTERLX Antibody; Anti-ZNF203 Antibody; anti-ZIC3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ZIC3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ZIC3 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the center region of the Human ZIC3
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ZIC3, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-ZIC3 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:54 kDaObserved band size:54 kDa)

IHC (Immunohistochemisry)

(Immunochemical staining of human ZIC3 in human brain with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

IHC (Immunohiostchemistry)

(Immunochemical staining of human ZIC3 in human prostate with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

IF (Immunofluorescence)

(Immunofluorescence staining of ZIC3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human ZIC3 polyclonal antibody (dilution ratio 1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to cytoplasm and nucleus.)

Related Product Information for anti-ZIC3 antibody

ZIC3, or zic family member 3, is a molecule that regulates early embryonic patterning in vertebrates. ZIC3 has putative roles in a number of developmental signalling pathways that have distinct roles in establishing the left-right axis. In addition, ZIC3 is also considered as target of MiR-564, a tumor suppressor in human lung cancer. Variants in the ZIC3 gene are rare, but have demonstrated their profound clinical significance in X-linked heterotaxy, affecting in particular male patients with abnormal arrangement of thoracic and visceral organs.

NCBI and Uniprot Product Information

• NCBI GI #: 4507973
• NCBI GeneID: 7547
• NCBI Accession #: NP_003404.1
• NCBI GenBank Nucleotide #: NM_003413.3
• UniProt Accession #: O60481; Q14DE5; Q5JY75; B2CNW4
• Molecular Weight: 50,045 Da
• NCBI Official Full Name: zinc finger protein ZIC 3
• NCBI Official Synonym Full Names: Zic family member 3
• NCBI Official Symbol: ZIC3
• NCBI Official Synonym Symbols: HTX; HTX1; ZNF203; VACTERLX
• NCBI Protein Information: zinc finger protein ZIC 3; heterotaxy 1; zinc finger protein 203; zinc finger protein of the cerebellum 3; Zic family member 3 (odd-paired homolog, Drosophila)
• UniProt Protein Name: Zinc finger protein ZIC 3
• UniProt Gene Name: ZIC3
• UniProt Synonym Gene Names: ZNF203
• UniProt Entry Name: ZIC3_HUMAN