FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-ZIC3 antibody (AAA125713).Overlay histogram showing MCF-7 cells stained with AAA125713 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZIC3 Antibody (AAA125713, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit ZIC3 Polyclonal Antibody | anti-ZIC3 antibody
Anti-ZIC3 Antibody
Gene Names
ZIC3; HTX; HTX1; ZNF203; VACTERLX
Reactivity
Human, Mouse, Rat
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
ZIC3, Antibody; Anti-ZIC3 Antibody; ZIC3; ZNF203; Zinc finger protein ZIC 3; Zinc finger protein 203; Zinc finger protein of the cerebellum 3; Zic family member 3; anti-ZIC3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for Apolipoprotein ZIC3 detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Applicable Applications for anti-ZIC3 antibody
ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E Coli-derived human ZIC3 recombinant protein (Position: N113-N455).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-ZIC3 antibody (AAA125713).Overlay histogram showing MCF-7 cells stained with AAA125713 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZIC3 Antibody (AAA125713, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-ZIC3 antibody (AAA125713).Overlay histogram showing MCF-7 cells stained with AAA125713 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZIC3 Antibody (AAA125713, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of ZIC3 using anti-ZIC3 antibody (AAA125713).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ZIC3 antigen affinity purified polyclonal antibody (Catalog # AAA125713) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ZIC3 at approximately 56-60KD. The expected band size for ZIC3 is at 56-60KD.)
WB (Western Blot)
(Figure 1. Western blot analysis of ZIC3 using anti-ZIC3 antibody (AAA125713).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ZIC3 antigen affinity purified polyclonal antibody (Catalog # AAA125713) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ZIC3 at approximately 56-60KD. The expected band size for ZIC3 is at 56-60KD.)
Related Product Information for anti-ZIC3 antibody
ZIC3 is a member of the Zinc finger of the cerebellum (ZIC) protein family. This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs.
References
1. Aruga, J., Nagai, T., Tokuyama, T., Hayashizaki, Y., Okazaki, Y., Chapman, V. M., Mikoshiba, K. The mouse Zic gene family: homologues of the Drosophila pair-rule gene odd-paired. J. Biol. Chem. 271: 1043-1047, 1996.
2. Bedard, J. E. J., Purnell, J. D., Ware, S. M. Nuclear import and export signals are essential for proper cellular trafficking and function of ZIC3. Hum. Molec. Genet. 16: 187-198, 2007.
3. Burdine, R. D., Schier, A. F. Conserved and divergent mechanisms in left-right axis formation. Genes Dev. 14: 763-776, 2000.
2. Bedard, J. E. J., Purnell, J. D., Ware, S. M. Nuclear import and export signals are essential for proper cellular trafficking and function of ZIC3. Hum. Molec. Genet. 16: 187-198, 2007.
3. Burdine, R. D., Schier, A. F. Conserved and divergent mechanisms in left-right axis formation. Genes Dev. 14: 763-776, 2000.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,045 Da
NCBI Official Full Name
zinc finger protein ZIC 3
NCBI Official Synonym Full Names
Zic family member 3
NCBI Official Symbol
ZIC3
NCBI Official Synonym Symbols
HTX; HTX1; ZNF203; VACTERLX
NCBI Protein Information
zinc finger protein ZIC 3; heterotaxy 1; zinc finger protein 203; zinc finger protein of the cerebellum 3; Zic family member 3 (odd-paired homolog, Drosophila)
UniProt Protein Name
Zinc finger protein ZIC 3
UniProt Gene Name
ZIC3
UniProt Synonym Gene Names
ZNF203
UniProt Entry Name
ZIC3_HUMAN
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Product Notes
The ZIC3 zic3 (Catalog #AAA125713) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ZIC3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ZIC3 can be used in a range of immunoassay formats including, but not limited to, ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the ZIC3 zic3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZIC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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