FCM/FACS (Flow Cytometry)
(ZIC3 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human ZIC3 Polyclonal Antibody | anti-ZIC3 antibody
ZIC3 Antibody (Center)
Gene Names
ZIC3; HTX; HTX1; ZNF203; VACTERLX
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ZIC3, Antibody; ZIC3 Antibody (Center); Zinc finger protein ZIC 3; Zinc finger protein 203; Zinc finger protein of the cerebellum 3; ZIC3; ZNF203; anti-ZIC3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ZIC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-267 amino acids from the Central region of human ZIC3.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
239-267
Sequence Length
467
Applicable Applications for anti-ZIC3 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ZIC3 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ZIC3 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(ZIC3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZIC3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(ZIC3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZIC3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(ZIC3 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ZIC3 antibody detected the ZIC3 protein (arrow).)
WB (Western Blot)
(ZIC3 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ZIC3 antibody detected the ZIC3 protein (arrow).)
Related Product Information for anti-ZIC3 antibody
This gene encodes a member of the ZIC family of C2H2-type
zinc finger proteins. This nuclear protein probably functions as a
transcription factor in early stages of left-right body axis
formation. Mutations in this gene cause X-linked visceral
heterotaxy, which includes congenital heart disease and left-right
axis defects in organs.
zinc finger proteins. This nuclear protein probably functions as a
transcription factor in early stages of left-right body axis
formation. Mutations in this gene cause X-linked visceral
heterotaxy, which includes congenital heart disease and left-right
axis defects in organs.
Product Categories/Family for anti-ZIC3 antibody
References
Hatayama, M., et al. Hum. Mol. Genet. 17(22):3459-3473(2008)
Zhu, L., et al. Hum. Mutat. 29(1):99-105(2008)
Zhu, L., et al. Hum. Mol. Genet. 16(14):1649-1660(2007)
Chhin, B., et al. Hum. Mutat. 28(6):563-570(2007)
Bedard, J.E., et al. Hum. Mol. Genet. 16(2):187-198(2007)
Zhu, H., et al. Am. J. Med. Genet. A 116A (4), 414-415 (2003) :
Koyabu, Y., et al. J. Biol. Chem. 276(10):6889-6892(2001)
Klootwijk, R., et al. Hum. Mol. Genet. 9(11):1615-1622(2000)
Gebbia, M., et al. Nat. Genet. 17(3):305-308(1997)
Casey, B., et al. Nat. Genet. 5(4):403-407(1993)
Zhu, L., et al. Hum. Mutat. 29(1):99-105(2008)
Zhu, L., et al. Hum. Mol. Genet. 16(14):1649-1660(2007)
Chhin, B., et al. Hum. Mutat. 28(6):563-570(2007)
Bedard, J.E., et al. Hum. Mol. Genet. 16(2):187-198(2007)
Zhu, H., et al. Am. J. Med. Genet. A 116A (4), 414-415 (2003) :
Koyabu, Y., et al. J. Biol. Chem. 276(10):6889-6892(2001)
Klootwijk, R., et al. Hum. Mol. Genet. 9(11):1615-1622(2000)
Gebbia, M., et al. Nat. Genet. 17(3):305-308(1997)
Casey, B., et al. Nat. Genet. 5(4):403-407(1993)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50569
NCBI Official Full Name
zinc finger protein ZIC 3
NCBI Official Synonym Full Names
Zic family member 3
NCBI Official Symbol
ZIC3
NCBI Official Synonym Symbols
HTX; HTX1; ZNF203; VACTERLX
NCBI Protein Information
zinc finger protein ZIC 3
UniProt Protein Name
Zinc finger protein ZIC 3
UniProt Gene Name
ZIC3
UniProt Synonym Gene Names
ZNF203
UniProt Entry Name
ZIC3_HUMAN
Similar Products
Product Notes
The ZIC3 zic3 (Catalog #AAA288686) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 239-267. The ZIC3 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZIC3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the ZIC3 zic3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZIC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.