SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PP2Aalpha/PPP2R1A Complex Recombinant Protein | PP2Aalpha/PPP2R1A recombinant protein
PP2Aalpha/PPP2R1A Complex, Active
Applications
Western Blot
Synonyms
PP2Aalpha/PPP2R1A Complex; N/A; PP2Aalpha/PPP2R1A Complex, Active; PP2Aalpha: RP-C; PP2Ac; PP2CA; PPP2CA PPP2R1A: MRD36; PP2A-Aalpha; PP2AAALPHA; PR65A; PP2Aalpha/PPP2R1A recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 20mM MOPS, pH7.5, 300mM NaCl, 10mM Glutathione, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Applicable Applications for PP2Aalpha/PPP2R1A recombinant protein
WB (Western Blot), Phosphatase Assay
Tag
GST
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for PP2Aalpha/PPP2R1A recombinant protein
Recombinant full length human PP2Aa and human PPP2R1A were expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The PP2Aa gene accession number is NM_002715; PPP2R1A is NM_014225.
PP2Aalpha is also known as Protein phosphatase 2A active subunit. PP2A is one of the four major Ser/Thr phosphatases and is a human tumor suppressor that inhibits cellular transformation. PP2A alpha gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase-2A (1). Protein phosphatase 2A holoenzyme is a heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. PTP2A alpha can alter the substrate specificity of PP2A leading to enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity (2).
PP2Aalpha is also known as Protein phosphatase 2A active subunit. PP2A is one of the four major Ser/Thr phosphatases and is a human tumor suppressor that inhibits cellular transformation. PP2A alpha gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase-2A (1). Protein phosphatase 2A holoenzyme is a heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. PTP2A alpha can alter the substrate specificity of PP2A leading to enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity (2).
Product Categories/Family for PP2Aalpha/PPP2R1A recombinant protein
References
1. Van Hoof, C. et.al: Structure and chromosomal localization of the human gene of the phosphotyrosyl phosphatase activator (PTPA) of protein phosphatase 2A. Genomics 28: 261-272, 1995.
2. Chao, Y. et.al: Structure and mechanism of the phosphotyrosyl phosphatase activator. Molec. Cell 23: 535-546, 2006.
2. Chao, Y. et.al: Structure and mechanism of the phosphotyrosyl phosphatase activator. Molec. Cell 23: 535-546, 2006.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
PP2Aalpha -64kDa
PPP2R1A -91kDa
PPP2R1A -91kDa
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Product Notes
The PP2Aalpha/PPP2R1A (Catalog #AAA73806) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PP2Aalpha/PPP2R1A Complex can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Phosphatase Assay. Researchers should empirically determine the suitability of the PP2Aalpha/PPP2R1A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP2Aalpha/PPP2R1A Complex, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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