PRMT6 recombinant protein

Recombinant PRMT6 protein

Cat. Num. AAA59658

• Gene Names: PRMT6; HRMT1L6
• Purity: This recombinant protein is >= 85% pure by SDS-PAGE.
• Synonyms: PRMT6; N/A; Recombinant PRMT6 protein; HRMT1L6; PRMT6 recombinant protein

• Host: Baculovirus
• Purity/Purification: This recombinant protein is >= 85% pure by SDS-PAGE.
• Form/Format: Full length recombinant PRMT6 protein was expressed in Sf9 cells and is supplied in 25mM Hepes pH7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: Recombinant PRMT6 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions:60 ng/ul Calf Thymus Octamers were incubated with 30 ng/ul recombinant PRMT6 protein in reaction buffer including 50mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2mM MgCl2, 1mM TCEP, 9 nCi/ul 3H-SAM for 3 hours at room temperature. Activity was detected by fluorography.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

Application Data

(PRMT6 activity assay. 30 ng/ul Recombinant Histone H3.1 or 60 ng/ul Calf Thymus Octamer were incubated with 15 ng/ul PRMT6 (+) and 30 ng/ul PRMT6(++), respectively, in reaction buffer for 3 hours at room temperature. Activity was detected by fluorography.)

SDS-PAGE

(Recombinant PRMT6 protein gel. PRMT6 protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.)

Related Product Information for PRMT6 recombinant protein

Short Description: Recombinant PRMT6 (accession number NP_060607.2) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK tag with an observed molecular weight of 43.7 kDa. PRMT6 (Protein Arginine Methyltransferase 6, HRMT1L6, ANM6) is a type I arginine methyltransferase. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT6 preferentially methylates arginine residues present in GAR (glycine and arginine-rich) domains. PRMT6 asymmetrically dimethylates histone H3 at Arg2, which is mutually exclusive to methylation at lysine 4 (associated with transcriptional activation), and thus PRMT6 serves as a transcriptional repressor in this context. It can also symmetrically dimethylate H2A and H4 at Arg3 and serves as a coactivator of transcription in concert with nuclear receptors. Recombinant PRMT6 can be used to study enzyme kinetics and perform inhibitor screening assays.

Background: PRMT6 (Protein Arginine Methyltransferase 6, HRMT1L6, ANM6) is a type I arginine methyltransferase. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT6 preferentially methylates arginine residues present in GAR (glycine and arginine-rich) domains. PRMT6 asymmetrically dimethylates histone H3 at Arg2, which is mutually exclusive to methylation at lysine 4 (associated with transcriptional activation), and thus PRMT6 serves as a transcriptional repressor in this context. It can also symmetrically dimethylate H2A and H4 at Arg3 and serves as a coactivator of transcription in concert with nuclear receptors.

Product Categories/Family for PRMT6 recombinant protein

Recombinant Proteins; Histone Methyltransferase (HMT); Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 227908867
• NCBI GeneID: 55170
• NCBI Accession #: NP_060607.2
• NCBI GenBank Nucleotide #: NM_018137.2
• UniProt Accession #: Q96LA8; Q5T5Y5; Q6DKI4; Q9NVR8; A3KME1; B4DID8
• Molecular Weight: 32,509 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 6
• NCBI Official Synonym Full Names: protein arginine methyltransferase 6
• NCBI Official Symbol: PRMT6
• NCBI Official Synonym Symbols: HRMT1L6
• NCBI Protein Information: protein arginine N-methyltransferase 6; HMT1 hnRNP methyltransferase-like 6; histone-arginine N-methyltransferase PRMT6; heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6
• UniProt Protein Name: Protein arginine N-methyltransferase 6
• UniProt Gene Name: PRMT6
• UniProt Synonym Gene Names: HRMT1L6
• UniProt Entry Name: ANM6_HUMAN