IHC (Immunohistochemistry)
(Immunohistochemistry: Human carcinoma (FFPE) stained with Rabbit anti-TGF at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Rabbit GSK-3b (pY216) Antibody | anti-GSK-3b (pY216) antibody
Rabbit anti Phospho- GSK-3beta (pTyr 216)
Reactivity
Human, Rat, Mouse, Bovine, Chicken
Applications
ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Synonyms
GSK-3b (pY216); N/A; Rabbit anti Phospho- GSK-3beta (pTyr 216); Rabbit anti-Phospho-GSK3b (pY216); glycogen synthase kinase 3 beta (GSK3-b); anti-GSK-3b (pY216) antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Bovine, Chicken
Specificity
This antibody recognizes GSK-3beta (pY216) with a phosphorylated site at Tyrosine 216. It does not crossreact with non-phosphospecific peptide.
Purity/Purification
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Applicable Applications for anti-GSK-3b (pY216) antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.
Antigen Preparation
A synthetic peptide surrounding to the epitope -NVSYI- with a phosphorylation site at Tyr216 of human GSK3-beta protein. This sequence is identical among human, mouse, rat, bovine, dog and chicken species.
Positive Control
Kidney Tissue
Cellular Location
Cell Membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
IHC (Immunohistochemistry)
(Immunohistochemistry: Human carcinoma (FFPE) stained with Rabbit anti-TGF at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
IHC (Immunohistochemistry)
(Immunohistochemistry: Human carcinoma (FFPE) stained with Rabbit anti-TGF at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
WB (Western Blot)
(Western Blot: : The EGF stimulated A431 cell lysates (20 ug/lane) immunoblotted by Rabbit anti GSK-3beta (pY216) (Cat# AAA14095) antibody at 1:500. Observed a major immunoreactive band at molecular weight ~46kDa.)
WB (Western Blot)
(Western Blot: : The EGF stimulated A431 cell lysates (20 ug/lane) immunoblotted by Rabbit anti GSK-3beta (pY216) (Cat# AAA14095) antibody at 1:500. Observed a major immunoreactive band at molecular weight ~46kDa.)
Application Data
Application Data
Related Product Information for anti-GSK-3b (pY216) antibody
Glycogen Synthase Kinase-3beta (GSK-3beta) is a serine/threonine kinase that affects glycogen metabolism by phosphorylating and down-regulating the activity of muscle glycogen synthase. Growth factor (such as Insulin) binds to its receptor, recruits PKB/AKT kinase to phosphorylate GSK-3beta on Serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. In contrast, the phosphorylation of tyrosine 216 in the catalytic domain increase this kinase activity to promote cell death under the absence of growth factors. GSK-3beta is also involved in Wnt signaling pathway where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein, thus the GSK-3beta inhibitor is considered as a potential approach in Alzheimer's Disease (AD) treatment.
References
Alan P. Kozikowski, et al. Highly Potent and Specific GSK-3beta Inhibitors That Block Tau Phosphorylation and Decrease alpha-Synuclein Protein Expression in a Cellular Model of Parkinson's Disease. ChemMedChem, 2006, 1 (2), 256-266.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
46.0 kDa
NCBI Official Full Name
glycogen synthase kinase 3 beta
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Product Notes
The GSK-3b (pY216) (Catalog #AAA14095) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Phospho- GSK-3beta (pTyr 216) reacts with Human, Rat, Mouse, Bovine, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's GSK-3b (pY216) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Optimal dilutions should be determined by researchers for the specific applications. Researchers should empirically determine the suitability of the GSK-3b (pY216) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSK-3b (pY216), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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