IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using Phospho-MEF2C-S396 antibody. Blue: DAPI for nuclear staining.)
Rabbit anti-Human MEF2C-S396 Antibody | anti-MEF2C antibody
Phospho-MEF2C-S396 Polyclonal Antibody
Gene Names
MEF2C; DEL5q14.3; C5DELq14.3
Reactivity
Human
Applications
Immunofluorescence, Western Blot
Purity
Affinity Purification
Synonyms
MEF2C-S396; N/A; Phospho-MEF2C-S396 Polyclonal Antibody; Phospho-MEF2C-S396: DEL5q14.3; C5DELq14.3; anti-MEF2C antibody
Host
Rabbit
Reactivity
Human
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
473
Applicable Applications for anti-MEF2C antibody
IF (Immunofluorescence), WB (Western Blot)
Species
Human
Route
Synthetic Peptide
Immunogen
A phospho specific peptide corresponding to residues surrounding S396 of human MEF2C
Calculated Molecular Weight
51kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using Phospho-MEF2C-S396 antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using Phospho-MEF2C-S396 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts of K-562 and MCF-7 cells, using Phospho-MEF2C-S396 antibody at 1:1000 dilution. K562 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. MCF7 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
WB (Western Blot)
(Western blot analysis of extracts of K-562 and MCF-7 cells, using Phospho-MEF2C-S396 antibody at 1:1000 dilution. K562 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. MCF7 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Related Product Information for anti-MEF2C antibody
This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described.
Product Categories/Family for anti-MEF2C antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
473
NCBI Official Full Name
Myocyte-specific enhancer factor 2C
NCBI Official Synonym Full Names
myocyte enhancer factor 2C
NCBI Official Symbol
MEF2C
NCBI Official Synonym Symbols
DEL5q14.3; C5DELq14.3
NCBI Protein Information
myocyte-specific enhancer factor 2C; MADS box transcription enhancer factor 2, polypeptide C
UniProt Protein Name
Myocyte-specific enhancer factor 2C
UniProt Gene Name
MEF2C
UniProt Entry Name
MEF2C_HUMAN
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Product Notes
The MEF2C mef2c (Catalog #AAA36688) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-MEF2C-S396 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MEF2C-S396 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the MEF2C mef2c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEF2C-S396, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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