FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-SERT antibody 8B6 (AAA72257). Paraformaldehyde fixed HepG2 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of 8B6 (AAA72257-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Rabbit anti-Human SERT Antibody | anti-SERT antibody
Anti-SERT [8B6], Rabbit IgG
Gene Names
SLC6A4; HTT; 5HTT; OCD1; SERT; 5-HTT; SERT1; hSERT; 5-HTTLPR
Reactivity
Human
Applications
CRYS (Crystallization)
Purity
Protein A affinity purified
Synonyms
SERT; N/A; Anti-SERT [8B6], Rabbit IgG; Anti-SERT [8B6]; SLC6A4; Sodium-dependent serotonin transporter; 5HT transporter; Solute carrier family 6 member 4; HTT; anti-SERT antibody
Host
Rabbit
Reactivity
Human
Isotype
Rabbit IgG, Kappa
Clone Number
8B6
Specificity
The 8B6 Fab binds to extracellular loops 2 and 4 of hSERT, as shown by crystallography (Coleman et al, 2016).
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-SERT antibody
CRYS (Crystallization)
Immunogen
8B6 was produced by immunising SERT KO mice with residues 73-616 of wildtype human SERT. Hybridomas were generated using established protocols.
Origin Pub PMID
27049939
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-SERT antibody 8B6 (AAA72257). Paraformaldehyde fixed HepG2 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of 8B6 (AAA72257-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-SERT antibody 8B6 (AAA72257). Paraformaldehyde fixed HepG2 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of 8B6 (AAA72257-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cells with anti-SERT (AAA72257) 8B6 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells stained with the chimeric rabbit IgG version of 8B6 (AAA72257-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72257-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cells with anti-SERT (AAA72257) 8B6 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells stained with the chimeric rabbit IgG version of 8B6 (AAA72257-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72257-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-SERT antibody
The 8B6 Fab fragment was crystallised in complex with human SERT to aid structure determination (Coleman et al, 2016).
Product Categories/Family for anti-SERT antibody
References
Coleman et al. Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram J Vis Exp. 2016; (117): 54792. PMID:27929454
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
70,325 Da
NCBI Official Full Name
serotonin transporter, serotonin uptake site, 5-HT uptake site
NCBI Official Synonym Full Names
solute carrier family 6 (neurotransmitter transporter), member 4
NCBI Official Symbol
SLC6A4
NCBI Official Synonym Symbols
HTT; 5HTT; OCD1; SERT; 5-HTT; SERT1; hSERT; 5-HTTLPR
NCBI Protein Information
sodium-dependent serotonin transporter; 5HT transporter; serotonin transporter 1; 5-hydroxytryptamine transporter; Na+/Cl- dependent serotonin transporter; 5-hydroxytryptamine (serotonin) transporter; solute carrier family 6 (neurotransmitter transporter,
UniProt Protein Name
Sodium-dependent serotonin transporter
UniProt Gene Name
SLC6A4
UniProt Synonym Gene Names
HTT; SERT; 5HTT
UniProt Entry Name
SC6A4_HUMAN
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Product Notes
The SERT slc6a4 (Catalog #AAA72257) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SERT [8B6], Rabbit IgG reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SERT can be used in a range of immunoassay formats including, but not limited to, CRYS (Crystallization). Researchers should empirically determine the suitability of the SERT slc6a4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SERT, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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