IHC (Immunohistochemistry)
(Immunohistochemistry analysis of PP2A Catalytic alpha in paraffin-embedded rat colon tissue using PP2A Catalytic ? Rabbit pAb (AAA28190) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining)
Rabbit PPP2CA Antibody | anti-PPP2CA antibody
PPP2CA Polyclonal Antibody
IF/ICC: 1:50 - 1:200
IHC-P: 1:50 - 1:200
IP: 0.5 ug- 4ug antibody for 200 ug- 500 ug extracts of whole cells
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of PP2A Catalytic alpha in paraffin-embedded rat colon tissue using PP2A Catalytic ? Rabbit pAb (AAA28190) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of PP2A Catalytic alpha in paraffin-embedded rat colon tissue using PP2A Catalytic ? Rabbit pAb (AAA28190) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of PP2A Catalytic alpha in paraffin-embedded humancervical cancer tissue using PP2A Catalytic alpha Rabbit pAb (AAA28190) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of PP2A Catalytic alpha in paraffin-embedded humancervical cancer tissue using PP2A Catalytic alpha Rabbit pAb (AAA28190) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining)
IHC (Immunohistchemistry)
(Immunohistochemistry analysis of PP2A Catalytic alpha in paraffin-embedded mouse heart tissue using PP2A Catalytic α Rabbit pAb (AAA28190) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining)
IHC (Immunohistchemistry)
(Immunohistochemistry analysis of PP2A Catalytic alpha in paraffin-embedded mouse heart tissue using PP2A Catalytic α Rabbit pAb (AAA28190) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 500 ug extracts of Rat brain cells using 3 ug PP2A Catalytic alpha antibody (AAA28190). Western blot was performed from the immunoprecipitate using PP2A Catalytic alpha antibody (AAA28190) at a dilution of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 500 ug extracts of Rat brain cells using 3 ug PP2A Catalytic alpha antibody (AAA28190). Western blot was performed from the immunoprecipitate using PP2A Catalytic alpha antibody (AAA28190) at a dilution of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200 ug extracts of 293T cells using 3 ug PP2A Catalytic alpha antibody (AAA28190). Western blot was performed from the immunoprecipitate using PP2A Catalytic alpha antibody (AAA28190) at a dilution of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200 ug extracts of 293T cells using 3 ug PP2A Catalytic alpha antibody (AAA28190). Western blot was performed from the immunoprecipitate using PP2A Catalytic alpha antibody (AAA28190) at a dilution of 1:1000.)
WB (Western Blot)
(Western blot analysis of various lysates, using PP2A Catalytic alpha Rabbit pAb (AAA28190) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s)
WB (Western Blot)
(Western blot analysis of various lysates, using PP2A Catalytic alpha Rabbit pAb (AAA28190) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using PP2A Catalytic alpha Rabbit pAb (AAA28190) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using PP2A Catalytic alpha Rabbit pAb (AAA28190) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using PP2A Catalytic alpha Rabbit pAb (AAA28190) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using PP2A Catalytic alpha Rabbit pAb (AAA28190) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
Calculated MW: 36kDa
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Product Notes
The PPP2CA ppp2ca (Catalog #AAA28190) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPP2CA Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PPP2CA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA). WB: 1:1000 - 1:5000 IF/ICC: 1:50 - 1:200 IHC-P: 1:50 - 1:200 IP: 0.5 ug- 4ug antibody for 200 ug- 500 ug extracts of whole cells. Researchers should empirically determine the suitability of the PPP2CA ppp2ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP2CA, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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