Mouse anti-Human T-Cell Surface Glycoprotein CD3 Epsilon (CD3e) Monoclonal Antibody | anti-CD3e antibody

Monoclonal Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)

Cat. Num. AAA152656

• Gene Names: CD3E; T3E; TCRE; IMD18
• Reactivity: Human
• Applications: Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Affinity Chromatography
• Synonyms: T-Cell Surface Glycoprotein CD3 Epsilon (CD3e), Antibody; Monoclonal Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e); T-Cell Surface Glycoprotein CD3 Epsilon; T3E; TCRE; CD3-E Moleule,Epsilon(CD3-TCR Complex); T-cell surface antigen T3/Leu-4 epsilon chain; anti-CD3e antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone Number: C3
• Specificity: The antibody is a mouse monoclonal antibody raised against CD3e. It has been selected for its ability to recognize CD3e in immunohistochemical staining and western blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 1mg/mL (varies by lot)
• Sequence Positions: Met28~Asn185

• Applicable Applications for anti-CD3e antibody: IP (Immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Recombinant T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)
• Preparation and Storage: Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.; Stable for 12 months if correctly stored.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

WB (Western Blot)

(Western Blot; Sample: Jurkat cell lysate Primary Ab: 2 ug/ml Mouse Anti-human CD3e Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohiostchemistry)

(DAB staining on IHC-P;Sample: human Spleen TissuePrimary Ab: 10ug/ml Mouse Anti-human CD3e AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P; Sample: human Spleen Tissue; Primary Ab: 20 ug/ml Mouse Anti-human CD3e Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

Product Categories/Family for anti-CD3e antibody

Monoclonal Antibodies (Primary Abs); Monoclonal antibodies; Immune moleule

NCBI and Uniprot Product Information

• NCBI GI #: 1345708
• NCBI GeneID: 916
• UniProt Accession #: P07766; A8K997
• Molecular Weight: 207
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3e molecule, epsilon (CD3-TCR complex)
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: T3E; TCRE; IMD18
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Gene Name: CD3E
• UniProt Synonym Gene Names: T3E
• UniProt Entry Name: CD3E_HUMAN