RAC2 recombinant protein

RAC2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA258006

• Gene Names: RAC2; Gx; EN-7; HSPC022; p21-Rac2
• Purity: >94% as determined by SDS-PAGE
• Synonyms: RAC2; N/A; RAC2 Protein, Human, Recombinant (His Tag); Human RAC2 Protein (His Tag); EN-7 Protein, Human; Gx Protein, Human; HSPC022 Protein, Human; p21-Rac2 Protein, Human; ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2); RAC2 recombinant protein

• Host: E Coli
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 20% glycerol, pH 7.4, 5% Trehalose, 5% mannitol, 0.01% Tween-80
• Sequence: Met1-Cys189

• Source: Human
• Predicted N Terminal: Met
• Tag: His Tag
• Protein Construction: A DNA sequence encoding the human RAC2 (P15153)(Met 1-Cys 189) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: It is recommended that sterile water (400ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycle.; ; Stability: Samples are stable for up to twelve months from date of receipt at -70 °C.

SDS-PAGE

Related Product Information for RAC2 recombinant protein

Background: Ras-related C3 botulinum toxin substrate 2 (Rac2) is a small G-protein belonging to the Ras subfamily of the GTPase family. Rac2 acts as an "on/off" switch for signal transduction cascades and motilities. When GDP is attached to the small G-protein, the enzyme is inactivated. Release of the GDP and replace of the GTP cativate the GTPasee. Rac2 remains active until the GTP is hydrolyzed to GDP. Rac2 is a hematopoietic-specific Rho family GTPase implicated as an important constituent of the NADPH oxidase complex and shares 92% amino acid identity with the ubiquitously expressed Rac1. The small G-protein Rac2 regulates the rearrangements of actin and membrane necessary for Fcy receptor-mediated phagocytosis by macrophages. Activated Rac2 binds to the p21-binding domain of PAK1 and this binding provided a basis for microscopic methods to localize activation of these G proteins inside cells.

Product Categories/Family for RAC2 recombinant protein

TGF-beta Signaling

References

Adam D, et al. (2003) Cdc42, Rac1, and Rac2 Display Distinct Patterns of Activation during Phagocytosis.Mol Biol Cell. 15 (8): 3509-19.Walmsley MJ, et al. (2003) Critical Roles for Rac1 and Rac2 GTPases in B Cell Development and Signaling. Science. 302 (5644): 459-62.Holland M, et al. (2011) RAC2, AEP, and ICAM1 expression are associated with CNS disease in a mouse model of pre-B childhood acute lymphoblastic leukemia. Blood. 118 (3): 638-49.

NCBI and Uniprot Product Information

• NCBI GI #: 4506381
• NCBI GeneID: 5880
• NCBI Accession #: NP_002863.1
• NCBI GenBank Nucleotide #: NM_002872.4
• UniProt Accession #: P15153; Q9UDJ4; AAB22207
• Molecular Weight: Molecular Mass: The recombinant human RAC2 comprises 200 amino acids and has a predicted molecular mass of 22.6 kDa. It migrates as an approximately 25 kDa band in SDS-PAGE under reducing conditions.
• NCBI Official Full Name: ras-related C3 botulinum toxin substrate 2
• NCBI Official Synonym Full Names: ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
• NCBI Official Symbol: RAC2
• NCBI Official Synonym Symbols: Gx; EN-7; HSPC022; p21-Rac2
• NCBI Protein Information: ras-related C3 botulinum toxin substrate 2; small G protein; Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2)
• UniProt Protein Name: Ras-related C3 botulinum toxin substrate 2
• UniProt Gene Name: RAC2
• UniProt Entry Name: RAC2_HUMAN