Rat Alpha-2 Macroglobulin ELISA Kit | A2M elisa kit
Rat Alpha 2-Macroglobulin ELISA Kit
Gene Names
A2M; A2MD; CPAMD5; FWP007; S863-7; DKFZp779B086
Reactivity
Rat
Synonyms
Alpha-2 Macroglobulin; N/A; Rat Alpha 2-Macroglobulin ELISA Kit; Rat Alpha-2 Macroglobulin; A2M elisa kit
Reactivity
Rat
Samples
Immunoperoxidase Assay for Determination of Alpha 2-Macroglobulin in Rat Samples
Assay Type
Sandwich
Intended Uses
The Alpha 2-Macroglobulin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Alpha 2-Macroglobulin in biological samples of rats.
Preparation and Storage
Store at 4 degree C.
Related Product Information for A2M elisa kit
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Alpha 2-Macroglobulin present in samples reacts with the anti-Alpha 2-Macroglobulin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-A2M antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound A2M. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of A2M in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of A2M in the test sample. The quantity of A2M in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Background: Alpha 2-Macroglobulin (A2M) is a major protease inhibitor in serum and an acute phase protein which increases significantly in concentration in the rat as a result of inflammation. The major pathophysiological role for rat alpha 2-macroglobulin has yet to be conclusively defined. This kit is specific to the alpha 2- macroglobulin and will not cross react with the closely related alpha 1-macroglobulin.
Background: Alpha 2-Macroglobulin (A2M) is a major protease inhibitor in serum and an acute phase protein which increases significantly in concentration in the rat as a result of inflammation. The major pathophysiological role for rat alpha 2-macroglobulin has yet to be conclusively defined. This kit is specific to the alpha 2- macroglobulin and will not cross react with the closely related alpha 1-macroglobulin.
Product Categories/Family for A2M elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
163,291 Da
NCBI Official Full Name
alpha-2-macroglobulin
NCBI Official Synonym Full Names
alpha-2-macroglobulin
NCBI Official Symbol
A2M
NCBI Official Synonym Symbols
A2MD; CPAMD5; FWP007; S863-7; DKFZp779B086
NCBI Protein Information
alpha-2-macroglobulin; alpha-2-M; OTTHUMP00000196829; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
UniProt Protein Name
Alpha-2-macroglobulin
UniProt Gene Name
A2M
UniProt Synonym Gene Names
CPAMD5
UniProt Entry Name
A2MG_HUMAN
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Product Notes
The Rat A2M a2m (Catalog #AAA76934) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA76934 ELISA Kit recognizes Rat A2M. It is sometimes possible for the material contained within the vial of "Alpha-2 Macroglobulin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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