Application Data
(The OmniKine Rat IL-6 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat IL-6 proteins within the range of 39-5000 pg/ml.)
Rat IL-6 ELISA Kit | IL-6 elisa kit
IL-6 (Rat) OmniKine ELISA Kit
Gene Names
Il6; ILg6; Ifnb2
Reactivity
Rat
Synonyms
IL-6; N/A; IL-6 (Rat) OmniKine ELISA Kit; Interleukin-6; IL-6 elisa kit
Reactivity
Rat
Specificity
The OmniKine™ Rat IL-6 ELISA is capable of recognizing both recombinant and naturally produced Rat IL-6 proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.
Form/Format
12 x 8-Well Microstrips
Sequence Length
211
Assay Type
Quantitative Sandwich
Samples
Cell Lysates, Sera and Plasma
Sensitivity
39-5000 pg/ml
Detection Method
Colorimetric 450 nm
Subtype
None
Preparation and Storage
Store entire kit at 4 degree C until use.
Application Data
(The OmniKine Rat IL-6 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat IL-6 proteins within the range of 39-5000 pg/ml.)
Application Data
(The OmniKine Rat IL-6 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat IL-6 proteins within the range of 39-5000 pg/ml.)
Related Product Information for IL-6 elisa kit
Background: Rat IL-6 or Interleukin-6 is a 211 amino acid cytokine protein encoded by the Il6 gene located at locus 4q11 on chromosome 4. After initial synthesis, it undergoes proteolytic processing of the 24 residue signal sequence from the 187 residue IL-6 peptide, thus allowing for proper folding and maturation. The IL-6 superfamily member is a cytokine with a variety of biological functions. Rat IL-6 is known to be a potent inducer of the acute phase response, involved in the final differentiation of B-cells into Ig-secreting cells, involved in lymphocyte and monocyte differentiation, an inducer of myeloma and plasmacytoma growth, and inducer of nerve cell differentiation. The cytokine tends to act on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Moreover, IL-6 acts as a myokine since it is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Principle of the Assay: The OmniKine™ Rat IL-6 ELISA Kit contains the components necessary for quantitative determination of natural orrecombinant Rat IL-6 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) isbound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Rat IL-6 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of nonspecific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Principle of the Assay: The OmniKine™ Rat IL-6 ELISA Kit contains the components necessary for quantitative determination of natural orrecombinant Rat IL-6 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) isbound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Rat IL-6 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of nonspecific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,357 Da
NCBI Official Full Name
interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
Il6
NCBI Official Synonym Symbols
ILg6; Ifnb2
NCBI Protein Information
interleukin-6
UniProt Protein Name
Interleukin-6
UniProt Gene Name
Il6
UniProt Synonym Gene Names
Il-6; IL-6
Similar Products
Product Notes
The Rat IL-6 il6 (Catalog #AAA315698) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA315698 ELISA Kit recognizes Rat IL-6. It is sometimes possible for the material contained within the vial of "IL-6, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.