Application Data
(This assay recognizes both recombinant and natural Rat LIFR. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Rat LIFR/CD118 ELISA Kit | LIFR/CD118 elisa kit
Rat LIFR/CD118 ELISA Kit
Reactivity
Rat
Synonyms
LIFR/CD118; N/A; Rat LIFR/CD118 ELISA Kit; LIFR ELISA Kit, Rat; leukemia inhibitory factor receptor alpha; LIFR/CD118 elisa kit
Reactivity
Rat
Specificity
For the quantitative determination of Rat LIFR/CD118 concentration in serum.
Assay Type
Solid Phase Sandwich ELISA (quantitative)
Sample Types
Serum
Detection Range
46.88-3000pg/mL
Sensitivity
13pg/mL
Conjugation
HRP
Interference
Preparations of human LIFR, LTF and mouse LIFR, LTF at 50ng/mL in a mid-range recombinant rat LIFR control were assayed for interference. No significant interference was observed.
Materials Provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Application Data
(This assay recognizes both recombinant and natural Rat LIFR. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Application Data
(This assay recognizes both recombinant and natural Rat LIFR. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Related Product Information for LIFR/CD118 elisa kit
This LIFR ELISA Kit, Rat is an enzyme-linked immunosorbent assay for the quantitative measurement of Rat LIFR protein in Serum. It contains recombinant Rat LIFR, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Background: LIFR (leukemia inhibitory factor receptor) belongs to the family of cytokine receptors. LIFR forms a high-affinity receptor complex with gp130, which mediates the activity of LIF (leukemia inhibitory factor) and thus affects the differentiation, proliferation, and survival of a wide variety of cells in the adult and the embryo. Besides LIF, LIFR can also bind to and activate CNTF (ciliary neurotrophic factor) and CLC (Cardiotrophin Like Cytokine). Evidence showed that in the retina, LIFR activating LIF, CT-1, and Cardiotrophin Like Cytokine (CLC) are strongly upregulated in response to preconditioning with bright cyclic light leading to robust activation of signal transducer and activator of transcription-3 (STAT3) in a time-dependent manner. Further, blocking LIFR activation during preconditioning using a LIFR antagonist (LIF05) attenuated the induced STAT3 activation and also resulted in reduced preconditioning-induced protection of the retinal photoreceptors. These data demonstrate that LIFR and its ligands play an essential role in endogenous neuroprotective mechanisms triggered by preconditioning-induced stress. LIFR was newly found to be a suppressor of hepatocellular carcinoma (HCC), one of the world's top five causes of cancer-related deaths.
Background: LIFR (leukemia inhibitory factor receptor) belongs to the family of cytokine receptors. LIFR forms a high-affinity receptor complex with gp130, which mediates the activity of LIF (leukemia inhibitory factor) and thus affects the differentiation, proliferation, and survival of a wide variety of cells in the adult and the embryo. Besides LIF, LIFR can also bind to and activate CNTF (ciliary neurotrophic factor) and CLC (Cardiotrophin Like Cytokine). Evidence showed that in the retina, LIFR activating LIF, CT-1, and Cardiotrophin Like Cytokine (CLC) are strongly upregulated in response to preconditioning with bright cyclic light leading to robust activation of signal transducer and activator of transcription-3 (STAT3) in a time-dependent manner. Further, blocking LIFR activation during preconditioning using a LIFR antagonist (LIF05) attenuated the induced STAT3 activation and also resulted in reduced preconditioning-induced protection of the retinal photoreceptors. These data demonstrate that LIFR and its ligands play an essential role in endogenous neuroprotective mechanisms triggered by preconditioning-induced stress. LIFR was newly found to be a suppressor of hepatocellular carcinoma (HCC), one of the world's top five causes of cancer-related deaths.
References
Gearing, D.P. et al., 1991, EMBO J. 10 (10): 2839-2848.
Gearing, D.P. et al., 1992, New Biol. 4 (1): 61-65.Mosley, B. et al., 1996, J. Biol. Chem. 271 (51): 32635-32643.
Timmermann, A. et al., 2002, Eur. J. Biochem. 269 (11): 2716-2726.
Lass, A. et al., 2002, Fertil. Steril. 76 (6): 1091-1096.
Gearing, D.P. et al., 1992, New Biol. 4 (1): 61-65.Mosley, B. et al., 1996, J. Biol. Chem. 271 (51): 32635-32643.
Timmermann, A. et al., 2002, Eur. J. Biochem. 269 (11): 2716-2726.
Lass, A. et al., 2002, Fertil. Steril. 76 (6): 1091-1096.
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Product Notes
The Rat LIFR/CD118 (Catalog #AAA258503) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA258503 ELISA Kit recognizes Rat LIFR/CD118. It is sometimes possible for the material contained within the vial of "LIFR/CD118, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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