Rat SCF ELISA Kit | SCF elisa kit

SCF (Rat) OmniKine ELISA Kit

Cat. Num. AAA315703

• Gene Names: Kitlg; Mgf; SCF; Kitl
• Reactivity: Rat
• Synonyms: SCF; N/A; SCF (Rat) OmniKine ELISA Kit; Kit ligand; Hematopoietic growth factor KL; Mast cell growth factor; Stem cell factor; MGF; c-Kit ligand; Kitl; Mgf; SCF elisa kit

• Reactivity: Rat
• Specificity: The OmniKine Rat SCF ELISA is capable of recognizing both recombinant and naturally produced Rat SCF proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 10% cross reactivity.; Human: SCF; The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity or interference.; Murine: SCF; The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.; Human: G-CSF, GM-CSF, M-CSF, PDGF-AA, PDGF-AB, PDGF-BB, SCGFalpha, SCGFbeta; Murine: G-CSF, GM-CSF, M-CSF; Rat: GM-SCF, IL-1alpha, IL-1beta, IL-3beta, IL-6, IL-7
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 273
• Samples: Cell lysates, serum and plasma
• Assay Type: Quantitative Sandwich
• Sensitivity: 16-2000 pg/ml

• Preparation and Storage: Store entire kit at 4 degree C until use.

Application Data

(The OmniKine Rat SCF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat SCF proteins within the range of 16-2000 pg/ml.)

Related Product Information for SCF elisa kit

Principle of the Assay: The OmniKine™ Rat SCF ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Rat SCF concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Rat SCF while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.!!Background/Introduction: Rat SCF or Stem Cell Factor, also known as Kit Ligand or Mast Cell Growth Factor, is a 273 amino acid cytokine protein encoded by the Kitlg gene located at locus 7q13 on chromosome 7. After initial synthesis of the peptide, the 25 residue signal sequence is proteolytically removed, thus allowing for SCF to properly fold and mature. SCF, a homodimeric member of the SCF family, essentially stimulates the proliferation of mast cells. Also, it has the ability to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture as well as mediate cell-cell adhesion. These functions are performed through acting synergistically with other cytokines and interleukins. Acting in the early stages of hematopoiesis, a soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.

NCBI and Uniprot Product Information

• NCBI GI #: 33667068
• NCBI GeneID: 60427
• NCBI Accession #: NP_068615.1
• NCBI GenBank Nucleotide #: NM_021843.4
• UniProt Accession #: P21581; Q9QWZ4; Q9Z2E7
• Molecular Weight: 27,681 Da
• NCBI Official Full Name: kit ligand isoform 1
• NCBI Official Synonym Full Names: KIT ligand
• NCBI Official Symbol: Kitlg
• NCBI Official Synonym Symbols: Mgf; SCF; Kitl
• NCBI Protein Information: kit ligand
• UniProt Protein Name: Kit ligand
• UniProt Gene Name: Kitlg
• UniProt Synonym Gene Names: Kitl; Mgf; MGF; SCF; sKITLG