Rat TNFalpha ELISA Kit | TNFalpha elisa kit

Rat TNFalpha PicoKine Quick ELISA Kit

Cat. Num. AAA128061

• Gene Names: Tnf; Tnfa; RATTNF; TNF-alpha
• Reactivity: Rat
• Applications: ELISA
• Synonyms: TNFalpha; N/A; Rat TNFalpha PicoKine Quick ELISA Kit; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form; Tnf; Tnfa, Tnfsf2; TNFalpha elisa kit

• Reactivity: Rat
• Samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Assay Range: 15.6pg/ml-1,000pg/ml
• Sensitivity: <1pg/ml
• Capture antibody and Detection antibody: monoclonal antibody from mouse|polyclonal antibody from goat
• Immunogen: Expression system for standard: E coli; Immunogen sequence: L80-L235
• Anticoagulant: heparin or EDTA
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for TNFalpha elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 10 ng/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
  O.D.	0.010	0.031	0.062	0.132	0.203	0.555	0.884	1.838

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/ml)	14	96	280	13	98	306
  Standard deviation	0.57	7.39	20.44	0.78	7.54	24.78
  CV (%)	4.1%	7.7%	7.3%	6%	7.7%	8.1%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	14	14	14	14	14	0	0%
  Sample 2	96	93	92	101	95	3.5	3.6%
  Sample 3	280	258	274	306	279	17.28	6.1%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Standard Curve (Sample)

(Rat TNF alpha PicoKine ELISA Kit standard curve)

Related Product Information for TNFalpha elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for TNFalpha elisa kit

Quick ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 6981662
• NCBI GeneID: 24835
• NCBI Accession #: NP_036807.1
• NCBI GenBank Nucleotide #: NM_012675.3
• UniProt Accession #: P16599; Q6EE11; Q9JI26; Q9JI27
• Molecular Weight: 25,806 Da
• NCBI Official Full Name: tumor necrosis factor
• NCBI Official Synonym Full Names: tumor necrosis factor
• NCBI Official Symbol: Tnf
• NCBI Official Synonym Symbols: Tnfa; RATTNF; TNF-alpha
• NCBI Protein Information: tumor necrosis factor
• UniProt Protein Name: Tumor necrosis factor
• UniProt Gene Name: Tnf
• UniProt Synonym Gene Names: Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2
• UniProt Entry Name: TNFA_RAT