GST T1-1 active protein
GST T1-1, Recombinant Human
Purity
>= 95% as assessed by inspection on a Coomassie Blue-stained SDS-PAGE gel.
Synonyms
GST T1-1; N/A; GST T1-1, Recombinant Human; Recombinant Human GST T1-1; GST T1-1 active protein
Host
Recombinant 6x His-tagged protein expressed in E Coli
Purity/Purification
>= 95% as assessed by inspection on a Coomassie Blue-stained SDS-PAGE gel.
Concentration
1.95 mg/mL total protein using the Bradford protein assay with BSA as a standard. (varies by lot)
Sequence Length
240
Storage Buffer
50 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM DTT, 1 mM EDTA and 50% glycerol
Procedural Notes
This purified product exhibits moderate enzymatic activity for Cumene hydroperoxide, the substrate that is most commonly used to assess the glutathione peroxidase activity of soluble GSTs. However, as is suspected for GST activity analysis using synthetic and natural substrates, low concentrations (high dilutions) of the enzyme may result in lower activity values. In contrast, initial velocities are much higher for more concentrated levels of enzyme but the rate decreases rapidly. Therefore, activity towards GST substrates and these considerations provide guidance when assaying this product under low concentrations or when using substrates other than Cumene hydroperoxide
Specific Activity
8.8 Units/mg as assayed by the spectrophotometric determination of NADPH oxidation coupled to the glutathione peroxidase activity of GST T1-1 on cumene hydroperoxide (1.5 mM) in the presence of reduced glutathione (1 mM) in 100 mM NaPO4 (pH 7.0) at room temperature.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
At -20 degree C; AVOID MULTIPLE FREEZE-THAW CYCLES
References
Pemble S., et al., Biochem. J., 300 (Pt 1):271-276 (1994)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
~25 kDa
NCBI Official Full Name
glutathione transferase T1-1
NCBI Official Synonym Full Names
glutathione S-transferase theta 1
NCBI Official Symbol
GSTT1
NCBI Protein Information
glutathione S-transferase theta-1; GST class-theta-1; glutathione transferase T1-1
UniProt Protein Name
Glutathione S-transferase theta-1
UniProt Gene Name
GSTT1
UniProt Entry Name
GSTT1_HUMAN
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Product Notes
The GST T1-1 gstt1 (Catalog #AAA71852) is an Active Protein produced from Recombinant 6x His-tagged protein expressed in E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "GST T1-1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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