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▼Recombinant Proteins
Recombinant proteins are purified laboratory reagents created through genetic engineering for advanced biomedical research, such as immunology, neuroscience, cancer research, and more.
At AAA Biotech, also known as AAA Bio or AAABio, we provide high-quality recombinant proteins. Our various protein products are carefully tested for consistent and reliable performance. With our proteins, we offer stringent quality control, lot-to-lot consistency, and precise specificity to help you achieve accurate results in your research.
Whether you're conducting research on cell expansion, polarization, differentiation, or cell processing, we can offer the exact recombinant proteins you need in order to support your work.
Viewing 4850-4900 of 10000 product results
HBcAg, Recombinant Protein (Cat# AAA59088)
Full Name
HBcAg Protein
Applications
ELISA
Purity
>90% by SDS-PAGE
Pricing
Cytokeratin 8, CK8, Recombinant Protein (Cat# AAA59090)
Full Name
Keratin type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, KRT8, CK8
Applications
SDS-PAGE, ELISA
Purity
>95% by SDS-PAGE
Pricing
Cytokeratin 18, CK18, Recombinant Protein (Cat# AAA59092)
Full Name
Cytokeratin 18, CK18 Protein (Active)
Applications
SDS-PAGE, ELISA
Purity
> 95% by SDS-PAGE
Pricing
NSE, Recombinant Protein (Cat# AAA59093)
Full Name
NSE Protein (Active)
Applications
SDS-PAGE, ELISA
Purity
>95% by SDS-PAGE
Pricing
Dengue virus 4, Recombinant Protein (Cat# AAA58554)
Full Name
Dengue virus type 4
Applications
ELISA
Purity
~ 95% by SDS-PAGE
Interleukin 3 (IL-3) Recombinant, Recombinant Protein (Cat# AAA58300)
Full Name
Human IL-3, Recombinant
Gene Names
IL3; IL-3; MCGF
Reactivity
Human
Purity
>98% pure (RP-HPLC and SDS-PAGE). Purified by chromatographic techniques.
Product is sterile filtered.
Product is sterile filtered.
C-Reactive Protein (CRP), Recombinant, Recombinant Protein (Cat# AAA58340)
Full Name
Human CRP, Recombinant
Gene Names
CRP; PTX1; MGC88244; MGC149895
Purity
Phosphorylcholine affinity purification
Purity: Single band at 1ug/lane
Purity: Single band at 1ug/lane
Pricing
Troponin I, Recombinant Protein (Cat# AAA58393)
Full Name
Troponin I - Cardiac Recomb.
Applications
ELISA
Purity
>= 95% pure (SDS-PAGE Coomassie staining). Metal Affinity Chromatography
Pricing
SDS-PAGE
SDS-PAGE
Cytokeratin-18 (CK-18), Recombinant Protein (Cat# AAA58739)
Full Name
Cytokeratin-18 (CK-18)
Gene Names
KRT18; K18; CK-18; CYK18
Purity
>95% by SDS-PAGE
Pricing
Interleukin-13 (IL-13), Recombinant Protein (Cat# AAA58750)
Full Name
Interleukin-13 (IL-13)
Gene Names
IL13; P600; IL-13
Purity
>95% by SDS-PAGE
Pricing
SDS-PAGE
(Recombinant SUV420H2 activity assay Recombinant Nucleosomes (H3.1) in various amounts were incubated with SUV420H2 protein. Reaction products were run on SDS-PAGE gel and detected with anti-H4K20me2 or anti-H4K20me3. The activity of SUV420H2 with nucleosomes as substrate is better than with histone octamers as substrate.)
SUV420H2, Recombinant Protein (Cat# AAA60105)
Full Name
Recombinant SUV420H2 protein
Gene Names
SUV420H2; KMT5C
Pricing
Application Data
(Recombinant PTPN1 (1-321) activity assay. 1 mM pNPP was incubated with different concentrations of PTPN1 protein in 100 ul reaction system including 50 mM HEPES pH 7.5, 2 mM EDTA, 3 mM DTT, 100 mM NaCl at room temperature. The absorbance at 405 nm was continuously monitored for 30 minutes.)
PTPN1, Recombinant Protein (Cat# AAA60114)
Full Name
Recombinant PTPN1 (1-321) protein
Gene Names
PTPN1; PTP1B
Pricing
Application Data
(MALDI-TOF for Recombinant PHD1 / EGLN2 protein 3 uM HIF-1a peptide (biotin-DLDLEALAPYIPADDDFQL) was incubated with 300 nM PHD1 / EGLN2 protein in a 30 ul reaction containing 20 mM Tris-HCl pH 7.5, 5 mM KCl, 1.5 mM MgCl2, 1 mM DTT, 100 uM 2-oxoglutarate, 100 uM ascorbate and 50 uM (NH4)2Fe(SO4)2-6H20 for 2 hr. at 30 degree C. Single 3 uM HIF-1a peptide was used as a negative control. MALDI-TOF was used for detection.)
PHD1, Recombinant Protein (Cat# AAA60115)
Full Name
Recombinant PHD1 (EGLN2) protein
Gene Names
EGLN2; EIT6; PHD1; HPH-1; HPH-3; HIFPH1; HIF-PH1
Pricing
Application Data
(HTRF assay for IKKb activity 1 uM STK S3 substrate was incubated with different concentrations of IKKb protein in 10 ul reaction system containing 1×Enzymatic Buffer, 2 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.)
IKKb, Recombinant Protein (Cat# AAA60117)
Full Name
Recombinant IKKb protein
Gene Names
IKBKB; IKK2; IKKB; NFKBIKB; IKK-beta
Pricing
WB (Western Blot)
(Western Blot for activity detection of Mononucleosomes (H3.3) 2 ug Recombinant Mononucleosomes (H3.3) were incubated with 0 ug, 0.25 ug, 0.5 ug, 1 ug NSD2-SET in reaction buffer for 3 hours at room temperature, respectively. Western blot was used to detect the generation of reaction products (H3K36me2). Recombinant Histone Octamers were used as control substrates. The Western Blot result showed that nucleosomes were more suitable substrate for NSD2-SET than histone octamers.)
Mononucleosomes, Recombinant Protein (Cat# AAA60119)
Full Name
Recombinant Mononucleosomes (H3.3)
Pricing
WB (Western Blot)
(Western Blot analysis for Recombinant Mono-nucleosomes H3K36me3 (MLA) – biotin Recombinant Mononucleosomes H3K36me3 (MLA) - biotin (Lane 1) and unmodified nucleosomes (Lane 2) were detected with anti-H3K36me3 antibody and anti-H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3K36me3 (MLA) - biotin can be detected by H3K36me3 antibody.)
Mononucleosomes H3K36me3, Recombinant Protein (Cat# AAA60124)
Full Name
Recombinant Mononucleosomes H3K36me3 (MLA)-biotin
Gene Names
HIST1H3A; H3/A; H3FA
Pricing
WB (Western Blot)
(Western Blot analysis for Recombinant Mono-nucleosomes (H3K27ac) Recombinant Mononucleosomes (H3K27ac) (Lane 1) and unmodified nucleosomes (Lane 2) were detected with anti- H3K27ac antibody and anti-H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes (H3K27ac) can be detected by anti-H3K27ac antibody.)
Mononucleosomes H3K27ac, Recombinant Protein (Cat# AAA60125)
Full Name
Recombinant Mononucleosomes H3K27ac
Gene Names
HIST1H2AC; H2A/l; H2AFL; dJ221C16.4
Pricing
Application Data
(HTRF assay for RXR-a (RXRA) activity 1 ?M dsDNA oligos (DNA sequence: 5’-ATAGGTCACGGAGAGGTCAGC-3’) were incubated with different concentrations of protein in 10 ?l binding system containing 50 mM HEPESNaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ?l anti-DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
RXR-alpha (RXRA), Recombinant Protein (Cat# AAA60126)
Full Name
Recombinant RXR-alpha (RXRA) protein
Gene Names
RXRA; NR2B1
Pricing
Application Data
(HTRF assay for BTK activity 1 uM TK substrate was incubated with different concentrations of BTK protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The detection reagents containing anti-TK antibody and SA-XL665, diluted in 1X Detection Buffer, were added and incubated with the reactions for 30 min. All operations and reactions were performed at room temperature, and HTRF KinASE TK assay was used to detect the enzymatic activity.)
BTK, Recombinant Protein (Cat# AAA60127)
Full Name
Recombinant BTK protein
Gene Names
BTK; AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
Pricing
Application Data
(HTRF for YTHDC2 (1279-1429) activity Oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDC2 (1279-1429) protein in a reaction system for 1 hour, then DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
YTHDC2, Recombinant Protein (Cat# AAA60134)
Full Name
Recombinant YTHDC2 (1279-1429) protein
Pricing
Application Data
(HTRF for Recombinant YTHDF1 (380-533) protein activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF1 (380-533) protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
YTHDF1, Recombinant Protein (Cat# AAA60135)
Full Name
Recombinant YTHDF1 (380-533) protein
Gene Names
YTHDF1; C20orf21
Pricing
Application Data
(Recombinant Mononucleosomes (H2A.X) DNA gel. Recombinant Mononucleosomes (H2A.X) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: free 601 DNA. Lane 3: Intact nucleosomes. Intact nucleosomes migrate much higher than free 601 DNA. The agarose gel result shows that almost all of 601 DNA wrap histone octamers to form nucleosomes.)
Mononucleosomes, Recombinant Protein (Cat# AAA60147)
Full Name
Recombinant Mononucleosomes (H2A.X)
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Pricing
Application Data
(ALKBH3 dioxygenase activity 1 ug of ssDNA oligos (5’-AAAGCAG(1mA) ATTCGAAAAAGCGAAA-3’) was incubated with varying concentrations of ALKBH3 in a reaction system including 50 mM HEPES-NaOH pH 8.0, 50 uM Fe(NH4)2(SO4)2, 1 mM 2-oxoglutarate, 2 mM ascorbate and 1 mM TCEP for 30 min at 37 degree C. Then ssDNA oligos were annealed with equimolar of non-methylated complement strand followed by 1 ug EcoRI digestion for 45 min at 37 degree C. % reaction products were run on a 20% Native PAGE gel and stained by ethidium bromide.)
ALKBH3, Recombinant Protein (Cat# AAA60149)
Full Name
Recombinant ALKBH3 protein
Gene Names
ALKBH3; ABH3; PCA1; DEPC1; DEPC-1
Pricing
Application Data
(HTRF assay for FAK (409-698) activity 1 uM TK substrate was incubated with different concentrations of FAK protein and 100 uM ATP in the reaction buffer for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. TK antibody was used to detect the products. All the operations and reactions were performed at room temperature, and HTRF KinEASE TK assay was used to detect the enzymatic activity.)
FAK, Recombinant Protein (Cat# AAA60153)
Full Name
Recombinant FAK (409-698) protein
Gene Names
PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
Pricing
Application Data
(HTRF assay for recombinant AKT3 protein activity 1 uM STK S3 substrate was incubated with different concentrations AKT3 protein in a 10 ul reaction system for 1 hour. The 10 ul detection reagents were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature, and HTRF KinEASE STK assay was used to detect the enzymatic activity.)
AKT3, Recombinant Protein (Cat# AAA60156)
Full Name
Recombinant AKT3 protein
Gene Names
AKT3; PKBG; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
Pricing
Application Data
(HTRF assay for BRD2 (344-455), GST-Tag activity 3 uM histone H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD2 (344-455), GST-tag protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
BRD2, Recombinant Protein (Cat# AAA60159)
Full Name
Recombinant BRD2 (344-455) protein, GST-Tag
Pricing
Application Data
(HTRF assay for KDM7A activity. 1 uM H3K9me2 peptide was incubated with KDM7A protein in reaction buffer with or without 0.02% Triton X-100, respectively, for 1 hour at room temperature. KDM7A enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K9me0) was measured using H3K9me0-specific antibody. The results show that 0.02% Triton X-100 slightly inhibits the activity of KDM7A.)
KDM7A, Recombinant Protein (Cat# AAA59759)
Full Name
Recombinant KDM7A protein
Gene Names
KDM7A; JHDM1D
Purity
The recombinant protein is >70% pure by SDS-PAGE.
Pricing
WB (Western Blot)
(Western Blot analysis for Mononucleosomes H3K4me3 (EPL) - biotinylated. Unmodified mononucleosomes (Lane 1) and Mononucleosomes H3K4me3 (Lane 2) were detected with anti-H3K4me3 antibody and anti-H4 antibody, respectively. H4 was detected as loading control. Only Mononucleosomes H3K4me3 can be detected by anti- H3K4me3 antibody. Nucleosomes = Mononucleosomes)
Mononucleosomes H3K4me3, Recombinant Protein (Cat# AAA59763)
Full Name
Recombinant Mononucleosomes H3K4me3 (EPL)-biotinylated
Gene Names
HIST1H2AC; H2A/l; H2AFL; dJ221C16.4
Pricing
WB (Western Blot)
(Western blot for recombinant CREBBP (1075-1873) protein activity 0.5 ?g Histone H3 was incubated with 0 ?g (-), 0.5 ?g (+) CREBBP (1075-1873) in 20 ?l reaction system for 2 hours at room temperature. Half of each reaction was run on a 13% SDS-PAGE gel, and products were detected by Western blot. H3ac (pan-acetyl) antibody was used to recognize acetylated histone H3.)
CREBBP, Recombinant Protein (Cat# AAA59768)
Full Name
Recombinant CREBBP (1075-1873) protein
Gene Names
CREBBP; CBP; RSTS; KAT3A
Pricing
Application Data
(Recombinant PTPN2 protein activity assay. 1 mM pNPP was incubated with different concentrations of PTPN2 protein ina 100 ul reaction with buffer including 50 mM HEPES pH 7.5, 2 mM EDTA, 3 mM DTT, 100 mM NaCl at RT. The absorbance at 405 nm was continuously monitored for 30 minutes.)
PTPN2, Recombinant Protein (Cat# AAA59770)
Full Name
Recombinant PTPN2 protein
Gene Names
PTPN2; PTN2; PTPT; TCPTP; TC-PTP; TCELLPTP
Pricing
Application Data
(ESI-TOF Mass Spec analysis for Recombinant Histone H3K9me2 (MLA). Expected mass: 15271 Da Observed mass: 15271 Da)
Histone H3K9me2, Recombinant Protein (Cat# AAA59773)
Full Name
Recombinant Histone H3K9me2 (MLA)
Gene Names
HIST1H3A; H3/A; H3FA
Pricing
Application Data
(ESI-TOF Mass Spec analysis for Recombinant Histone H3K4me3 (MLA). Expected mass: 15285 Da Observed mass: 15285 Da)
Histone H3K4me3, Recombinant Protein (Cat# AAA59777)
Full Name
Recombinant Histone H3K4me3 (MLA)
Gene Names
HIST1H3A; H3/A; H3FA
Pricing
Application Data
(Recombinant IDH1 protein activity assay. 200 uM DL-isocitric acid trisodium and 200 uM NADP+ were incubated with IDH1 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.)
Application Data
(ESI-TOF Mass Spec analysis for Recombinant Histone H3K36me1 (MLA). Expected mass: 15257 Da Observed mass: 15257 Da)
Histone H3K36me1, Recombinant Protein (Cat# AAA59791)
Full Name
Recombinant Histone H3K36me1 (MLA)
Gene Names
HIST1H3A; H3/A; H3FA
Pricing
Application Data
(MST1 activity assay. Recombinant MST1 activity measured using a fluorescent kinase assay.)
MST1, Recombinant Protein (Cat# AAA59637)
Full Name
Recombinant MST1 protein
Gene Names
STK4; KRS2; MST1; YSK3; DKFZp686A2068
Pricing
Application Data
(Recombinant BRD7 (129-236) HTRF activity assay. 3 uM histone peptide H4K5/8/12/16 (4Ac) was incubated with BRD7 (129-236) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products)
BRD7, Recombinant Protein (Cat# AAA59649)
Full Name
Recombinant BRD7 (129-236) protein
Purity
The recombinant protein is >85% pure by SDS-PAGE.
Pricing
Application Data
(Recombinant EHMT2 (G9A)-SET (913-1193) activity assay. 3.3 uM H3 (1-21) peptide was incubated with EHMT2 (G9A)-SET (913-1193 protein in reaction buffer including 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 3 hours at room temperature. H3K9me2 antibody is used to detect reaction product. )
EHMT2, Recombinant Protein (Cat# AAA59675)
Full Name
Recombinant EHMT2 (G9A)-SET (913-1193) protein
Gene Names
EHMT2; G9A; BAT8; GAT8; NG36; KMT1C; C6orf30
Pricing
Application Data
(Recombinant SETD8 protein activity assay. Recombinant SETD8 activity titration was assessed using a Radiometry assay. Titration curves were generated to show signal response in the presence of histone peptide substrate at increasing SETD8 concentrations. This data was generated and kindly provided courtesy of ChemPartner.)
SETD8, Recombinant Protein (Cat# AAA59677)
Full Name
Recombinant SETD8 protein
Gene Names
SETD8; SET8; KMT5A; SET07; PR-Set7
Purity
The recombinant protein is >85% pure by SDS-PAGE.
Pricing
Application Data
(HTRF assay for Recombinant PHF8 protein activity. 1 uM H3K4me3K9me1 peptide was incubated with PHF8 / KDM7B protein in reaction buffer for 1 hour at room temperature. Anti-H3K9me0 antibody was used to detect reaction product.)
PHF8, Recombinant Protein (Cat# AAA59682)
Full Name
Recombinant PHF8 protein
Gene Names
PHF8; KDM7B; JHDM1F; MRXSSD; ZNF422
Pricing
Application Data
(HTRF assay for ASH1L (2407-2579) activity 3 uM histone H4K5/8/12/16(ac4) peptide was incubated with different concentrations of ASH1L (2407-2579) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
ASH1L, Recombinant Protein (Cat# AAA59687)
Full Name
Recombinant ASH1L (2407-2579) protein
Gene Names
ASH1L; ASH1; KMT2H; ASH1L1
Pricing
Application Data
(Recombinant BRD4 (333-460) HTRF activity assay 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRD4 (333-460) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products)
BRD4, Recombinant Protein (Cat# AAA59688)
Full Name
Recombinant BRD4 (333-460) protein
Gene Names
BRD4; CAP; MCAP; HUNK1; HUNKI
Purity
The recombinant protein is >87% pure by SDS-PAGE.
Pricing
Application Data
(HTRF assay for Recombinant SETDB1 protein activity. 3 uM histone peptide H3 (1-21) was incubated with SETDB1 in reaction buffer including 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 3 hours at room temperature. H3K9me2 antibody was used to detect reaction product.)
Application Data
(JHDM1D-l activity assay. Recombinant JHDM1D-l activity measured using a fluorescence-based demethylation assay.)
JHDM1D-l, Recombinant Protein (Cat# AAA59698)
Full Name
Recombinant JHDM1D-l protein
Gene Names
KDM7A; JHDM1D
Pricing
WB (Western Blot)
(Histone methyltransferase activity assay comparing recombinant nucleosomes and histone octamers as substrates. 2 ug Recombinant mononucleosomes (H3.1) were incubated with 0 ug (-), 0.05 ug (+), 0.1 ug (++), 0.2 ug (+++) NSD2-SET protein, respectively, in 30 ul reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 h at room temperature. 6 ul reaction products were loaded and run on a 12.5% SDS-PAGE gel. Western Blot was used to detect the generation of reaction products (H3K36me2). The Western blot result shows that recombinant mononucleosomes (H3.1) are better substrate than histone octamer for NSD2-SET assay.)
Mononucleosomes, Recombinant Protein (Cat# AAA59701)
Full Name
Recombinant Mononucleosomes (H3.1)-biotinylated
Purity
The recombinant protein is >95% pure by SDS-PAGE.
Pricing
Application Data
(Recombinant EZH1 complex HTRF activity assay 3.3 ?M H3K27me0 (16-37aa) peptide was incubated with EZH1 complex in reaction buffer for 3 hour at room temperature. EZH1 complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K27me1) was measured using H3K27me1-specific antibody.)
EZH1, Recombinant Protein (Cat# AAA59721)
Full Name
Recombinant EZH1 complex
Gene Names
EZH1; KMT6B
Purity
The recombinant protein is >95% pure by SDS-PAGE.
Pricing
Application Data
(HTRF assay for SIRT2 (50-356) activity.3 uM histone peptide H3K9ac (1-21) was incubated with SIRT2 (50-356) in reaction buffer for 30 min at 37 degree C. Anti-H3K9me0 antibody was used to detect reaction products.)
SIRT2, Recombinant Protein (Cat# AAA59736)
Full Name
Recombinant SIRT2 (50-356) protein
Purity
Recombinant SIRT2 (50-356) protein that includes amino acids 50-356 of human SIRT2 protein (accession number NM_012237.3) was expressed in E Coli and contains an N-terminal GST-Tag with a molecular weight of 66.5 kDa.The purity ofThe protein is >= 85% by
Pricing
Application Data
(HTRF assay for SIRT3 (102-399) activity.3 uM histone peptide H3K9ac (1-21) was incubated with SIRT3 (102-399) in reaction buffer for 30 min at 37 degree C. Anti-H3K9me0 antibody was used to detect reaction products.)
SIRT3, Recombinant Protein (Cat# AAA59737)
Full Name
Recombinant SIRT3 (102-399) protein
Purity
Recombinant SIRT3 (102-399) protein that includes amino acids 102-399 of human SIRT3 protein (accession number NM_012239.5) was expressed in E Coli and contains an N-terminal GST-Tag with a molecular weight of 59.7 kDa.The purity ofThe protein is > 60% by
Pricing
SDS-PAGE
(SDS-PAGE: purified spike SARS-CoV-2 S1 protein of the UK variant)
COVID 19 Spike S1 Protein (UK Variant B.1.1.7) Coronavirus, Recombinant Protein (Cat# AAA62225)
Full Name
Spike S1 Protein (SARS-CoV-2 UK Variant B.1.1.7)
Applications
Western Blot
Purity
>= 95% (SDS-PAGE)
Pricing
What Are Recombinant Proteins?
A recombinant protein is created by inserting the gene of interest into a host system like E. coli, mammalian cells, or insect cells. This protein expression method results in the large-scale production of protein in a controlled environment with highly precise structures and functionality. Proteins produced via the “Recombinant” methodology have proven themselves crucial in various biological research areas, including immunology, neuroscience, cancer research, drug delivery and development, and more.
Core Recombinant Protein Product Lines:
- Human: Produced from human genes (e.g., growth factors, cytokines).
- Animal: Derived from mouse, rat, or other animals (e.g., mouse antibodies, enzymes).
- Bacterial: Produced in bacteria like E. coli (e.g., enzymes, bacterial toxins).
- Yeast: Produced in yeast cells (e.g., surface proteins, metabolic enzymes).
- Insect:Expressed in insect cells (e.g., viral proteins, glycoproteins).
- Viral: Derived from viral genomes (e.g., spike proteins for vaccine development).
How Does It Work?
- Gene Identification and Cloning: Scientists first find the exact gene that codes for the protein they are interested in producing, and then create a copy of said gene.
- Recombinant DNA Creation: The copied gene is then inserted into a vector (a vehicle-like plasmid) that can be transported safely into your intended expression host cell/system.
- Host Cell Transformation: The vector (with the foreign gene inside) is shuttled into a host cell. This host can be a bacterium (like E. coli), yeast, insect cells, or mammalian cells, depending on the type of protein or protein characteristics needed.
- Protein Expression:The host cell “reads” the copied gene that was shuttled into it, and begins to produce the gene’s coded-for protein using its own cellular machinery. The host can continue to multiply, and with each new cell, more protein is produced.
- Protein Purification:After enough protein has been made, scientists collect the host cell and separate out the target protein. The protein is then carefully purified so it is free of contaminants, ideally active, and also safe for use.
The result is a purified recombinant protein — a lab-made protein that is biologically identical (in most respects) to the natural/native version.
We take this process a step further, offering recombinant proteins that are produced with precise control over sequence modifications, expression levels, and large-scale production. This ensures high-quality, consistent results that will be able to support your research needs and empower your scientific discoveries.
Some of the expression hosts/systems used in recombinant protein generation by AAA Biotech include:
- Escherichia coli (E. coli)
- Human (Homo sapiens)
- Chinese Hamster
- Yeast
- Insect cells (e.g., Baculovirus expression system)
Advantages Of Our Recombinant Proteins:
Recombinant Protein Offerings
| S.No | Protein Expression Systems | Quality and Bioactivity | Formats and Flexibility |
|---|---|---|---|
| 01. | E. coli, HEK293, CHO, yeast, and insect cells | Validated high bioactivity and binding activity | Ready-to-use formats (lyophilized or liquid formulations available) |
| 02. | 675+ recombinant protein products | High protein purity (≥95%) | Available in flexible pack sizes |
| 03. | Custom protein production (His-tag, GST, FLAG, and Fc fusion) | Lot-to-lot consistency through QC protocols | Wide range of targets (Cytokines, growth factors, enzymes, receptors, signaling proteins, etc.) |
Applications Of Our Recombinant Proteins
- Studying protein interactions (protein–protein or protein–DNA).
- Testing biological pathways with functional/activity assays.
- Creating standard curves in ELISA and other tests.
- Making antigens/immunogens for antibody production.
- Drug discovery and screening.
- Finding and confirming biomarkers.
- Researching cell signaling and immunity.
- Developing vaccines.
Read more about the applications of the recombinant proteins here!
Why Buy Recombinant Proteins from AAA Biotech?
1. High Purity & Verified Quality:
Most of our proteins are ≥ 95% pure. We also test the biological activity of our products where it's relevant (it will be indicated on the product page).
2. Multiple Expression Systems:
We offer proteins made in multiple host systems, and this enables researchers to pick the system that gives them the best folding, post-translational modifications, or functionality for their experiment requirements.
3. Custom & Flexible Options:
Need a special tag (His, GST, FLAG, Fc fusion)? Or a custom variant? We can provide it. Our proteins come in formats that are lab-friendly: lyophilized (dry) or “ready-to-use” liquid.
4. Rigorous Quality Control & Documentation:
Every batch is backed by strong QC. This means you can rely on product consistency — two different batches will perform similarly.
5. Wide Variety & Global Availability:
We carry a large and growing catalog of recombinant proteins to cover almost all needs in research (immunology, oncology, vaccine work, etc.)
6. Researcher-Focused Support:
Technical support, clear documentation, and user-friendly product pages help you select the right protein for your work. Plus, our user-friendly packaging and handling, and global shipping are all designed to reduce delays, damage, and hassles.
Order Recombinant Proteins Today!
We are committed to supporting the research community with recombinant proteins that display exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
We have a track record of delivering proteins that work — large numbers of researchers rely on them in many published studies. Browse our catalog to find the perfect protein for your research applications now!
FAQ
1. What is involved in the purification of recombinant proteins?
The purification process of these recombinant proteins is performed to isolate the specific protein produced by the host cell from unwanted substances. The process generally includes techniques such as “affinity chromatography” to separate the protein from other proteins and impurities. AAA Biotech generally offers proteins ≥ 95% pure, depending on the protein type.
2. What are some examples of popular AAA Biotech recombinant proteins?
Some examples of recombinant proteins include:
Retinoblastoma Protein (Cat # AAA10852) – reportedly used in vaccine research and antibody development. Hepatitis B Surface Antigen (HBsAg) (Cat# AAA13446) – reportedly used in diagnostic tests and vaccine development for Hepatitis B. High-Mobility Group Box 1 (HMGB1) (Cat# AAA10851) – reportedly used in inflammation and immune response research.
3. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product (only upon request for some products).
4. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
5. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.