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product-image-AAA263141_SC15.jpg Standard Curve (Sample)

Mouse MRP8 ELISA Kit | S100A8 elisa kit

Mouse MRP8 ELISA Kit

Gene Names
S100a8; p8; B8Ag; CFAg; Caga; MRP8; CP-10; 60B8Ag; AI323541
Reactivity
Mouse
Synonyms
MRP8; N/A; Mouse MRP8 ELISA Kit; CAGA; CFAG; Protein S100-A8; Calgranulin-A; Calprotectin L1L subunit; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; S100 calcium-binding protein A8; Urinary stone protein band A; S100A8 elisa kit
Ordering
Reactivity
Mouse
Specificity
The Mouse MRP8 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse MRP8 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
Sequence Length
89
Samples
Cell Culture Supernates, Serum, Plasma
Sensitivity
<10 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Standard Curve (Sample)

product-image-AAA263141_SC15.jpg Standard Curve (Sample)
Related Product Information for S100A8 elisa kit
Principle of the Assay: The Mouse MRP8 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse MRP8 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse MRP8 coated on a 96-well plate. Standards and samples are pipetted into the wells and MRP8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse MRP8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MRP8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: S100 calcium-binding protein A8 (S100A8), also known as calgranulin A, is a protein that in humans is encoded by the S100A8 gene. It is mapped to chromosome 1q21.3 based on an alignment of the S100A8 sequence with the genomic sequence. The proteins S100A8 and S100A9 form a heterodimer called calprotectin which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. This gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. And S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,295 Da
NCBI Official Full Name
protein S100-A8
NCBI Official Synonym Full Names
S100 calcium binding protein A8 (calgranulin A)
NCBI Official Symbol
S100a8
NCBI Official Synonym Symbols
p8; B8Ag; CFAg; Caga; MRP8; CP-10; 60B8Ag; AI323541
NCBI Protein Information
protein S100-A8
UniProt Protein Name
Protein S100-A8
UniProt Gene Name
S100a8
UniProt Synonym Gene Names
Caga; Mrp8; MRP-8; p8
UniProt Entry Name
S10A8_MOUSE

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Product Notes

The Mouse S100A8 s100a8 (Catalog #AAA263141) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA263141 ELISA Kit recognizes Mouse S100A8. It is sometimes possible for the material contained within the vial of "MRP8, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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