Application Data
(Figure-3: Comparative binding of Spike S1 variants to sACE2: Wells of a 96-well microtiter plate were coated with 100 ng in duplicates each of S1-WT, S1-N439K, S1-D614G, S1-South Africa, and S1-Southern California. Binding to sACE2 was determined by adding different concentrations of biotinylated-sACE2.)
SARS-CoV-2 (2019-nCoV) Spike S1 (D614G) Recombinant Protein | SARS-CoV-2 recombinant protein
SARS-CoV-2 (2019-nCoV) Spike S1 (D614G) His Tag Protein
Applications
Western Blot, ELISA
Purity
>95% by SDS-PAGE.
Synonyms
SARS-CoV-2 (2019-nCoV) Spike S1 (D614G); N/A; SARS-CoV-2 (2019-nCoV) Spike S1 (D614G) His Tag Protein; SARS-CoV-2 recombinant protein
Host
CHO-K1
Purity/Purification
>95% by SDS-PAGE.
Form/Format
PBS and 10% Glycerol.
Applicable Applications for SARS-CoV-2 recombinant protein
WB (Western Blot), ELISA
Protein Construction
The target protein is expressed with sequence (AA 14-683) of SARS-CoV-2/COVID-19 Spike S1 sequence with mutation at 614aa (D614G) fused with a 10xHis tag in C-Terminal.
Preparation and Storage
SARS-CoV-2 Spike S1-His Protein is shipped on ice packs. Upon arrival, Store at -20 degree C. Do not freeze-thaw multiple times.
Application Data
(Figure-3: Comparative binding of Spike S1 variants to sACE2: Wells of a 96-well microtiter plate were coated with 100 ng in duplicates each of S1-WT, S1-N439K, S1-D614G, S1-South Africa, and S1-Southern California. Binding to sACE2 was determined by adding different concentrations of biotinylated-sACE2.)
Application Data
(Figure-3: Comparative binding of Spike S1 variants to sACE2: Wells of a 96-well microtiter plate were coated with 100 ng in duplicates each of S1-WT, S1-N439K, S1-D614G, S1-South Africa, and S1-Southern California. Binding to sACE2 was determined by adding different concentrations of biotinylated-sACE2.)
WB (Western Blot)
(Figure-2: Western blot analysis of recombinant SARS-CoV-2 Spike S1 D614G mutant protein. Anti-Spike S1 antibody was used in western blot.)
WB (Western Blot)
(Figure-2: Western blot analysis of recombinant SARS-CoV-2 Spike S1 D614G mutant protein. Anti-Spike S1 antibody was used in western blot.)
Application Data
(Figure-1: Recombinant SARS-CoV-2 Spike S1 D614G mutant protein was run on a 4-20% SDS-PAGE gel followed by Coomassie blue staining.)
Application Data
(Figure-1: Recombinant SARS-CoV-2 Spike S1 D614G mutant protein was run on a 4-20% SDS-PAGE gel followed by Coomassie blue staining.)
Related Product Information for SARS-CoV-2 recombinant protein
The spike protein (S1) of coronavirus (CoV2) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Product Categories/Family for SARS-CoV-2 recombinant protein
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Product Notes
The SARS-CoV-2 (Catalog #AAA78596) is a Recombinant Protein produced from CHO-K1 and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SARS-CoV-2 (2019-nCoV) Spike S1 (D614G) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SARS-CoV-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SARS-CoV-2 (2019-nCoV) Spike S1 (D614G), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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