Search results for "Recombinant Proteins"

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Western Blot (WB) (The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TREM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : 293T cell lysateLane 2 : TREM2 19-161aa Transfected 293T cell lysate)

TREM2, Monoclonal Antibody (Cat# AAA332953)

Full Name

Human TREM2 antibody

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Flow Cytometry

Purity

By protein-A affinity chromatography

Pricing

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Immunohistochemistry (IHC)-Formalin (Formalin-fixed, paraffin-embedded human cardiac muscle stained with Dystrophin Mouse Monoclonal Antibody (DMD/3677).)

Dystrophin (DMD), Monoclonal Antibody (Cat# AAA333002)

Full Name

Dystrophin (DMD) (Marker of Duchenne and Becker Muscular Dystrophy)

Gene Names

DMD; BMD; CMD3B; MRX85; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272

Reactivity

Human

Applications

EIA, IHC

Purity

Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug HIF1AN antibody. Western blot was performed from the immunoprecipitate using HIF1AN antibody at a dilition of 1:1000.)

HIF1AN, Polyclonal Antibody (Cat# AAA28174)

Full Name

HIF1AN Polyclonal Antibody

Gene Names

HIF1AN; FIH1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation

Purity

Affinity Purification

Pricing

IF (Immunofluorescence) (Figure 9. IF analysis of ATP5D/ATP5F1D using anti-ATP5D/ATP5F1D antibody (AAA20015).ATP5D/ATP5F1D was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ATP5D/ATP5F1D Antibody (AAA20015) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ATP5D/ATP5F1D Antibody (AAA20015) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ATP5D/ATP5F1D Antibody (AAA20015) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ATP5D/ATP5F1D Antibody (AAA20015) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ATP5D/ATP5F1D Antibody (AAA20015) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ATP5D/ATP5F1D Antibody (AAA20015) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ATP5D/ATP5F1D Antibody (AAA20015) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human Raji whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: rat kidney tissue lysates,Lane 7: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5D/ATP5F1D antigen affinity purified polyclonal antibody (#AAA20015) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATP5D/ATP5F1D at approximately 17 kDa. The expected band size for ATP5D/ATP5F1D is at 17 kDa.)

ATP5F1D, Polyclonal Antibody (Cat# AAA20015)

Full Name

Anti-ATP5F1D Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Purity

Immunogen affinity purified.

Pricing

Retinoblastoma, Recombinant Protein (Cat# AAA10852)

Full Name

Recombinant Human Retinoblastoma Associated Protein

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

Purity

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using Bcl-2 Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Bcl-2, Polyclonal Antibody (Cat# AAA28424)

Full Name

Bcl-2 Mouse mAb

Gene Names

VEGFA; VPF; VEGF; MVCD1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

IHC (Immunohistochemistry) (Figure 7. IHC analysis of GOLGA7 using anti-GOLGA7 antibody (AAA19952).GOLGA7 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLGA7 Antibody (AAA19952) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLGA7 Antibody (AAA19952) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLGA7 Antibody (AAA19952) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLGA7 Antibody (AAA19952) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLGA7 Antibody (AAA19952) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLGA7 Antibody (AAA19952) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLGA7 Antibody (AAA19952) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

GOLGA7, Polyclonal Antibody (Cat# AAA19952)

Full Name

Anti-GOLGA7 Antibody

Gene Names

GOLGA7; GCP16; GOLGA7A; HSPC041; GOLGA3AP1

Reactivity

Human

Applications

Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-LDLR antibody (AAA126965).Overlay histogram showing MCF-7 cells stained with AAA126965 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LDLR Antibody (AAA126965, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

LDLR, Polyclonal Antibody (Cat# AAA126965)

Full Name

Anti-LDLR Antibody Picoband

Gene Names

LDLR; FH; FHC; LDLCQ2

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Figure 1. Western blot analysis of FOXK1 using anti-FOXK1 antibody.)

FOXK1/MNF, Polyclonal Antibody (Cat# AAA19209)

Full Name

Anti-FOXK1/MNF Antibody

Gene Names

FOXK1; FOXK1L

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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Application Data (Detection limit for recombinant GST tagged PTBP1 is ~0.1ng/ml as a capture antibody.)

PTBP1, Monoclonal Antibody (Cat# AAA25809)

Full Name

PTBP1 (Polypyrimidine Tract-binding Protein 1, PTB, Heterogeneous Nuclear Ribonucleoprotein I, hnRNP I, 57kD RNA-binding Protein PPTB-1, MGC10830, MGC8461) (PE)

Gene Names

PTBP1; PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using BAG1 antibody. Blue: DAPI for nuclear staining.)

BAG1, Polyclonal Antibody (Cat# AAA10702)

Full Name

BAG1 Polyclonal Antibody

Gene Names

BAG1; HAP; BAG-1; RAP46

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug TP53 antibody. Western blot was performed from the immunoprecipitate using TP53 antibody at a dilition of 1:500.)

TP53, Polyclonal Antibody (Cat# AAA28134)

Full Name

TP53 Polyclonal Antibody

Gene Names

Trp53; bbl; bfy; bhy; p44; p53; Tp53

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U87 cells using anti-Scavenging Receptor SRB2/SCARB2 antibody (AAA19535).Overlay histogram showing U87 cells stained with AAA19535 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Scavenging Receptor SRB2/SCARB2 Antibody (AAA19535, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Scavenging Receptor SRB2/SCARB2, Polyclonal Antibody (Cat# AAA19535)

Full Name

Anti-Scavenging Receptor SRB2/SCARB2 Antibody Picoband

Gene Names

SCARB2; AMRF; EPM4; LGP85; CD36L2; HLGP85; LIMP-2; LIMPII; SR-BII

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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hnRNP L/HNRNPL, Polyclonal Antibody (Cat# AAA19499)

Full Name

Anti-hnRNP L/HNRNPL Antibody Picoband

Gene Names

HNRNPL; HNRPL; hnRNP-L; P/OKcl.14

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-MCCC2 antibody (AAA19873).Overlay histogram showing MCF-7 cells stained with AAA19873 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCCC2 Antibody (AAA19873, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of MCCC2 using anti-MCCC2 antibody (AAA19873).MCCC2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MCCC2 Antibody (AAA19873) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MCCC2 Antibody (AAA19873) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MCCC2 Antibody (AAA19873) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MCCC2 Antibody (AAA19873) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MCCC2 Antibody (AAA19873) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MCCC2 Antibody (AAA19873) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MCCC2 Antibody (AAA19873) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human RT4 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: rat kidney tissue lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCCC2 antigen affinity purified polyclonal antibody (#AAA19873) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MCCC2 at approximately 61 kDa. The expected band size for MCCC2 is at 61 kDa.)

MCCC2, Polyclonal Antibody (Cat# AAA19873)

Full Name

Anti-MCCC2 Antibody Picoband

Gene Names

MCCC2; MCCB

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Figure 6. IF analysis of CD72 using anti- CD72 antibody (AAA19329).CD72 was detected in immunocytochemical section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- CD72 Antibody (AAA19329) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

CD72, Polyclonal Antibody (Cat# AAA19329)

Full Name

Anti-CD72 Antibody

Gene Names

Cd72; CD72c; Ly-19; Ly-32; Lyb-2; Ly-m19

Reactivity

Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using VAPB antibody. Blue: DAPI for nuclear staining.)

VAPB, Polyclonal Antibody (Cat# AAA28162)

Full Name

VAPB Polyclonal Antibody

Gene Names

VAPB; ALS8; VAP-B; VAMP-B

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

High-Mobility Group Box 1, Recombinant Protein (Cat# AAA10851)

Full Name

Recombinant Human High-Mobility Group Box 1

Gene Names

HMGB1; HMG1; HMG3; SBP-1

Purity

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Pricing

Centromere Protein-B, Recombinant Protein (Cat# AAA10838)

Full Name

Recombinant Human Centromere Protein-B

Applications

Western Blot

Purity

Greater than 95% as determined by SDS-PAGE.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat brain using SCAF4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

SCAF4, Polyclonal Antibody (Cat# AAA28418)

Full Name

SCAF4 Rabbit pAb

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

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Application Data (Detection limit for recombinant GST tagged S100A6 is ~0.03ng/ml as a capture antibody.)

S100A6, Monoclonal Antibody (Cat# AAA25829)

Full Name

S100A6 (Protein S100-A6, S100 Calcium-binding Protein A6, Calcyclin, Prolactin Receptor-associated Protein, PRA, Growth Factor-inducible Protein 2A9, MLN 4, CACY) (PE)

Gene Names

S100A6; 2A9; PRA; 5B10; CABP; CACY

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

Cullin-1, Recombinant Protein (Cat# AAA10882)

Full Name

Recombinant Human Cullin-1

Purity

Greater than 95% as determined by SDS-PAGE.

Pricing

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Procollagen III N-Terminal Propeptide, Recombinant Protein (Cat# AAA21046)

Full Name

Recombinant Procollagen III N-Terminal Propeptide (PIIINP)

Reactivity

Homo sapiens (Human)

Applications

Western Blot

Purity

> 90%

Pricing

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Inhibin Beta E (INHbE), Recombinant Protein (Cat# AAA20279)

Full Name

Recombinant Inhibin Beta E (INHbE)

Reactivity

Homo sapiens (Human)

Applications

Western Blot

Purity

> 90%

Pricing

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Thrombin/Antithrombin Complex (TAT), Recombinant Protein (Cat# AAA21174)

Full Name

Recombinant Thrombin/Antithrombin Complex (TAT)

Reactivity

Homo sapiens (Human)

Applications

Western Blot

Purity

> 80%

Pricing

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Plasmin/Antiplasmin Complex (PAP), Recombinant Protein (Cat# AAA21146)

Full Name

Recombinant Plasmin/Antiplasmin Complex (PAP)

Reactivity

Homo sapiens (Human)

Applications

Western Blot

Purity

> 95%

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of 293T cells using anti-ABR antibody (AAA19758).Overlay histogram showing 293T cells stained with AAA19758 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABR Antibody (AAA19758, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 5. IF analysis of ABR using anti-ABR antibody (AAA19758).ABR was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ABR Antibody (AAA19758) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ABR Antibody (AAA19758) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ABR Antibody (AAA19758) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABR antigen affinity purified polyclonal antibody (#AAA19758) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ABR at approximately 100 kDa. The expected band size for ABR is at 98 kDa.)

ABR, Polyclonal Antibody (Cat# AAA19758)

Full Name

Anti-ABR Antibody Picoband

Gene Names

ABR; MDB

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-NUBPL antibody (AAA19948).Overlay histogram showing MCF-7 cells stained with AAA19948 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUBPL Antibody (AAA19948, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of NUBPL using anti-NUBPL antibody (AAA19948).NUBPL was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUBPL Antibody (AAA19948) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUBPL Antibody (AAA19948) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUBPL Antibody (AAA19948) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human T-47D whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human A431 whole cell lysates,Lane 5: human Colo320 whole cell lysates,Lane 6: human MCF-7 whole cell lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUBPL antigen affinity purified polyclonal antibody (#AAA19948) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NUBPL at approximately 34 kDa. The expected band size for NUBPL is at 34 kDa.)

NUBPL, Polyclonal Antibody (Cat# AAA19948)

Full Name

Anti-NUBPL Antibody Picoband

Gene Names

NUBPL; IND1; huInd1; C14orf127

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

AIG2-Like Domain 1, Recombinant Protein (Cat# AAA10884)

Full Name

Recombinant Human AIG2-Like Domain 1

Gene Names

GGACT; A2LD1

Purity

Greater than 95.0% as determined by SDS-PAGE.

Pricing

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IF (Immunofluorescence) (Figure 6. IF analysis of MIRO1/RHOT1 using anti-MIRO1/RHOT1 antibody (AAA19560).MIRO1/RHOT1 was detected in an immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-MIRO1/RHOT1 Antibody (AAA19560) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

MIRO1/RHOT1, Polyclonal Antibody (Cat# AAA19560)

Full Name

Anti-MIRO1/RHOT1 Antibody Picoband

Gene Names

RHOT1; ARHT1; MIRO1; MIRO-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Purity

Immunogen affinity purified.

Pricing

Der P1, Recombinant Protein (Cat# AAA10807)

Full Name

Recombinant Der P1 Protein

Purity

Protein is >95% pure as determined by 10% SDS-PAGE (coomassie staining)
Purified by proprietary chromatographic technique.

Pricing

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Prostate Stem Cell Antigen (PSCA), Recombinant Protein (Cat# AAA20218)

Full Name

Recombinant Prostate Stem Cell Antigen (PSCA)

Reactivity

Homo sapiens (Human)

Applications

Western Blot, Immunoprecipitation

Purity

>98%

Pricing

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Alpha-synuclein, Monoclonal Antibody (Cat# AAA28601)

Full Name

Alpha-synuclein Mouse mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

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Microtubule Associated Protein 2 (MAP2), Recombinant Protein (Cat# AAA20281)

Full Name

Recombinant Microtubule Associated Protein 2 (MAP2)

Gene Names

MAP2; MAP2A; MAP2B; MAP2C

Reactivity

Homo sapiens (Human)

Applications

Western Blot

Purity

> 97%

Pricing

Pru P 1, Recombinant Protein (Cat# AAA24012)

Full Name

Pru P 1 protein

Applications

Western Blot

Purity

> 95% by SDS-PAGE

Pricing

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WB (Western Blot) (Figure 6 WB Validation of Delta Variant Spike RBD Protein with SARS-CoV-2 Spike RBD Antibodies Loading: 20 ng of SARS-CoV-2 spike RBD proteins, including WT and Delta variant (B.1.671.2).Detection Antibodies: SARS-CoV-2 Spike RBD antibody, 1 μg/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.)

COVID 19 Delta Variant Spike RBD (His-Avi Tag) Coronavirus, Recombinant Protein (Cat# AAA11039)

Full Name

SARS-CoV-2 (COVID-19) Delta Variant Spike RBD (His-Avi Tag) Recombinant Protein

Applications

Western Blot

Purity

> 95% as determined by Bis-Tris PAGE

Pricing

HCV E2 protein, Recombinant Protein (Cat# AAA13770)

Full Name

HCV E2 protein (aa 383-663), Subtype 1a

Applications

Western Blot

Purity

>= 95% purity

Pricing

Mammaglobin-A, Recombinant Protein (Cat# AAA10805)

Full Name

Recombinant Human Mammaglobin-A, HEK

Gene Names

SCGB2A2; MGB1; UGB2

Purity

Greater than 95.0% as determined by SDS-PAGE

Pricing

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Carbohydrate Antigen 125 (CA125), Recombinant Protein (Cat# AAA20282)

Full Name

Recombinant Carbohydrate Antigen 125 (CA125)

Gene Names

MUC16; CA125

Reactivity

Homo sapiens (Human)

Applications

Western Blot

Purity

> 95%

Pricing

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ELISA (Figure 6 ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Spike S1 Antibodies Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 ˚C overnight.Detection Antibodies: SARS-CoV-2 Spike S1 antibody, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.Antibody 9083 does not distinguish between WT and Delta.)

COVID 19 Delta Variant Spike S1 (His-Avi Tag) Coronavirus, Recombinant Protein (Cat# AAA11040)

Full Name

SARS-CoV-2 (COVID-19) Delta Variant Spike S1 (His-Avi Tag) Recombinant Protein

Applications

Western Blot

Purity

> 90% as determined by Bis-Tris PAGE

Pricing

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Fibroblast Growth Factor 10 (FGF10), Recombinant Protein (Cat# AAA20261)

Full Name

Recombinant Fibroblast Growth Factor 10 (FGF10)

Gene Names

Fgf10; AEY17; Fgf-10; BB213776; Gsfaey17

Reactivity

Mus musculus (Mouse)

Applications

Western Blot

Purity

> 97%

Pricing

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IF (Immunofluorescence) (Figure 8. IF analysis of TRK fused gene/TFG using anti-TRK fused gene/TFG antibody (AAA19472).TRK fused gene/TFG was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-TRK fused gene/TFG Antibody (AAA19472) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

TRK fused gene/TFG, Polyclonal Antibody (Cat# AAA19472)

Full Name

Anti-TRK fused gene/TFG Antibody Picoband

Gene Names

TFG; TF6; HMSNP; SPG57; TRKT3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil using von Willebrand factor (VWF) Rabbit mAb (AAA28546) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)