Search results for "Recombinant Proteins"

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Troponin T Type 1, Slow Skeletal (TNNT1), Recombinant Protein (Cat# AAA20264)

Full Name

Recombinant Troponin T Type 1, Slow Skeletal (TNNT1)

Gene Names

TNNT1; ANM; TNT; NEM5; STNT; TNTS

Reactivity

Homo sapiens (Human)

Applications

Western Blot

Purity

> 90%

Pricing

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Collagen Type I Alpha 1 (COL1a1), Recombinant Protein (Cat# AAA20234)

Full Name

Recombinant Collagen Type I Alpha 1 (COL1a1)

Gene Names

Col1a1; Cola1; Mov13; Cola-1; Mov-13; Col1a-1

Applications

Western Blot

Purity

> 90%

Pricing

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Apolipoprotein B100 (APOB100), Recombinant Protein (Cat# AAA21115)

Full Name

Recombinant Apolipoprotein B100 (APOB100)

Gene Names

APOB; FLDB; LDLCQ4; apoB-48; apoB-100

Reactivity

Homo sapiens (Human)

Applications

Western Blot

Purity

> 90%

Pricing

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IF (Immunofluorescence) (Figure 9. IF analysis of PGP using anti-PGP antibody (AAA19740).PGP was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-PGP Antibody (AAA19740) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 6. IF analysis of PGP using anti-PGP antibody (AAA19740).PGP was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PGP Antibody (AAA19740) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PGP Antibody (AAA19740) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PGP Antibody (AAA19740) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PGP Antibody (AAA19740) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MCF-7 whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: rat lung tissue lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse lung tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGP antigen affinity purified polyclonal antibody (#AAA19740) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PGP at approximately 36 kDa. The expected band size for PGP is at 34 kDa.)

PGP, Polyclonal Antibody (Cat# AAA19740)

Full Name

Anti-PGP Antibody Picoband

Gene Names

PGP; PGPase

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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Lamin A/C, Monoclonal Antibody (Cat# AAA28306)

Full Name

[KO Validated] Lamin A/C Rabbit mAb

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse colon using Galectin 3/Galectin 3/LGALS3 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Galectin 3/LGALS3, Polyclonal Antibody (Cat# AAA28314)

Full Name

Galectin 3/LGALS3 Rabbit pAb

Gene Names

LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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Application Data (S100A8 in NIH-3T3 mouse cell line: S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10ug/mL AAA14670 for 3 hours at RT. Cells were stained (red) and counterstained with DAPI (blue).)

S100A8, Monoclonal Antibody (Cat# AAA14670)

Full Name

S100A8 (MRP-8)

Gene Names

S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; S100A8

Reactivity

Mouse

Applications

Western Blot, Immunocytochemistry

Purity

Purified by Protein G affinity chromatography from hybridoma culture supernatant.

Pricing

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Selenoprotein P1, Plasma (SEPP1), Recombinant Protein (Cat# AAA20267)

Full Name

Recombinant Selenoprotein P1, Plasma (SEPP1)

Gene Names

SEPP1; SeP; SELP

Reactivity

Homo sapiens (Human)

Applications

Western Blot

Purity

> 97%

Pricing

Leptin, ELISA Kit (Cat# AAA30734)

Full Name

Leptin (Human) OmniKine ELISA Kit

Gene Names

LEP; OB; OBS; LEPD

Reactivity

Human

Pricing

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Apolipoprotein B (APOB), Recombinant Protein (Cat# AAA20204)

Full Name

Recombinant Apolipoprotein B (APOB)

Gene Names

APOB; FLDB; LDLCQ4

Reactivity

Homo sapiens (Human)

Applications

Western Blot

Purity

> 90%

Pricing

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IHC (Immunohistchemistry) (Formalin-fixed, paraffin-embedded human Uterus stained with Vimentin Monoclonal Antibody (VM1170).)

Vimentin, Monoclonal Antibody (Cat# AAA13829)

Full Name

Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody

Gene Names

VIM; HEL113; CTRCT30

Reactivity

Human.
Does not react with Mouse and Rat

Applications

Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry

Pricing

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Application Data (Proximity Ligation Analysis of protein-protein interactions between TP53 and MDM2 HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-MDM2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

MDM2, Monoclonal Antibody (Cat# AAA24860)

Full Name

MDM2 (E3 Ubiquitin-protein Ligase Mdm2, Double Minute 2 Protein, Hdm2, Oncoprotein Mdm2, p53-binding Protein Mdm2) (Biotin)

Gene Names

MDM2; HDMX; hdm2; ACTFS

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of PC-3 cells using anti-MYCBP antibody (AAA19864).Overlay histogram showing PC-3 cells stained with AAA19864 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYCBP Antibody (AAA19864, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of MYCBP using anti-MYCBP antibody (AAA19864).MYCBP was detected in a paraffin-embedded section of human testicular germ cell tumors. tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYCBP Antibody (AAA19864) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYCBP Antibody (AAA19864) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYCBP Antibody (AAA19864) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYCBP Antibody (AAA19864) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates;Lane 3: rat lung tissue lysates;Lane 4: rat NRK whole cell lysates;Lane 5: mouse lung tissue lysates;Lane 6: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYCBP antigen affinity purified polyclonal antibody (#AAA19864) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MYCBP at approximately 12 kDa. The expected band size for MYCBP is at 12 kDa.)

MYCBP, Polyclonal Antibody (Cat# AAA19864)

Full Name

Anti-MYCBP Antibody Picoband

Gene Names

MYCBP; AMY-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western Blot analysis of AFP expression in transfected 293T cell line by AFP monoclonal antibody. Lane 1: AFP transfected lysate (69kD). Lane 2: Non-transfected lysate.)

Fetoprotein, Alpha, Monoclonal Antibody (Cat# AAA24127)

Full Name

Fetoprotein, Alpha (AFP, Alpha1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP) (AP)

Gene Names

AFP; AFPD; FETA; HPAFP

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Islet Cell Autoantigen 1 (ICA1), Recombinant Protein (Cat# AAA20268)

Full Name

Recombinant Islet Cell Autoantigen 1 (ICA1)

Gene Names

ICA1; ICA69; ICAp69

Reactivity

Homo sapiens (Human)

Applications

Western Blot

Purity

> 95%

Pricing

HA (H7N9) (A/Wuxi/2/2013), Recombinant Protein (Cat# AAA13766)

Full Name

HA (H7N9)(aa 19-535) (A/Wuxi/2/2013)

Applications

Western Blot

Purity

> = 95% (SDS-PAGE)

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human stomach using MAPK8 antibody at dilution of 1:100 (40x lens).)

MAPK8, Polyclonal Antibody (Cat# AAA10635)

Full Name

MAPK8 Polyclonal Antibody

Gene Names

MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2

Reactivity

Human, Mouse

Applications

Western Blot, Immunofluorescence

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Western Blot analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody. Lane 1: B2M transfected lysate (13.7kD). Lane 2: Non-transfected lysate.)

b2-Microglobulin, Monoclonal Antibody (Cat# AAA24866)

Full Name

b2-Microglobulin (B2M, Beta-2-microglobulin) (Biotin)

Gene Names

B2M; IMD43

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HepG2 cells using anti-RPS14 antibody (AAA19798).Overlay histogram showing HepG2 cells stained with AAA19798 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS14 Antibody (AAA19798, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IP (Immunoprecipitation) (Figure 7. Immunoprecipitating RPS14 in Hela whole cell lysate .Western blot analysis of RPS14 using anti-RPS14 antibody (AAA19798).Lane 1: Hela whole cell lysates (30ug)Lane 2: Rabbit control IgG instead of anti-RPS14 antibody in Hela whole cell lysate.Lane 3: anti-RPS14 antibody (2ug) + Hela whole cell lysate (500ug)After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RPS14 antigen affinity purified polyclonal antibody (AAA19798) at a dilution of 0.5ug/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS14 Antibody (AAA19798) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS14 Antibody (AAA19798) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS14 Antibody (AAA19798) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS14 Antibody (AAA19798) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human hepatocellular carcinoma tumor tissue (HCCT) lysates,Lane 4: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,Lane 5: rat liver tissue lysates,Lane 6: rat RH35 whole cell lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse HEPA1-6 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS14 antigen affinity purified polyclonal antibody (#AAA19798) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPS14 at approximately 16 kDa. The expected band size for RPS14 is at 16 kDa.)

RPS14, Polyclonal Antibody (Cat# AAA19798)

Full Name

Anti-RPS14 Antibody Picoband

Gene Names

RPS14; S14; EMTB

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug PPP4C antibody. Western blot was performed from the immunoprecipitate using PPP4C antibody at a dilition of 1:1000.)

PPP4C, Polyclonal Antibody (Cat# AAA10744)

Full Name

PPP4C Polyclonal Antibody

Gene Names

PPP4C; PP4; PPX; PP-X; PP4C; PPH3; PPP4

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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IHC (Immunohistchemistry) (Formalin-paraffin human Placenta stained with E-Cadherin MAb (CDH1/1525).)

E-Cadherin/CD324, Monoclonal Antibody (Cat# AAA23896)

Full Name

E-Cadherin/CD324 (Intercellular Junction Marker)

Gene Names

CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324

Reactivity

Human.
Does not react with Mouse and Rat. Others not known.

Applications

Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry

Pricing

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Application Data (Detection limit for recombinant GST tagged PHB is ~0.03ng/ml as a capture antibody.)

PHB, Monoclonal Antibody (Cat# AAA24609)

Full Name

PHB (Prohibitin) APC

Gene Names

PHB; PHB1; HEL-215; HEL-S-54e

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Figure 13. IF analysis of STIM1 using anti-STIM1 antibody (AAA19731).STIM1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of STIM1 using anti-STIM1 antibody (AAA19731).STIM1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: rat PC-12 whole cell lysates,Lane 5: mouse liver tissue lysates,Lane 6: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STIM1 antigen affinity purified polyclonal antibody (#AAA19731) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STIM1 at approximately 85 kDa. The expected band size for STIM1 is at 77 kDa.)

STIM1, Polyclonal Antibody (Cat# AAA19731)

Full Name

Anti-STIM1 Antibody Picoband

Gene Names

STIM1; GOK; TAM; TAM1; IMD10; STRMK; D11S4896E

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

TNF alpha, ELISA Kit (Cat# AAA17896)

Full Name

Human TNF alpha ELISA Kit

Gene Names

TNF; DIF; TNFA; TNFSF2; TNF-alpha

Reactivity

Human

Applications

Sandwich ELISA

Pricing

HA1 (A/California/06/09)(H1N1), Recombinant Protein (Cat# AAA13762)

Full Name

HA1 (aa 18-344)(A/California/06/09)(H1N1)

Applications

Western Blot

Purity

= or > 95% purity (SDS PAGE)

Pricing

HA (B/Brisbane/60/2008), Recombinant Protein (Cat# AAA13758)

Full Name

HA (B/Brisbane/60/2008)(aa 16-546)

Applications

Western Blot

Purity

> or = 95% purity (SDS-PAGE)

Pricing

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Application Data (Detection limit for recombinant GST tagged SMAD2 is approximately 0.1ng/ml as a capture antibody.)

SMAD2, Monoclonal Antibody (Cat# AAA25896)

Full Name

SMAD2 (SMAD Family Member 2, JV18, JV18-1, MADH2, MADR2, MGC22139, MGC34440, hMAD-2, hSMAD2) (AP)

Gene Names

SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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Mucin 5 Subtype B (MUC5B), Recombinant Protein (Cat# AAA20203)

Full Name

Recombinant Mucin 5 Subtype B (MUC5B)

Gene Names

MUC5B; MG1; MUC5; MUC9; MUC-5B

Reactivity

Homo sapiens (Human)

Applications

Western Blot

Purity

> 95%

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HEPA1-6 cells using anti-PI-16/PI16 antibody (AAA19330).Overlay histogram showing HEPA1-6 cells stained with AAA19330 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PI-16/PI16 Antibody (AAA19330, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PI-16/PI16, Polyclonal Antibody (Cat# AAA19330)

Full Name

Anti-PI-16/PI16 Antibody

Gene Names

PI16; PSPBP; CRISP9; MSMBBP

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HL-60 cells using anti-PAP/ACP3 antibody (AAA19446).Overlay histogram showing HL-60 cells stained with AAA19446 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAP/ACP3 Antibody (AAA19446, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PAP/ACP3, Polyclonal Antibody (Cat# AAA19446)

Full Name

Anti-PAP/ACP3 Antibody Picoband

Gene Names

ACPP; ACP3; 5'-NT; ACP-3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (HSPA1B monoclonal antibody Western Blot analysis of HSPA1B expression in Raw 264.7.)

HSPA1A, Monoclonal Antibody (Cat# AAA25129)

Full Name

HSPA1A (Heat Shock 70kD Protein 1A/1B, Heat Shock 70kD Protein 1/2, HSP70.1/HSP70.2, HSP70-1/HSP70-2, HSPA1, HSPA1B) (FITC)

Gene Names

HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of SiHa cells using anti-ETFA antibody (AAA19554).Overlay histogram showing SiHa cells stained with AAA19554 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ETFA Antibody (AAA19554, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ETFA, Polyclonal Antibody (Cat# AAA19554)

Full Name

Anti-ETFA Antibody Picoband

Gene Names

ETFA; EMA; GA2; MADD

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

p24 (HIV-1/HXBc2), 6xHis tagged p24 protein, Recombinant Protein (Cat# AAA13756)

Full Name

p24 (HIV-1/HXBc2), 6xHis tagged p24 protein

Applications

Western Blot

Purity

> 95% (SDS-PAGE)

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U20S cells using anti-LRRN4 antibody (AAA20002).Overlay histogram showing U20S cells stained with AAA20002 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LRRN4 Antibody (AAA20002, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of LRRN4 using anti-LRRN4 antibody (AAA20002).LRRN4 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRN4 Antibody (AAA20002) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRN4 Antibody (AAA20002) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRN4 Antibody (AAA20002) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human U20S whole cell lysates,Lane 3: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRN4 antigen affinity purified polyclonal antibody (#AAA20002) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LRRN4 at approximately 79 kDa. The expected band size for LRRN4 is at 79 kDa.)

LRRN4, Polyclonal Antibody (Cat# AAA20002)

Full Name

Anti-LRRN4 Antibody Picoband

Gene Names

LRRN4; NLRR4; NLRR-4; C20orf75; dJ1056H1.1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

HA1 (H1N1) (A/WS/33), Recombinant Protein (Cat# AAA13747)

Full Name

HA1 (H1N1) (aa 18-343) (A/WS/33)

Applications

Western Blot

Purity

> 95% pure by 10%(SDS-PAGE) gel.

Pricing

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WB (Western Blot) (Western Blot analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody. Lane 1: B2M transfected lysate (13.7kD). Lane 2: Non-transfected lysate.)

b2-Microglobulin, Monoclonal Antibody (Cat# AAA25457)

Full Name

b2-Microglobulin (B2M, Beta-2-microglobulin) (HRP)

Gene Names

B2M; IMD43

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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CD274/PD-L1/B7-H1, Recombinant Protein (Cat# AAA18908)

Full Name

Recombinant Human CD274/PD-L1/B7-H1, C-His

Gene Names

CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Applications

Western Blot

Purity

>90% as determined by SDS-PAGE

Pricing

< >

Telomerase Reverse Transcriptase (TERT), Recombinant Protein (Cat# AAA20275)

Full Name

Recombinant Telomerase Reverse Transcriptase (TERT)

Gene Names

Tert; TR; TP2; TRT; EST2; TCS1

Reactivity

Mus musculus (Mouse)

Applications

Western Blot

Purity

> 80%

Pricing

MDM2, Recombinant Protein (Cat# AAA14244)

Full Name

MDM2 (1-118) recombinant protein

Gene Names

MDM2; HDMX; hdm2; ACTFS

Applications

Western Blot

Purity

>90%

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of THP-1 cells using anti-IKZF3 antibody (AAA19753).Overlay histogram showing THP-1 cells stained with AAA19753 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKZF3 Antibody (AAA19753, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of IKZF3 using anti-IKZF3 antibody (AAA19753) and anti-Beta Tubulin antibody (M01857-3).IKZF3 was detected in immunocytochemical section of SIHA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IKZF3 Antibody (AAA19753) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IKZF3 Antibody (AAA19753) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IKZF3 Antibody (AAA19753) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Ramos whole cell lysates,Lane 3: mouse thymus tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKZF3 antigen affinity purified polyclonal antibody (#AAA19753) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IKZF3 at approximately 70 kDa. The expected band size for IKZF3 is at 58 kDa.)

IKZF3, Polyclonal Antibody (Cat# AAA19753)

Full Name

Anti-IKZF3 Antibody Picoband

Gene Names

IKZF3; AIO; AIOLOS; ZNFN1A3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

SFN, Polyclonal Antibody (Cat# AAA10757)

Full Name

SFN Polyclonal Antibody

Gene Names

SFN; YWHAS

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using GAPDH Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

GAPDH, Polyclonal Antibody (Cat# AAA28397)

Full Name

GAPDH Rabbit pAb

Gene Names

GAPDH; G3PD; GAPD

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity purification

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U251 cells using anti-Acid Phosphatase/ACP5 antibody (AAA19481).Overlay histogram showing U251 cells stained with AAA19481 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Acid Phosphatase/ACP5 Antibody (AAA19481, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Acid phosphatase/ACP5, Polyclonal Antibody (Cat# AAA19481)

Full Name

Anti-Acid phosphatase/ACP5 Antibody Picoband

Gene Names

ACP5; TRAP; SPENCDI

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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Growth Arrest Specific Protein 6 (GAS6), Recombinant Protein (Cat# AAA20266)

Full Name

Recombinant Growth Arrest Specific Protein 6 (GAS6)

Reactivity

Rattus norvegicus (Rat)

Applications

Western Blot

Purity

> 97%

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of JK cells using anti-TDP-43/TARDBP antibody (AAA19743).Overlay histogram showing JK cells stained with AAA19743 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TDP-43/TARDBP Antibody (AAA19743, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of TDP-43/TARDBP using anti-TDP-43/TARDBP antibody (AAA19743) and anti-Beta Tubulin antibody (M01857-3).TDP-43/TARDBP was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: rat C6 whole cell lysates,Lane 5: mouse pancrease lysates,Lane 6: mouse Neuro-2a whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDP-43/TARDBP antigen affinity purified polyclonal antibody (#AAA19743) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TDP-43/TARDBP at approximately 43 kDa. The expected band size for TDP-43/TARDBP is at 45 kDa.)

TDP-43/TARDBP, Polyclonal Antibody (Cat# AAA19743)

Full Name

Anti-TDP-43/TARDBP Antibody Picoband

Gene Names

TARDBP; ALS10; TDP-43

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

IF (Immunofluorescence) (Figure 7. IF analysis of Thymidylate Synthase/TYMS using anti-Thymidylate Synthase/TYMS antibody (AAA19511).Thymidylate Synthase/TYMS was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL rabbit anti-Thymidylate Synthase/TYMS Antibody (AAA19511) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

Thymidylate Synthase/TYMS, Polyclonal Antibody (Cat# AAA19511)

Full Name

Anti-Thymidylate Synthase/TYMS Antibody Picoband

Gene Names

TYMS; TS; TMS; HST422

Reactivity

Human, Monkey, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

LOXL1, Recombinant Protein (Cat# AAA14246)

Full Name

LOXL1 recombinant protein

Gene Names

LOXL1; LOL; LOXL

Applications

Western Blot

Purity

>70%

Pricing

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<< Previous Next >>

WB (Western Blot) (Western Blot analysis of FTL expression in transfected 293T cell line by FTL monoclonal antibody (M18), clone X3.Lane 1: FTL transfected lysate(20 KDa).Lane 2: Non-transfected lysate.)

FTL, Monoclonal Antibody (Cat# AAA26147)

Full Name

FTL (Ferritin, Light Polypeptide, MGC71996) (APC)

Gene Names

FTL; LFTD; NBIA3

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-RANBP2 antibody (AAA19742).Overlay histogram showing MCF-7 cells stained with AAA19742 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RANBP2 Antibody (AAA19742, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 8. IF analysis of RANBP2 using anti-RANBP2 antibody (AAA19742).RANBP2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANBP2 antigen affinity purified polyclonal antibody (#AAA19742) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RANBP2 at approximately 358 kDa. The expected band size for RANBP2 is at 358 kDa.)

RANBP2, Polyclonal Antibody (Cat# AAA19742)

Full Name

Anti-RANBP2 Antibody Picoband

Gene Names

RANBP2; ANE1; TRP1; TRP2; ADANE; IIAE3; NUP358

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

TMLHE, Recombinant Protein (Cat# AAA18911)

Full Name

Recombinant Human TMLHE Protein, N-His

Gene Names

TMLHE; TMLD; TMLH; BBOX2; AUTSX6; TMLHED; XAP130

Applications

Western Blot

Purity

>90% as determined by SDS-PAGE

Pricing

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10000 results for "Recombinant Proteins" - showing 50-100

Troponin T Type 1, Slow Skeletal (TNNT1), Recombinant Protein (Cat# AAA20264)

Collagen Type I Alpha 1 (COL1a1), Recombinant Protein (Cat# AAA20234)

Apolipoprotein B100 (APOB100), Recombinant Protein (Cat# AAA21115)

PGP, Polyclonal Antibody (Cat# AAA19740)

Lamin A/C, Monoclonal Antibody (Cat# AAA28306)

Galectin 3/LGALS3, Polyclonal Antibody (Cat# AAA28314)

S100A8, Monoclonal Antibody (Cat# AAA14670)

Selenoprotein P1, Plasma (SEPP1), Recombinant Protein (Cat# AAA20267)

Leptin, ELISA Kit (Cat# AAA30734)

Apolipoprotein B (APOB), Recombinant Protein (Cat# AAA20204)

Vimentin, Monoclonal Antibody (Cat# AAA13829)

MDM2, Monoclonal Antibody (Cat# AAA24860)

MYCBP, Polyclonal Antibody (Cat# AAA19864)

Fetoprotein, Alpha, Monoclonal Antibody (Cat# AAA24127)

Islet Cell Autoantigen 1 (ICA1), Recombinant Protein (Cat# AAA20268)

HA (H7N9) (A/Wuxi/2/2013), Recombinant Protein (Cat# AAA13766)

MAPK8, Polyclonal Antibody (Cat# AAA10635)

b2-Microglobulin, Monoclonal Antibody (Cat# AAA24866)

RPS14, Polyclonal Antibody (Cat# AAA19798)

PPP4C, Polyclonal Antibody (Cat# AAA10744)

E-Cadherin/CD324, Monoclonal Antibody (Cat# AAA23896)

PHB, Monoclonal Antibody (Cat# AAA24609)

STIM1, Polyclonal Antibody (Cat# AAA19731)

TNF alpha, ELISA Kit (Cat# AAA17896)

HA1 (A/California/06/09)(H1N1), Recombinant Protein (Cat# AAA13762)

HA (B/Brisbane/60/2008), Recombinant Protein (Cat# AAA13758)

SMAD2, Monoclonal Antibody (Cat# AAA25896)

Mucin 5 Subtype B (MUC5B), Recombinant Protein (Cat# AAA20203)

PI-16/PI16, Polyclonal Antibody (Cat# AAA19330)

PAP/ACP3, Polyclonal Antibody (Cat# AAA19446)

HSPA1A, Monoclonal Antibody (Cat# AAA25129)

ETFA, Polyclonal Antibody (Cat# AAA19554)

p24 (HIV-1/HXBc2), 6xHis tagged p24 protein, Recombinant Protein (Cat# AAA13756)

LRRN4, Polyclonal Antibody (Cat# AAA20002)

HA1 (H1N1) (A/WS/33), Recombinant Protein (Cat# AAA13747)

b2-Microglobulin, Monoclonal Antibody (Cat# AAA25457)

CD274/PD-L1/B7-H1, Recombinant Protein (Cat# AAA18908)

Telomerase Reverse Transcriptase (TERT), Recombinant Protein (Cat# AAA20275)

MDM2, Recombinant Protein (Cat# AAA14244)

IKZF3, Polyclonal Antibody (Cat# AAA19753)

SFN, Polyclonal Antibody (Cat# AAA10757)

GAPDH, Polyclonal Antibody (Cat# AAA28397)

Acid phosphatase/ACP5, Polyclonal Antibody (Cat# AAA19481)

Growth Arrest Specific Protein 6 (GAS6), Recombinant Protein (Cat# AAA20266)

TDP-43/TARDBP, Polyclonal Antibody (Cat# AAA19743)

Thymidylate Synthase/TYMS, Polyclonal Antibody (Cat# AAA19511)

LOXL1, Recombinant Protein (Cat# AAA14246)

FTL, Monoclonal Antibody (Cat# AAA26147)

RANBP2, Polyclonal Antibody (Cat# AAA19742)

TMLHE, Recombinant Protein (Cat# AAA18911)

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