Search results for "Recombinant Proteins"

HIV-1 p24, Monoclonal Antibody (Cat# AAA13685)

Full Name

Anti-HIV-1 p24

Reactivity

Reacts with P24(HIC-1) proteins

Applications

Western Blot

Purity

Ion exchange column

Pricing

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Application Data (Detection limit for recombinant GST tagged STIP1 is approximately 0.03ng/ml as a capture antibody.)

STIP1, Monoclonal Antibody (Cat# AAA26449)

Full Name

STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (HRP)

Gene Names

STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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WB (Western Blot) (KIF2C monoclonal antibody Western Blot analysis of KIF2C expression in HeLa NE.)

Kinesin 2C, Monoclonal Antibody (Cat# AAA24549)

Full Name

Kinesin 2C (KIF2C, Kinesein Family Member 2C, Kinesin-Like Protein 6, KNSL6, Kinesin-like Protein KIF2C, Mitotic Centromere-Associated Kinesin, MCAK, 4930402F02Rik, ESTM5, MGC11883, OTTHUMP00000010066, X83316) APC

Gene Names

KIF2C; MCAK; CT139; KNSL6

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human stomach using THOC1 at dilution of 1:100 (40x lens).)

THOC1, Polyclonal Antibody (Cat# AAA30702)

Full Name

THOC1 Rabbit Polyclonal Antibody

Gene Names

THOC1; P84; HPR1; P84N5

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A549 cells using anti-Ch TOG/CKAP5 antibody (AAA19386).Overlay histogram showing A549 cells stained with AAA19386 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Ch TOG/CKAP5 Antibody (AAA19386, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ch TOG/CKAP5, Monoclonal Antibody (Cat# AAA19386)

Full Name

Anti-ch TOG/CKAP5 Antibody (monoclonal, 3C13)

Gene Names

CKAP5; TOG; MSPS; TOGp; CHTOG; ch-TOG

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-Asparagine synthetase/ASNS antibody (AAA19789).Overlay histogram showing MCF-7 cells stained with AAA19789 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Asparagine synthetase/ASNS Antibody (AAA19789, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Asparagine synthetase/ASNS using anti-Asparagine synthetase/ASNS antibody (AAA19789).Asparagine synthetase/ASNS was detected in a paraffin-embedded section of human testicular germ cell tumors tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Asparagine synthetase/ASNS Antibody (AAA19789) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Asparagine synthetase/ASNS Antibody (AAA19789) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Asparagine synthetase/ASNS Antibody (AAA19789) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat testis tissue lysates,Lane 6: rat liver tissue lysates,Lane 7: mouse testis tissue lysates,Lane 8: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Asparagine synthetase/ASNS antigen affinity purified polyclonal antibody (#AAA19789) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Asparagine synthetase/ASNS at approximately 64 kDa. The expected band size for Asparagine synthetase/ASNS is at 64 kDa.)

Asparagine synthetase/ASNS, Polyclonal Antibody (Cat# AAA19789)

Full Name

Anti-Asparagine synthetase/ASNS Antibody Picoband

Gene Names

ASNS; TS11; ASNSD

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Raw 264.7.)

ACTB, Monoclonal Antibody (Cat# AAA26656)

Full Name

ACTB (Actin, beta, PS1TP5BP1) (PE)

Gene Names

ACTB; BRWS1; PS1TP5BP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CASP3 antibody.)

CASP3, Monoclonal Antibody (Cat# AAA28234)

Full Name

CASP3 Monoclonal Antibody

Gene Names

CASP3; CPP32; SCA-1; CPP32B

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using TXNL1 antibody. Blue: DAPI for nuclear staining.)

TXNL1, Polyclonal Antibody (Cat# AAA10784)

Full Name

TXNL1 Polyclonal Antibody

Gene Names

TXNL1; Txl; TXNL; TRP32; TXL-1; HEL-S-114

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat brain using RAB11A/RAB11B Rabbit pAb at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

RAB11A/RAB11B, Polyclonal Antibody (Cat# AAA28386)

Full Name

RAB11A/RAB11B Rabbit pAb

Gene Names

RAB11A; YL8

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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Application Data (Detection limit for recombinant GST tagged MECP2 is ~1ng/ml as a capture antibody.)

MECP2, Monoclonal Antibody (Cat# AAA24270)

Full Name

MECP2 (Methyl-CpG-binding Protein 2, MeCp-2 Protein, MeCp2, DKFZp686A24160) (AP)

Gene Names

MECP2; RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of ARID3A over-expressed 293 cell line, cotransfected with ARID3A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ARID3A monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

ARID3A, Monoclonal Antibody (Cat# AAA25322)

Full Name

ARID3A (AT-rich Interactive Domain-containing Protein 3A, ARID Domain-containing Protein 3A, B Cell Regulator of IgH Transcription, Bright, Dead Ringer-like Protein 1, E2F-binding Protein 1, DRIL1, DRIL3, DRX, E2FBP1) (HRP)

Gene Names

ARID3A; DRIL1; DRIL3; BRIGHT; E2FBP1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (KIF2C monoclonal antibody Western Blot analysis of KIF2C expression in HeLa NE.)

Kinesin 2C, Monoclonal Antibody (Cat# AAA25141)

Full Name

Kinesin 2C (KIF2C, Kinesein Family Member 2C, Kinesin-Like Protein 6, KNSL6, Kinesin-like Protein KIF2C, Mitotic Centromere-Associated Kinesin, MCAK, 4930402F02Rik, ESTM5, MGC11883, OTTHUMP00000010066, X83316) (FITC)

Gene Names

KIF2C; MCAK; CT139; KNSL6

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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COX IV, Polyclonal Antibody (Cat# AAA19173)

Full Name

Anti-COX IV Picoband Antibody

Gene Names

COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Immunohistochemistry, Western Blot

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of SH-SY5Y cells using anti-PALM antibody (AAA19727).Overlay histogram showing SH-SY5Y cells stained with AAA19727 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PALM Antibody (AAA19727, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of PALM using anti-PALM antibody (AAA19727).PALM was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PALM Antibody (AAA19727) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PALM Antibody (AAA19727) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PALM Antibody (AAA19727) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PALM Antibody (AAA19727) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PALM Antibody (AAA19727) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PALM antigen affinity purified polyclonal antibody (#AAA19727) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PALM at approximately 37 kDa. The expected band size for PALM is at 42 kDa.)

PALM, Polyclonal Antibody (Cat# AAA19727)

Full Name

Anti-PALM Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of JK cells using anti-LEO1 antibody (AAA19871).Overlay histogram showing JK cells stained with AAA19871 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LEO1 Antibody (AAA19871, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 10. IF analysis of LEO1 using anti-LEO1 antibody (AAA19871).LEO1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LEO1 Antibody (AAA19871) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LEO1 Antibody (AAA19871) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LEO1 Antibody (AAA19871) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LEO1 Antibody (AAA19871) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LEO1 Antibody (AAA19871) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LEO1 Antibody (AAA19871) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LEO1 Antibody (AAA19871) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LEO1 Antibody (AAA19871) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MCF-7 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: human RT4 whole cell lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse C2C12 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LEO1 antigen affinity purified polyclonal antibody (#AAA19871) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LEO1 at approximately 105 kDa. The expected band size for LEO1 is at 75 kDa.)

LEO1, Polyclonal Antibody (Cat# AAA19871)

Full Name

Anti-LEO1 Antibody Picoband

Gene Names

LEO1; RDL

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Figure 15. IF analysis of PHF6 using anti-PHF6 antibody (AAA19786).PHF6 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PHF6 Antibody (AAA19786) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The tissue section was developed using Phalloidin-iFluor 488 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 14. IHC analysis of PHF6 using anti-PHF6 antibody (AAA19786).PHF6 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PHF6 Antibody (AAA19786) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PHF6 Antibody (AAA19786) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PHF6 Antibody (AAA19786) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PHF6 Antibody (AAA19786) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PHF6 Antibody (AAA19786) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PHF6 Antibody (AAA19786) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PHF6 Antibody (AAA19786) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PHF6 Antibody (AAA19786) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PHF6 Antibody (AAA19786) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PHF6 Antibody (AAA19786) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PHF6 Antibody (AAA19786) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PHF6 Antibody (AAA19786) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PHF6 Antibody (AAA19786) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: monkey COS-7 whole cell lysates,Lane 5: rat C6 whole cell lysates,Lane 6: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF6 antigen affinity purified polyclonal antibody (#AAA19786) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PHF6 at approximately 41 kDa. The expected band size for PHF6 is at 41 kDa.)

PHF6, Polyclonal Antibody (Cat# AAA19786)

Full Name

Anti-PHF6 Antibody Picoband

Gene Names

PHF6; BFLS; BORJ; CENP-31

Reactivity

Human, Monkey, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of CACO-2 cells using anti-NEAS/SPTAN1 antibody (AAA19501).Overlay histogram showing CACO-2 cells stained with AAA19501 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEAS/SPTAN1 Antibody (AAA19501, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

NEAS/SPTAN1, Polyclonal Antibody (Cat# AAA19501)

Full Name

Anti-NEAS/SPTAN1 Antibody Picoband

Gene Names

SPTAN1; NEAS; EIEE5; SPTA2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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DUSP6, Polyclonal Antibody (Cat# AAA19449)

Full Name

Anti-DUSP6 Antibody Picoband

Gene Names

DUSP6; MKP3; PYST1

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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Application Data (Detection limit for recombinant GST tagged RPS7 is ~1ng/ml as a capture antibody.)

RPS7, Monoclonal Antibody (Cat# AAA25823)

Full Name

RPS7 (40S Ribosomal Protein S7) (PE)

Gene Names

RPS7; S7; DBA8

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

CLDN6, Recombinant Protein (Cat# AAA11714)

Full Name

Human CLDN6 (138-160) Protein, hFc Tag

Purity

Purified from cell culture supernatant by affinity chromatography
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Pricing

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IHC (Immunohistochemistry) (Figure 7. IHC analysis of PARK7 / DJ1 using anti-PARK7 / DJ1 antibody (AAA19139).PARK7 / DJ1 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PARK7 / DJ1 Antibody (AAA19139) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

PARK7/DJ1, Polyclonal Antibody (Cat# AAA19139)

Full Name

Anti-PARK7/DJ1 Picoband Antibody

Gene Names

Park7; Dj1; CAP1; DJ-1; SP22

Reactivity

Mouse, Rat
No cross reactivity with other proteins.

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse lung using BMP5 antibody at dilution of 1:100 (40x lens).)

BMP5, Polyclonal Antibody (Cat# AAA28209)

Full Name

BMP5 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity Purification

Pricing

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<< Previous Next >>

WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Raw 264.7.)

ACTB, Monoclonal Antibody (Cat# AAA26524)

Full Name

ACTB (Actin, beta, PS1TP5BP1) (HRP)

Gene Names

ACTB; BRWS1; PS1TP5BP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using XRCC4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

XRCC4, Polyclonal Antibody (Cat# AAA10755)

Full Name

XRCC4 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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<< Previous Next >>

WB (Western Blot) (NUP62 monoclonal antibody. Western Blot analysis of NUP62 expression in NIH/3T3.)

NUP62, Monoclonal Antibody (Cat# AAA24592)

Full Name

NUP62 (Nuclear Pore Glycoprotein p62, 62kD Nucleoporin, Nucleoporin Nup62) APC

Gene Names

NUP62; p62; IBSN; SNDI

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using GGA2 antibody. Blue: DAPI for nuclear staining.)

GGA2, Polyclonal Antibody (Cat# AAA10705)

Full Name

GGA2 Polyclonal Antibody

Gene Names

GGA2; VEAR

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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<< Previous Next >>

WB (Western Blot) (Western Blot detection against Immunogen (85.84kD).)

STIP1, Monoclonal Antibody (Cat# AAA25265)

Full Name

STIP1 (Stress-induced-phosphoprotein 1, STI1, Hsc70/Hsp90-organizing Protein, Hop, P60, Renal Carcinoma Antigen NY-REN-11, STI1L, Transformation-sensitive Protein IEF SSP 3521, IEF-SSP-3521) (FITC)

Gene Names

STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human testis tissue using WT1 Rabbit mAb (AAA28568) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WT1, Monoclonal Antibody (Cat# AAA28568)

Full Name

WT1 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 using Heme Oxygenase 1 (HO-1/HMOX1) antibody at dilution of 1 : 50 (40x lens). Blue: DAPI for nuclear staining.)

Heme Oxygenase 1 (HO-1/HMOX1), Polyclonal Antibody (Cat# AAA28414)

Full Name

[KO Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb

Gene Names

NR1D2; RVR; BD73; EAR-1R

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A431 cells using anti-JCHAIN antibody (AAA19779).Overlay histogram showing A431 cells stained with AAA19779 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-JCHAIN Antibody (AAA19779, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of JCHAIN using anti-JCHAIN antibody (AAA19779).JCHAIN was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-JCHAIN Antibody (AAA19779) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-JCHAIN Antibody (AAA19779) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-JCHAIN Antibody (AAA19779) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse spleen tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JCHAIN antigen affinity purified polyclonal antibody (#AAA19779) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for JCHAIN at approximately 23 kDa. The expected band size for JCHAIN is at 18 kDa.)

JCHAIN, Polyclonal Antibody (Cat# AAA19779)

Full Name

Anti-JCHAIN Antibody Picoband

Gene Names

IGJ; JCH; IGCJ

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of THP-1 cells using anti-PELI2 antibody (AAA19939).Overlay histogram showing THP-1 cells stained with AAA19939 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PELI2 Antibody (AAA19939, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of PELI2 using anti-PELI2 antibody (AAA19939).PELI2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI2 Antibody (AAA19939) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI2 Antibody (AAA19939) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI2 Antibody (AAA19939) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Ramos whole cell lysates,Lane 3: rat spleen tissue lysates,Lane 4: rat testis tissue lysates,Lane 5: mouse testis tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PELI2 antigen affinity purified polyclonal antibody (#AAA19939) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PELI2 at approximately 50 kDa. The expected band size for PELI2 is at 46 kDa.)

PELI2, Polyclonal Antibody (Cat# AAA19939)

Full Name

Anti-PELI2 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of K562 cells using anti-ITPA antibody (AAA19773).Overlay histogram showing K562 cells stained with AAA19773 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ITPA Antibody (AAA19773, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of ITPA using anti-ITPA antibody (AAA19773).ITPA was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ITPA Antibody (AAA19773) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ITPA Antibody (AAA19773) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ITPA Antibody (AAA19773) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ITPA Antibody (AAA19773) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ITPA Antibody (AAA19773) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ITPA Antibody (AAA19773) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ITPA Antibody (AAA19773) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ITPA Antibody (AAA19773) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITPA antigen affinity purified polyclonal antibody (#AAA19773) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ITPA at approximately 21 kDa. The expected band size for ITPA is at 21 kDa.)

ITPA, Polyclonal Antibody (Cat# AAA19773)

Full Name

Anti-ITPA Antibody Picoband

Gene Names

ITPA; My049; C20orf37; dJ794I6.3; HLC14-06-P

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of PC-3 cells using anti-Beclin 1 antibody (AAA19664).Overlay histogram showing PC-3 cells stained with AAA19664 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Beclin 1 Antibody (AAA19664, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Beclin 1, Monoclonal Antibody (Cat# AAA19664)

Full Name

Anti-Beclin 1 Antibody Picoband (monoclonal, 2D12A3)

Gene Names

BECN1; ATG6; VPS30; beclin1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

ADAMTS13, ELISA Kit (Cat# AAA27868)

Full Name

Human ADAMTS13 ELISA Kit

Gene Names

ADAMTS13; VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13

Reactivity

Human

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained ES-2 cells (Black) as negative control.)

STIP1, Monoclonal Antibody (Cat# AAA26274)

Full Name

STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (Biotin)

Gene Names

STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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<< Previous Next >>

WB (Western Blot) (BANF1 monoclonal antibody (M07), clone M2. Western Blot analysis of BANF1 expression in PC-12.)

BANF1, Monoclonal Antibody (Cat# AAA26177)

Full Name

BANF1 (Barrier to Autointegration Factor 1, BAF, BCRP1, D14S1460, MGC111161) (Biotin)

Gene Names

BANF1; BAF; NGPS; BCRP1; D14S1460

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

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<< Previous Next >>

WB (Western Blot) (LRRN1 monoclonal antibody (M05), clone 3D11. Western Blot analysis of LRRN1 expression in PC-12.)

LRRN1, Monoclonal Antibody (Cat# AAA26151)

Full Name

LRRN1 (Leucine Rich Repeat Neuronal 1, FIGLER3, KIAA1497, NLRR-1, LRCH4) (APC)

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified

Pricing

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<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse lung using KIF1B Antibody at dilution of 1:100 (40x lens).)

KIF1B, Polyclonal Antibody (Cat# AAA10778)

Full Name

KIF1B Polyclonal Antibody

Gene Names

KIF1B; KLP; CMT2; CMT2A; CMT2A1; HMSNII; NBLST1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

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<< Previous Next >>

WB (Western Blot) (Western Blot analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).Lane 2: Non-transfected lysate.)

MPP1, Monoclonal Antibody (Cat# AAA25946)

Full Name

MPP1 (Membrane Protein, Palmitoylated 1, 55kD, AAG12, DXS552, DXS552E, EMP55, MRG1, PEMP) (AP)

Gene Names

MPP1; MRG1; PEMP; AAG12; EMP55; DXS552E

Applications

Immunoprecipitation, Western Blot

Purity

Purified

Pricing

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of ARID3A over-expressed 293 cell line, cotransfected with ARID3A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ARID3A monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

ARID3A, Monoclonal Antibody (Cat# AAA25026)

Full Name

ARID3A (AT-rich Interactive Domain-containing Protein 3A, ARID Domain-containing Protein 3A, B Cell Regulator of IgH Transcription, Bright, Dead Ringer-like Protein 1, E2F-binding Protein 1, DRIL1, DRIL3, DRX, E2FBP1) (FITC)

Gene Names

ARID3A; DRIL1; DRIL3; BRIGHT; E2FBP1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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<< Previous Next >>

WB (Western Blot) (SMAD3 monoclonal antibody. Western Blot analysis of SMAD3 expression in human colon.)

SMAD3, Monoclonal Antibody (Cat# AAA25544)

Full Name

SMAD3 (Mothers Against Decapentaplegic Homolog 3, SMAD 3, Mothers Against DPP Homolog 3, MAD Homolog 3, Mad3, hMAD-3, SMAD Family Member 3, JV15-2, hSMAD3, MADH3, DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, MGC60396) (HRP)

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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<< Previous Next >>

IHC (Immunohistchemistry) (Figure 5. IHC analysis of GNG4 using anti-GNG4 antibody (AAA19341).GNG4 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG4 Antibody (AAA19341) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

GNG4, Polyclonal Antibody (Cat# AAA19341)

Full Name

Anti-GNG4 Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-ALK-1/ACVRL1 antibody (AAA19242).Overlay histogram showing MCF-7 cells stained with AAA19242 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALK-1/ACVRL1 Antibody (AAA19242, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ALK-1/ACVRL1, Polyclonal Antibody (Cat# AAA19242)

Full Name

Anti-ALK-1/ACVRL1 Antibody

Gene Names

ACVRL1; HHT; ALK1; HHT2; ORW2; SKR3; ALK-1; TSR-I; ACVRLK1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of JK cells using anti-SCP3/SYCP3 antibody (AAA19556).Overlay histogram showing JK cells stained with AAA19556 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCP3/SYCP3 Antibody (AAA19556, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

SCP3/SYCP3, Polyclonal Antibody (Cat# AAA19556)

Full Name

Anti-SCP3/SYCP3 Antibody Picoband

Gene Names

SYCP3; COR1; SCP3; SPGF4

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of A549 cells using anti-CYB5B antibody (AAA19612).Overlay histogram showing A549 cells stained with AAA19612 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYB5B Antibody (AAA19612, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

CYB5B, Polyclonal Antibody (Cat# AAA19612)

Full Name

Anti-CYB5B Antibody Picoband

Gene Names

CYB5B; OMB5; CYB5-M; CYPB5M

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of MCF-7 cells using anti-Kinectin 1/KTN1 antibody (AAA19534).Overlay histogram showing MCF-7 cells stained with AAA19534 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Kinectin 1/KTN1 Antibody (AAA19534, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Kinectin 1/KTN1, Polyclonal Antibody (Cat# AAA19534)

Full Name

Anti-Kinectin 1/KTN1 Antibody Picoband

Gene Names

KTN1; CG1; KNT; MU-RMS-40.19

Reactivity

Human, Mouse, Rat, Monkey

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

TGF beta 1, ELISA Kit (Cat# AAA17876)

Full Name

Rat TGF beta 1 ELISA Kit

Gene Names

Tgfb1; Tgfb

Reactivity

Rat

Applications

Sandwich ELISA

Pricing

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<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using PDYN antibody at dilution of 1:100 (40x lens).)

PDYN, Polyclonal Antibody (Cat# AAA10782)

Full Name

PDYN

Gene Names

PDYN; ADCA; PENKB; SCA23

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).Lane 2: Non-transfected lysate.)

MPP1, Monoclonal Antibody (Cat# AAA26610)

Full Name

MPP1 (Membrane Protein, Palmitoylated 1, 55kD, AAG12, DXS552, DXS552E, EMP55, MRG1, PEMP) (PE)

Gene Names

MPP1; MRG1; PEMP; AAG12; EMP55; DXS552E

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified

Pricing

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10000 results for "Recombinant Proteins" - showing 500-550

HIV-1 p24, Monoclonal Antibody (Cat# AAA13685)

STIP1, Monoclonal Antibody (Cat# AAA26449)

Kinesin 2C, Monoclonal Antibody (Cat# AAA24549)

THOC1, Polyclonal Antibody (Cat# AAA30702)

ch TOG/CKAP5, Monoclonal Antibody (Cat# AAA19386)

Asparagine synthetase/ASNS, Polyclonal Antibody (Cat# AAA19789)

ACTB, Monoclonal Antibody (Cat# AAA26656)

CASP3, Monoclonal Antibody (Cat# AAA28234)

TXNL1, Polyclonal Antibody (Cat# AAA10784)

RAB11A/RAB11B, Polyclonal Antibody (Cat# AAA28386)

MECP2, Monoclonal Antibody (Cat# AAA24270)

ARID3A, Monoclonal Antibody (Cat# AAA25322)

Kinesin 2C, Monoclonal Antibody (Cat# AAA25141)

COX IV, Polyclonal Antibody (Cat# AAA19173)

PALM, Polyclonal Antibody (Cat# AAA19727)

LEO1, Polyclonal Antibody (Cat# AAA19871)

PHF6, Polyclonal Antibody (Cat# AAA19786)

NEAS/SPTAN1, Polyclonal Antibody (Cat# AAA19501)

DUSP6, Polyclonal Antibody (Cat# AAA19449)

RPS7, Monoclonal Antibody (Cat# AAA25823)

CLDN6, Recombinant Protein (Cat# AAA11714)

PARK7/DJ1, Polyclonal Antibody (Cat# AAA19139)

BMP5, Polyclonal Antibody (Cat# AAA28209)

ACTB, Monoclonal Antibody (Cat# AAA26524)

XRCC4, Polyclonal Antibody (Cat# AAA10755)

NUP62, Monoclonal Antibody (Cat# AAA24592)

GGA2, Polyclonal Antibody (Cat# AAA10705)

STIP1, Monoclonal Antibody (Cat# AAA25265)

WT1, Monoclonal Antibody (Cat# AAA28568)

Heme Oxygenase 1 (HO-1/HMOX1), Polyclonal Antibody (Cat# AAA28414)

JCHAIN, Polyclonal Antibody (Cat# AAA19779)

PELI2, Polyclonal Antibody (Cat# AAA19939)

ITPA, Polyclonal Antibody (Cat# AAA19773)

Beclin 1, Monoclonal Antibody (Cat# AAA19664)

ADAMTS13, ELISA Kit (Cat# AAA27868)

STIP1, Monoclonal Antibody (Cat# AAA26274)

BANF1, Monoclonal Antibody (Cat# AAA26177)

LRRN1, Monoclonal Antibody (Cat# AAA26151)

KIF1B, Polyclonal Antibody (Cat# AAA10778)

MPP1, Monoclonal Antibody (Cat# AAA25946)

ARID3A, Monoclonal Antibody (Cat# AAA25026)

SMAD3, Monoclonal Antibody (Cat# AAA25544)

GNG4, Polyclonal Antibody (Cat# AAA19341)

ALK-1/ACVRL1, Polyclonal Antibody (Cat# AAA19242)

SCP3/SYCP3, Polyclonal Antibody (Cat# AAA19556)

CYB5B, Polyclonal Antibody (Cat# AAA19612)

Kinectin 1/KTN1, Polyclonal Antibody (Cat# AAA19534)

TGF beta 1, ELISA Kit (Cat# AAA17876)

PDYN, Polyclonal Antibody (Cat# AAA10782)

MPP1, Monoclonal Antibody (Cat# AAA26610)

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CASP3, Monoclonal Antibody (Cat# AAA28234)