Search results for "Recombinant Proteins"

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IF (Immunofluorescence) (Figure 8. IF analysis of GCLC using anti-GCLC antibody (AAA19759).GCLC was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-GCLC Antibody (AAA19759) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of GCLC using anti-GCLC antibody (AAA19759).GCLC was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GCLC Antibody (AAA19759) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GCLC Antibody (AAA19759) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GCLC Antibody (AAA19759) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GCLC Antibody (AAA19759) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human A431 whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: rat C6 whole cell lysates,Lane 6: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCLC antigen affinity purified polyclonal antibody (#AAA19759) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GCLC at approximately 73 kDa. The expected band size for GCLC is at 73 kDa.)

GCLC, Polyclonal Antibody (Cat# AAA19759)

Full Name

Anti-GCLC Antibody Picoband

Gene Names

GCLC; GCL; GCS; GLCL; GLCLC

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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Application Data (Detection limit for recombinant GST tagged RPS7 is ~1ng/ml as a capture antibody.)

RPS7, Monoclonal Antibody (Cat# AAA24641)

Full Name

RPS7 (40S Ribosomal Protein S7) APC

Gene Names

RPS7; S7; DBA8

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (RRAS2 monoclonal antibody Western Blot analysis of RRAS2 expression in A-431.)

RRAS2, Monoclonal Antibody (Cat# AAA24642)

Full Name

RRAS2 (Ras-related Protein R-Ras2, Ras-like Protein TC21, Teratocarcinoma Oncogene, TC21) APC

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 100ug extracts of 293T cells using 3ug DDX1 antibody. Western blot was performed from the immunoprecipitate using DDX1 antibody at a dilition of 1:1000.)

DDX1, Polyclonal Antibody (Cat# AAA28165)

Full Name

DDX1 Polyclonal Antibody

Gene Names

DDX1; DBP-RB; UKVH5d

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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WB (Western Blot) (G3BP monoclonal antibody Western Blot analysis of G3BP expression in A-431)

G3BP1, Monoclonal Antibody (Cat# AAA24809)

Full Name

G3BP1 (Ras GTPase-activating Protein-binding Protein 1, G3BP-1, ATP-dependent DNA Helicase VIII, hDH VIII, GAP SH3 Domain-binding Protein 1, G3BP) (Biotin)

Gene Names

G3BP1; G3BP; HDH-VIII

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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ECHS1, Polyclonal Antibody (Cat# AAA19519)

Full Name

Anti-ECHS1 Antibody Picoband

Gene Names

ECHS1; SCEH

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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TOMM40, Polyclonal Antibody (Cat# AAA19478)

Full Name

Anti-TOMM40 Antibody Picoband

Gene Names

TOMM40; TOM40; PEREC1; C19orf1; PER-EC1; D19S1177E

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using SEPT7 antibody.)

SEPT7, Polyclonal Antibody (Cat# AAA10767)

Full Name

SEPT7 Polyclonal Antibody

Gene Names

SEPT7; CDC3; CDC10; SEPT7A; NBLA02942

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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Application Data (Detection limit for recombinant GST tagged PCNA is ~0.1ng/ml as a capture antibody.)

PCNA, Monoclonal Antibody (Cat# AAA24896)

Full Name

PCNA (Proliferating Cell Nuclear Antigen, Cyclin, MGC8367) (Biotin)

Gene Names

PCNA; ATLD2

Reactivity

Human

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (RPS6KB1 monoclonal antibody (M03), clone 2C2. Western Blot analysis of RPS6KB1 expression in PC-12.)

RPS6KB1, Monoclonal Antibody (Cat# AAA26103)

Full Name

RPS6KB1 (Ribosomal Protein S6 Kinase, 70kD, Polypeptide 1, PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-S6K, p70-alpha) (APC)

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

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HMGB1, Polyclonal Antibody (Cat# AAA10718)

Full Name

HMGB1 Polyclonal Antibody

Gene Names

HMGB1; HMG1; HMG3; SBP-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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HSP90AB1, Polyclonal Antibody (Cat# AAA10729)

Full Name

HSP90AB1 Polyclonal Antibody

Gene Names

HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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Application Data (Detection limit for recombinant GST tagged MECP2 is ~1ng/ml as a capture antibody.)

MECP2, Monoclonal Antibody (Cat# AAA24566)

Full Name

MECP2 (Methyl-CpG-binding Protein 2, MeCp-2 Protein, MeCp2, DKFZp686A24160) APC

Gene Names

MECP2; RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Detection limit for recombinant GST tagged PTBP1 is ~0.1ng/ml as a capture antibody.)

PTBP1, Monoclonal Antibody (Cat# AAA24627)

Full Name

PTBP1 (Polypyrimidine Tract-binding Protein 1, PTB, Heterogeneous Nuclear Ribonucleoprotein I, hnRNP I, 57kD RNA-binding Protein PPTB-1, MGC10830, MGC8461) APC

Gene Names

PTBP1; PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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COX1/Cyclooxygenase 1, Monoclonal Antibody (Cat# AAA30153)

Full Name

COX1/Cyclooxygenase 1 Antibody

Gene Names

PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Purity

ProA affinity purified

Pricing

IHC (Immunohistochemistry) (Figure 8. IHC analysis of Rab4/RAB4A using anti-Rab4/RAB4A antibody (AAA19822).Rab4/RAB4A was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab4/RAB4A antigen affinity purified polyclonal antibody (#AAA19822) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Rab4/RAB4A at approximately 24 kDa. The expected band size for Rab4/RAB4A is at 24 kDa.)

Rab4/RAB4A, Polyclonal Antibody (Cat# AAA19822)

Full Name

Anti-Rab4/RAB4A Antibody Picoband

Gene Names

RAB4A; RAB4; HRES1; HRES-1; HRES-1/RAB4

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Figure 6. IF analysis of Filamin B/FLNB using anti-Filamin B/FLNB antibody (AAA19696).Filamin B/FLNB was detected in an immunocytochemical section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-Filamin B/FLNB Antibody (AAA19696) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

Filamin B/FLNB, Monoclonal Antibody (Cat# AAA19696)

Full Name

Anti-Filamin B/FLNB Antibody Picoband (monoclonal, 11E2D2)

Gene Names

FLNB; AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of JK cells using anti-Golgin 97/GOLGA1 antibody (AAA19636).Overlay histogram showing JK cells stained with AAA19636 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Golgin 97/GOLGA1 Antibody (AAA19636, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Golgin 97/GOLGA1, Polyclonal Antibody (Cat# AAA19636)

Full Name

Anti-Golgin 97/GOLGA1 Antibody Picoband

Gene Names

GOLGA1; golgin-97

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HeLa cells using anti-RPL23 antibody (AAA19565).Overlay histogram showing HeLa cells stained with AAA19565 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL23 Antibody (AAA19565, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

RPL23, Polyclonal Antibody (Cat# AAA19565)

Full Name

Anti-RPL23 Antibody Picoband

Gene Names

RPL23; L23; rpL17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HeLa cells using anti-delta 1 Catenin/CAS/CTNND1 antibody (AAA19453).Overlay histogram showing HeLa cells stained with AAA19453 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-delta 1 Catenin/CAS/CTNND1 Antibody (AAA19453, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

delta 1 Catenin/CAS/CTNND1, Polyclonal Antibody (Cat# AAA19453)

Full Name

Anti-delta 1 Catenin/CAS/CTNND1 Antibody Picoband

Gene Names

CTNND1; CAS; p120; CTNND; P120CAS; P120CTN; p120(CAS); p120(CTN)

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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KLHL12, Polyclonal Antibody (Cat# AAA19601)

Full Name

Anti-KLHL12 Antibody Picoband

Gene Names

KLHL12; DKIR; C3IP1

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HEL cells using anti-NDUFB8 antibody (AAA19591).Overlay histogram showing HEL cells stained with AAA19591 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFB8 Antibody (AAA19591, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

NDUFB8, Polyclonal Antibody (Cat# AAA19591)

Full Name

Anti-NDUFB8 Antibody Picoband

Gene Names

NDUFB8; ASHI; CI-ASHI

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

COMP, ELISA Kit (Cat# AAA17873)

Full Name

Human COMP ELISA Kit

Gene Names

COMP; MED; EDM1; EPD1; PSACH; THBS5

Reactivity

Human

Applications

Sandwich ELISA

Pricing

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KRT20, Polyclonal Antibody (Cat# AAA10645)

Full Name

KRT20 Polyclonal Antibody

Gene Names

KRT20; K20; CD20; CK20; CK-20; KRT21

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using GRSF1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

GRSF1, Polyclonal Antibody (Cat# AAA28367)

Full Name

GRSF1 Rabbit pAb

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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HNRNPD, Polyclonal Antibody (Cat# AAA28104)

Full Name

HNRNPD Polyclonal Antibody

Gene Names

HNRNPD; P37; AUF1; AUF1A; HNRPD; hnRNPD0

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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WB (Western Blot) (EFHD1 monoclonal antibody (M05), Western Blot analysis of EFHD1 expression in HeLa.)

EFHD1, Monoclonal Antibody (Cat# AAA25378)

Full Name

EFHD1 (EF-hand Domain-containing Protein D1, EF-hand Domain-containing Protein 1, Swiprosin-2, SWS2, PP3051) (HRP)

Gene Names

EFHD1; SWS2; MST133; PP3051; MSTP133; FLJ13612; DKFZp781H0842

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of GALE using anti-GALE antibody (AAA19135).GALE was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GALE Antibody (AAA19135) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

GALE, Polyclonal Antibody (Cat# AAA19135)

Full Name

Anti-GALE Picoband antibody

Gene Names

GALE; SDR1E1

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Immunohistochemistry, Western Blot

Pricing

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Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using CAIX-Monospecific Mouse Monoclonal Antibody (CA9/3406). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Renal Cell Carcinoma, Monoclonal Antibody (Cat# AAA23952)

Full Name

Renal Cell Carcinoma (Carbonic Anhydrase IX)

Gene Names

CA9; MN; CAIX

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U87 cells using anti-GRB10 antibody (AAA19244).Overlay histogram showing U87 cells stained with AAA19244 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRB10 Antibody (AAA19244,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

GRB10, Polyclonal Antibody (Cat# AAA19244)

Full Name

Anti-GRB10 Antibody

Gene Names

GRB10; RSS; IRBP; MEG1; GRB-IR; Grb-10

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of Raji cells using anti-PSMC4 antibody (AAA19860).Overlay histogram showing Raji cells stained with AAA19860 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMC4 Antibody (AAA19860, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of PSMC4 using anti-PSMC4 antibody (AAA19860) and anti-Beta Tubulin antibody (M01857-3).PSMC4 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSMC4 Antibody (AAA19860) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSMC4 Antibody (AAA19860) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSMC4 Antibody (AAA19860) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSMC4 Antibody (AAA19860) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSMC4 Antibody (AAA19860) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human U251 whole cell lysates,Lane 3: human U251 whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC4 antigen affinity purified polyclonal antibody (#AAA19860) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PSMC4 at approximately 50 kDa. The expected band size for PSMC4 is at 43-50 kDa.)

PSMC4, Polyclonal Antibody (Cat# AAA19860)

Full Name

Anti-PSMC4 Antibody Picoband

Gene Names

PSMC4; S6; RPT3; TBP7; TBP-7; MIP224

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of Daudi cells using anti-HLA-DRA antibody (AAA19680).Overlay histogram showing Daudi cells stained with AAA19680 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HLA-DRA Antibody (AAA19680, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

HLA-DR/HLA-DRA, Monoclonal Antibody (Cat# AAA19680)

Full Name

Anti-HLA-DR/HLA-DRA Antibody Picoband (monoclonal, 8I10H1)

Gene Names

HLA-DRA; MLRW; HLA-DRA1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of CACO-2 cells using anti-BRSK1 antibody (AAA19562).Overlay histogram showing CACO-2 cells stained with AAA19562 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRSK1 Antibody (AAA19562, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

BRSK1, Polyclonal Antibody (Cat# AAA19562)

Full Name

Anti-BRSK1 Antibody Picoband

Gene Names

BRSK1; hSAD1

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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PC4/SUB1, Monoclonal Antibody (Cat# AAA19701)

Full Name

Anti-PC4/SUB1 Antibody Picoband (monoclonal, 6B5B10)

Gene Names

SUB1; P15; PC4; p14

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human stomach using SDC2 antibody at dilution of 1:100 (40x lens).)

SDC2, Polyclonal Antibody (Cat# AAA10698)

Full Name

SDC2 Polyclonal Antibody

Gene Names

SDC2; HSPG; CD362; HSPG1; SYND2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

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Application Data (Detection limit for recombinant GST tagged DDX56 is 0.1 ng/ml as a capture antibody.)

DDX56, Monoclonal Antibody (Cat# AAA25987)

Full Name

DDX56 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 56, DDX21, DDX26, NOH61) (AP)

Gene Names

DDX56; DDX21; DDX26; NOH61

Applications

Western Blot

Purity

Purified

Pricing

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TOMM20, Polyclonal Antibody (Cat# AAA28123)

Full Name

TOMM20 Polyclonal Antibody

Gene Names

TOMM20; MAS20; MOM19; TOM20

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity Purification

Pricing

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WB (Western Blot) (G3BP monoclonal antibody Western Blot analysis of G3BP expression in A-431)

G3BP1, Monoclonal Antibody (Cat# AAA24514)

Full Name

G3BP1 (Ras GTPase-activating Protein-binding Protein 1, G3BP-1, ATP-dependent DNA Helicase VIII, hDH VIII, GAP SH3 Domain-binding Protein 1, G3BP) APC

Gene Names

G3BP1; G3BP; HDH-VIII

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

HSPB1, Monoclonal Antibody (Cat# AAA24539)

Full Name

HSPB1 (Heat Shock Protein beta-1, HspB1, Heat Shock 27kD Protein, HSP 27, Stress-responsive Protein 27, SRP27, Estrogen-regulated 24kD Protein, 28kD Heat Shock Protein, HSPB1, HSP27) APC

Gene Names

HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102

Reactivity

Human

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of MAP1LC3A using anti-MAP1LC3A antibody (AAA19151).MAP1LC3A was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP1LC3A Antibody (AAA19151) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

MAP1LC3A, Polyclonal Antibody (Cat# AAA19151)

Full Name

Anti-MAP1LC3A Picoband antibody

Gene Names

MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Immunohistochemistry, Western Blot

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of CACO-2 cells using anti-Glycine decarboxylase/GLDC antibody (AAA19300).Overlay histogram showing CACO-2 cells stained with AAA19300 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Glycine decarboxylase/GLDC Antibody (AAA19300, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Glycine decarboxylase/GLDC, Polyclonal Antibody (Cat# AAA19300)

Full Name

Anti-Glycine decarboxylase/GLDC Antibody

Gene Names

GLDC; GCE; GCSP; HYGN1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of THP-1 cells using anti-S100A4 antibody (AAA19235).Overlay histogram showing THP-1 cells stained with AAA19235 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A4 Antibody (AAA19235, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

S100A4, Polyclonal Antibody (Cat# AAA19235)

Full Name

Anti-S100A4 Antibody

Gene Names

S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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CDC123, Polyclonal Antibody (Cat# AAA19323)

Full Name

Anti-CDC123 Antibody

Gene Names

CDC123; D123; C10orf7

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HCT116 cells using anti-SRPRA antibody (AAA19972).Overlay histogram showing HCT116 cells stained with AAA19972 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRPRA Antibody (AAA19972, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 4. IF analysis of SRPRA using anti-SRPRA antibody (AAA19972).SRPRA was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SRPRA Antibody (AAA19972) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SRPRA Antibody (AAA19972) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human HEL whole cell lysates,Lane 4: rat liver tissue lysates,Lane 5: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRPRA antigen affinity purified polyclonal antibody (#AAA19972) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SRPRA at approximately 70 kDa. The expected band size for SRPRA is at 70 kDa.)

SRPRA, Polyclonal Antibody (Cat# AAA19972)

Full Name

Anti-SRPRA Antibody Picoband

Gene Names

SRPR; DP; Sralpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HEL cells using anti-GNAI2 antibody (AAA19777).Overlay histogram showing HEL cells stained with AAA19777 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GNAI2 Antibody (AAA19777, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of GNAI2 using anti-GNAI2 antibody (AAA19777).GNAI2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GNAI2 Antibody (AAA19777) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GNAI2 Antibody (AAA19777) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GNAI2 Antibody (AAA19777) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GNAI2 Antibody (AAA19777) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GNAI2 Antibody (AAA19777) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GNAI2 Antibody (AAA19777) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HEL whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNAI2 antigen affinity purified polyclonal antibody (#AAA19777) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GNAI2 at approximately 40 kDa. The expected band size for GNAI2 is at 40 kDa.)

GNAI2, Polyclonal Antibody (Cat# AAA19777)

Full Name

Anti-GNAI2 Antibody Picoband

Gene Names

GNAI2; GIP; GNAI2B; H_LUCA15.1; H_LUCA16.1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Figure 7. IF analysis of LSR using anti-LSR antibody (AAA19467).LSR was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-LSR Antibody (AAA19467) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)