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838 results for " Purified Polyclonal Antibodies" - showing 800-838
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26895)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
ICC, EIA, WB, IP, IHC
Purity
Purified by Immunoaffinity chromatography.
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26901)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
ICC, WB, IP, IHC
Purity
Purified by Immunoaffinity chromatography.
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26899)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
ICC, WB, IP, IHC
Purity
Purified by Immunoaffinity chromatography.
Pricing
WB (Western Blot)
(WB Suggested Anti-PSEN1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human brain)
PSEN1, Polyclonal Antibody (Cat# AAA23585)
Full Name
PSEN1 antibody - middle region
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
IHC, IP, WB
Purity
Affinity Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation(IP) of CRYAB by using monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-CRYAB antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.)
CRYAB / Alpha B Crystallin, Monoclonal Antibody (Cat# AAA12373)
Full Name
Mouse Monoclonal [clone 6D11] (IgG1) to Human CRYAB / Alpha B Crystallin
Gene Names
CRYAB; MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
Reactivity
Human, Monkey, Rat
Applications
IHC - Paraffin, IF, WB, IP, FC/FACS
Purity
Protein A/G Purified
Pricing
WB (Western Blot)
(HSPA1B monoclonal antibody Western Blot analysis of HSPA1B expression in Raw 264.7.)
HSPA1A, Monoclonal Antibody (Cat# AAA24537)
Full Name
HSPA1A (Heat Shock 70kD Protein 1A/1B, Heat Shock 70kD Protein 1/2, HSP70.1/HSP70.2, HSP70-1/HSP70-2, HSPA1, HSPA1B) APC
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
Reactivity
Human, Mouse, Rat
Applications
EIA, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).)
FGFR2, Polyclonal Antibody (Cat# AAA26851)
Full Name
FGFR2, NT (FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332) (AP)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, Monkey, Mouse, Rat
Applications
IF, EIA, IHC, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
Application Data
(C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).)
FGFR2, Polyclonal Antibody (Cat# AAA26855)
Full Name
FGFR2, NT (FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332) (Azide free) (HRP)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, Monkey, Mouse, Rat
Applications
IHC, EIA, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
Application Data
(C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).)
FGFR2, Polyclonal Antibody (Cat# AAA26853)
Full Name
FGFR2, NT (FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332) (Biotin)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, Monkey, Mouse, Rat
Applications
FC/FACS, EIA, IF, IHC, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
WB (Western Blot)
(Figure 9 KD Validation of PUMA (Han et al., 2010)Immunoblot analyses of Tet-induced p53 cells treated with NOXA, Puma, Bim or non-targeting siRNAs that were utilized in this experiment. PUMA protein levels were markedly reduced in PUMA KD cells detected by anti-PUMA antibodies (3041).)
PUMA, Polyclonal Antibody (Cat# AAA10943)
Full Name
PUMA Antibody
Gene Names
BBC3; JFY1; PUMA; JFY-1
Reactivity
Human, Mouse
Applications
EIA, WB, ICC, IF
Purity
PUMA Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)
OPN-a/b, Polyclonal Antibody (Cat# AAA26861)
Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (MaxLight 490)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
WB, IHC, IF
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)
OPN-a/b, Polyclonal Antibody (Cat# AAA26865)
Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (PE)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
WB, IHC, IF
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
FCM (Flow Cytometry)
(GTF2I Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
GTF2I, Polyclonal Antibody (Cat# AAA28707)
Full Name
GTF2I Antibody (C-term)
Gene Names
GTF2I; WBS; DIWS; SPIN; IB291; BAP135; BTKAP1; TFII-I; WBSCR6; GTFII-I
Reactivity
Human (Predicted Reactivity: Rat)
Applications
IF, EIA, IHC, FC/FACS, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IP (Immunoprecipitation)
(Immunoprecipitating GAPDH in Hela whole cell lysateLane 1: Mouse control IgG instead of in Hela whole cell lysate. Lane 2: (5ul) + Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug)For western blotting, the blot was detected at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000)
GAPDH, Monoclonal Antibody (Cat# AAA27042)
Full Name
GAPDH Monoclonal Antibody
Reactivity
Human, Mouse, Rabbit
Applications
EIA, WB, IHC, IP, IF
Purity
>95%, Protein G purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)
OPN-a/b, Polyclonal Antibody (Cat# AAA26858)
Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (Biotin)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
WB, IHC, IF, EIA
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
IP (Immunoprecipitation)
(Figure 11 Immunoprecipitation Validation in HEK293 cells (Kawai et al., 2004)HEK293 cells were transiently transfected with DYKDDDDK-IRF7. Ccell lysates were immunoprecipitated with control rabbit anti-mouse immunoglobulin serum (IgG) or anti-MyD88 (Ab1 and Ab2), followed by immunoblotting with anti-DYKDDDDK.)
MYD88, Polyclonal Antibody (Cat# AAA10944)
Full Name
MYD88 Antibody
Gene Names
MYD88; MYD88D
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Purity
MYD88 Antibody is affinity chromatography purified via peptide column.
Pricing
FCM (Flow Cytometry)
(Overlay Peak curve showing 293F cells transfected with GST stained with AAA28065 (red line) at 1:189. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1?g/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1?g/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
GST, Monoclonal Antibody (Cat# AAA28065)
Full Name
GST Monoclonal Antibody
Reactivity
All
Applications
EIA, WB, IF, FC/FACS, IP
Purity
>95%, Protein A purified
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with XRCC6 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with XRCC6 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). XRCC6 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.)
XRCC6, Polyclonal Antibody (Cat# AAA28768)
Full Name
XRCC6 Antibody (C-term)
Gene Names
XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)
OPN-a/b, Polyclonal Antibody (Cat# AAA26857)
Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (APC)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
WB, IHC, IF
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)
OPN-a/b, Polyclonal Antibody (Cat# AAA26860)
Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (MaxLight 405)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
WB, IHC, IF
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)
OPN-a/b, Polyclonal Antibody (Cat# AAA26859)
Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (FITC)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
WB, IHC, IF
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)
OPN-a/b, Polyclonal Antibody (Cat# AAA26864)
Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (MaxLight 750)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
WB, IHC, IF
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)
OPN-a/b, Polyclonal Antibody (Cat# AAA26863)
Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (MaxLight 650)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
WB, IHC, IF
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)
OPN-a/b, Polyclonal Antibody (Cat# AAA26862)
Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (MaxLight 550)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
WB, IHC, IF
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
FCM (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA28066 (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1?g/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1?g/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
ACTB, Monoclonal Antibody (Cat# AAA28066)
Full Name
ACTB Monoclonal Antibody
Reactivity
Human, Mouse, Rat, Rabbit
Applications
EIA, WB, IHC, IF, FC/FACS, IP
Purity
>95%, Protein A purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitating GAPDH in Hela whole cell lysate Lane 1: Mouse control IgG instead of AAA27043 in Hela whole cell lysate. Lane 2: AAA27043 (5ul) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (10ug) For western blotting, the blot was detected with AAA27043 at 1:5000, and a HRPconjugated Protein G antibody was used as the secondary antibody at 1:2000)
GAPDH, Monoclonal Antibody (Cat# AAA27043)
Full Name
GAPDH Monoclonal Antibody
Reactivity
Human, Rat, Rabbit, Mouse
Applications
EIA, WB, IHC, IP, IF
Purity
>95%, Protein G purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling DLL3 with (1:25), followed by Dylight 488- conjugated goat anti- rabbit IgG secondary antibody at (1:200) (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on U-2 OScell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (1:100) (red).)
DLL3, Polyclonal Antibody (Cat# AAA26850)
Full Name
DLL3, CT (DLL3, Delta-like protein 3, Drosophila Delta homolog 3) (APC)
Gene Names
DLL3; SCDO1
Reactivity
Human
Applications
IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(PLD6 Antibody (Center) (Cat# AAA28684) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the PLD6 antibody detected the PLD6 protein (arrow).)
PLD6, Polyclonal Antibody (Cat# AAA28684)
Full Name
PLD6 Antibody (Center)
Gene Names
PLD6; ZUC
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IP (Immunoprecipitation)
(Immunoprecipitating GFP in 293F whole cell lysate transfected with GFPLane 1: Mouse control IgG2b instead of AAA28064 in 293F whole cell lysate transfected with GFPLane 2: AAA28064 (4ug) + 293F whole cell lysate transfected with GFP (500ug)Lane 3: 293F whole cell lysate transfected with GFP (5ug)For western blotting, the blot was detected with AAA28064 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000)
GFP, Monoclonal Antibody (Cat# AAA28064)
Full Name
GFP Monoclonal Antibody
Reactivity
All
Applications
EIA, WB, IF, FC/FACS, IP
Purity
>95%,Protein G purified
Pricing
FCM (Flow Cytometry)
(SOCS1 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SOCS1, Polyclonal Antibody (Cat# AAA28752)
Full Name
SOCS1 Antibody (N-term)
Gene Names
SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
Reactivity
Mouse, rat
Applications
WB, EIA, IHC, IF, FC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(Validation of PKR in Rat Lung Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-PKR antibody (AAA10917) at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
PKR, Polyclonal Antibody (Cat# AAA10917)
Full Name
PKR Antibody
Gene Names
EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF
Purity
PKR Antibody is affinity chromatography purified via peptide column.
Pricing
IP (Immunoprecipitation)
(Figure 13 Immunoprecipitation Validation in HEK293 cells (Kawai et al., 2004)HEK293 cells were transiently transfected with DYKDDDDK-IRF7. Ccell lysates were immunoprecipitated with control rabbit anti-mouse immunoglobulin serum (IgG) or anti-MyD88 (Ab1 and Ab2), followed by immunoblotting with anti-DYKDDDDK.)
MYD88, Polyclonal Antibody (Cat# AAA10930)
Full Name
MYD88 Antibody
Gene Names
MYD88; MYD88D
Reactivity
Human, Mouse
Applications
EIA, WB, IHC, IF
Purity
MYD88 Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(Figure 10 Immunofluorescence images of PUMA in the P14 rat retina (Wakabayashi et al., 2012)PUMA expression in the rat retina detected by anti-PUMA antibodies (3043). The specimens were counterstained with Hoechst 33258 to visualize nuclei (+DNA). GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer; P, postnatal day.)
PUMA, Polyclonal Antibody (Cat# AAA10934)
Full Name
PUMA Antibody
Gene Names
BBC3; JFY1; PUMA; JFY-1
Reactivity
Human
Applications
EIA, WB, IHC, IF
Purity
PUMA Antibody is affinity chromatography purified via peptide column.
Pricing
WB (Western Blot)
(Figure 9 KD Validation in HeLa Cells (Horinaka et al., 2005)HeLa cells were transfected with DR4siRNA or LacZ control siRNA. At 24 h after transfection, the cells were treated with or without 20 μM luteolin for 24 h. Western blot analysis was carried out with anti-DR4 antibodies. DR4 expression was markedly reduced after DR4 knockdown.)
DR4, Polyclonal Antibody (Cat# AAA10950)
Full Name
DR4 Antibody
Gene Names
TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
Reactivity
Human
Applications
EIA, WB
Purity
DR4 Antibody is affinity chromatography purified via peptide column.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human brain section using Pink 1 (AAA28759). AAA28759 was diluted at 1:1000 dilution. A undiluted binotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining)
S100B, Polyclonal Antibody (Cat# AAA28759)
Full Name
S100B Antibody
Gene Names
S100B; NEF; S100; S100-B; S100beta
Reactivity
Human, Mouse, Rat (Predicted: Rabbit)
Applications
WB, IHC-P-Leica, FC/FACS, IHC, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of MCF-7 cell stained with BHLH3 Antibody (N-term). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BHLH3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).BHLH3 immunoreactivity is localized to nucleus significantly.)
BHLH3, Polyclonal Antibody (Cat# AAA28734)
Full Name
BHLH3 Antibody (N-term)
Gene Names
BHLHE41; DEC2; hDEC2; BHLHB3; SHARP1
Reactivity
Human, Mouse
Applications
FC, IF, WB, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
Application Data
(Figure 10 KD Validation in HeLa cells (Horinaka et al., 2005)HeLa cells were transfected with DR4siRNA or LacZ control siRNA. At 24 h after transfection, the cells were treated with or without 20 ?M luteolin for 24 h. Western blot analysis was carried out with anti-DR4 antibodies (1139). DR4 expression was markedly reduced after DR4 knockdown.)
DR4, Polyclonal Antibody (Cat# AAA10952)
Full Name
DR4 Antibody
Gene Names
TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
Reactivity
Human
Applications
EIA, WB, ICC
Purity
DR4 Antibody is Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of GAPDH Antibody (C-term R248) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
GAPDH, Polyclonal Antibody (Cat# AAA28693)
Full Name
GAPDH Antibody (C-term R248)
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
